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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
01/02/2010 |
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Data da última atualização: |
18/03/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARON, L. G.; PIÑEROS, M. A.; GUIMARAES, C. T.; MAGALHAES, J. V. de; PLEIMAN, J. K.; MAO, C.; SHAFF, J.; BELICUAS, S. N. J; KOCHIAN, L. V. |
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Afiliação: |
Lyza G. Maron, Cornell University; Miguel A. Piñeros, Cornell University; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; Jennifer K. Pleiman, Cornell University; Chuanzao Mao, Zheijang University; Jon Shaff, Cornell University; SILVIA NETO JARDIM, CNPMS; Leon V. Kochian, Cornell University. |
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Título: |
Two functionally distinct members of the MATE (multi-drug and toxic compound extrusion) family of transporters potentially underlie two major aluminum tolerance QTLs in maize. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
The Plant Journal, Oxford, v. 61, n. 5, p. 728-740, 2010. |
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DOI: |
10.1111/j.1365-313X.2009.04103.x |
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Idioma: |
Inglês |
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Conteúdo: |
Crop yields are significantly reduced by aluminum (Al) toxicity on acidic soils, which comprise up to 50% of the world?s arable land. Al-activated release of ligands (such as organic acids) from the roots is a major Al tolerance mechanism in plants. In maize, Al-activated root citrate exudation plays an important role in tolerance. However, maize Al tolerance is a complex trait involving multiple genes and physiological mechanisms. Recently, transporters from the MATE family have been shown to mediate Al-activated citrate exudation in a number of plant species. Here we describe the cloning and characterization of two MATE family members in maize, ZmMATE1 and ZmMATE2, which co-localize to major Al tolerance QTL. Both genes encode plasma membrane proteins that mediate significant anion efflux when expressed in Xenopus oocytes. ZmMATE1 expression is mostly concentrated in root tissues, is up-regulated by Al and is significantly higher in Al-tolerant maize genotypes. In contrast, ZmMATE2 expression is not specifically localized to any particular tissue and does not respond to Al. [14C]-citrate efflux experiments in oocytes demonstrate that ZmMATE1 is a citrate transporter. In addition, ZmMATE1 expression confers a significant increase in Al tolerance in transgenic Arabidopsis. Our data suggests that ZmMATE1 is a functional homolog of the Al tolerance genes recently characterized in sorghum, barley and Arabidopsis, and is likely to underlie the largest maize Al tolerance QTL found on chromosome 6. However, ZmMATE2 most likely does not encode a citrate transporter, and could be involved in a novel Al tolerance mechanism. MenosCrop yields are significantly reduced by aluminum (Al) toxicity on acidic soils, which comprise up to 50% of the world?s arable land. Al-activated release of ligands (such as organic acids) from the roots is a major Al tolerance mechanism in plants. In maize, Al-activated root citrate exudation plays an important role in tolerance. However, maize Al tolerance is a complex trait involving multiple genes and physiological mechanisms. Recently, transporters from the MATE family have been shown to mediate Al-activated citrate exudation in a number of plant species. Here we describe the cloning and characterization of two MATE family members in maize, ZmMATE1 and ZmMATE2, which co-localize to major Al tolerance QTL. Both genes encode plasma membrane proteins that mediate significant anion efflux when expressed in Xenopus oocytes. ZmMATE1 expression is mostly concentrated in root tissues, is up-regulated by Al and is significantly higher in Al-tolerant maize genotypes. In contrast, ZmMATE2 expression is not specifically localized to any particular tissue and does not respond to Al. [14C]-citrate efflux experiments in oocytes demonstrate that ZmMATE1 is a citrate transporter. In addition, ZmMATE1 expression confers a significant increase in Al tolerance in transgenic Arabidopsis. Our data suggests that ZmMATE1 is a functional homolog of the Al tolerance genes recently characterized in sorghum, barley and Arabidopsis, and is likely to underlie the largest maize Al tolerance QTL foun... Mostrar Tudo |
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Palavras-Chave: |
Tolerância. |
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Thesagro: |
Alumínio; Milho; Zea mays. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02522naa a2200277 a 4500
001 1631894
005 2019-03-18
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1365-313X.2009.04103.x$2DOI
100 1 $aMARON, L. G.
