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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
06/03/2015 |
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Data da última atualização: |
06/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SICHERLE, C. C.; MAIA, M. da S.; BICUDO, S. D.; RODELLO, L.; AZEVEDO, H. C. |
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Afiliação: |
C. C. SICHERLE, São Paulo State University, Faculty of Veterinary Medicine and Animal Science; MARCIANE DA SILVA MAIA, CPATSA; S. D. BICUDO, São Paulo State University, Faculty of Veterinary Medicine and Animal Science; L. RODELLO, São Paulo State University, Faculty of Veterinary Medicine and Animal Science; H. C. AZEVEDO, São Paulo State University, Faculty of Veterinary Medicine and Animal Science. |
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Título: |
Lipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen supplemented with catalase or Trolox. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 95, p. 144-149, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Semen manipulation and cryopreservation-thaw proceduresmayaccelerate the generation of reactive oxygen species (ROS). Sperm exposure to large amounts of ROS has been shown to cause membrane lipid peroxidation and cellular injury to the sperm. The objective of this study was to overcome the ROS production in frozen-thawed ram semen by the addition of the antioxidants catalase or Trolox to semen following thawing. Frozen-thawed ram semen (100×106 sperm/straw) was supplemented with PBS (control group), 100g/ml catalase, or 100MTrolox/108 sperm (catalase and Trolox being dissolved in PBS) and incubated (37 ◦C) for 5 min. Under the experimental conditions used in this study, the catalase and Trolox antioxidants failed to protect the sperm from the spontaneous production of ROS. However, when lipid peroxidation was induced by iron (FeSO4), the addition of Trolox promoted a reduction (P < 0.05) in the formation of TBARS in the semen, compared to the control and catalase semen samples. The generation of TBARS and H2O2 occurred in the extender alone, without the presence of sperm cells. In conclusion, the addition of Trolox to frozen-thawed ram semen could be beneficial as it decreases the production of TBARS when oxidative stress is induced. It is possible that a longer incubation period could lead to different results. The concentration of catalase also needs to be further evaluated. The extender could contribute to the oxidative stress of sperm, as it is a source of ROS during the cryopreservation of semen. MenosSemen manipulation and cryopreservation-thaw proceduresmayaccelerate the generation of reactive oxygen species (ROS). Sperm exposure to large amounts of ROS has been shown to cause membrane lipid peroxidation and cellular injury to the sperm. The objective of this study was to overcome the ROS production in frozen-thawed ram semen by the addition of the antioxidants catalase or Trolox to semen following thawing. Frozen-thawed ram semen (100×106 sperm/straw) was supplemented with PBS (control group), 100g/ml catalase, or 100MTrolox/108 sperm (catalase and Trolox being dissolved in PBS) and incubated (37 ◦C) for 5 min. Under the experimental conditions used in this study, the catalase and Trolox antioxidants failed to protect the sperm from the spontaneous production of ROS. However, when lipid peroxidation was induced by iron (FeSO4), the addition of Trolox promoted a reduction (P < 0.05) in the formation of TBARS in the semen, compared to the control and catalase semen samples. The generation of TBARS and H2O2 occurred in the extender alone, without the presence of sperm cells. In conclusion, the addition of