245 $aTwo functionally distinct members of the MATE (multi-drug and toxic compound extrusion) family of transporters potentially underlie two major aluminum tolerance QTLs in maize.$h[electronic resource]
260 $c2010
520 $aCrop yields are significantly reduced by aluminum (Al) toxicity on acidic soils, which comprise up to 50% of the world?s arable land. Al-activated release of ligands (such as organic acids) from the roots is a major Al tolerance mechanism in plants. In maize, Al-activated root citrate exudation plays an important role in tolerance. However, maize Al tolerance is a complex trait involving multiple genes and physiological mechanisms. Recently, transporters from the MATE family have been shown to mediate Al-activated citrate exudation in a number of plant species. Here we describe the cloning and characterization of two MATE family members in maize, ZmMATE1 and ZmMATE2, which co-localize to major Al tolerance QTL. Both genes encode plasma membrane proteins that mediate significant anion efflux when expressed in Xenopus oocytes. ZmMATE1 expression is mostly concentrated in root tissues, is up-regulated by Al and is significantly higher in Al-tolerant maize genotypes. In contrast, ZmMATE2 expression is not specifically localized to any particular tissue and does not respond to Al. [14C]-citrate efflux experiments in oocytes demonstrate that ZmMATE1 is a citrate transporter. In addition, ZmMATE1 expression confers a significant increase in Al tolerance in transgenic Arabidopsis. Our data suggests that ZmMATE1 is a functional homolog of the Al tolerance genes recently characterized in sorghum, barley and Arabidopsis, and is likely to underlie the largest maize Al tolerance QTL found on chromosome 6. However, ZmMATE2 most likely does not encode a citrate transporter, and could be involved in a novel Al tolerance mechanism.
650 $aAlumínio
650 $aMilho
650 $aZea mays
653 $aTolerância
700 1 $aPIÑEROS, M. A.
700 1 $aGUIMARAES, C. T.
700 1 $aMAGALHAES, J. V. de
700 1 $aPLEIMAN, J. K.
700 1 $aMAO, C.
700 1 $aSHAFF, J.
700 1 $aBELICUAS, S. N. J
700 1 $aKOCHIAN, L. V.
773 $tThe Plant Journal, Oxford$gv. 61, n. 5, p. 728-740, 2010.
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Embrapa Milho e Sorgo (CNPMS) |
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| 12. |  | MEDEIROS, S. R.; OLIVEIRA, D. E.; AROEIRA, L. J. M.; MCGUIRE, M.; LANNA, D. P. D. Supplementation of lactating cows grazing stargrass (Cynodon nlenfuensis var. nlenfuensis) with conjugated linoleic acid: effects on persistency and estimated net energy balance. In: INTERNATIONAL GRASSLAND CONGRESS, 19., 2001, São Pedro, SP. Grassland ecosystems: an outlook into the 21st century: proceedings. Piracicaba: Fealq: SBZ, 2001.| Tipo: Artigo em Anais de Congresso |
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| 13. | | LEME, P. R.; LANNA, D. P. D.; HENRIQUE, W.; ALLEONI, G. F.; BOIN, C. Substituição do milho pela polpa de citros em dietas de bovinos confinados. II. Composição química corporal e taxas de deposição. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora, MG. Anais... Juiz de Fora: SBZ, 1997. p. 358-360. Titulo em inglês: Effect of substituing corn grain for pulp citrus on feeflot steers. II. Body chemical composition and accreation rates of body chemical components.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 15. | | OLIVEIRA, D. E.; MEDEIROS, S. R. de; LANNA, D. P. D. Nitrogen balance and excretion from grazing lactating cows supplemented with conjugated linoleic acid (CLA). In: ADSA-PSA-AMPA-ASAS JOINT ANNUAL MEETING, 2008, Indianapolis. Abstracts. Indianapolis: ADSA: ASAS, 2008. Disponível em:< http://adsa.asas.org/meetings/2008/abstracts/TOC.htm > Acesso em: 26 mar. 2009. Publicado em: Journal of Animal Science, Champaign, v. 86, E-Supplement 2; Journal of Dairy Science, Champaign, v. 91, E-Supplement 1. P. 487 1 CD-ROM. Ruminant Nutrition: proteins and amino acids - Dairy. O485. Trabalho TH253.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 18. | | CRUZ, G. M. da; TULLIO, R. R.; ALLEONI, G. F.; BERNDT, A.; ALENCAR, M. M. de; LANNA, D. P. D. Ganho de peso e conversão alimentar de bovino jovem não-cadastrado de quatro grupos genéticos em confinamento em relação ao status nutricional na fase de pastejo. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 40., 2003, Santa Maria, RS. Anais... Santa Maria: SBZ, 2003. 1 CD-ROM. 5f. 5p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 117 | |
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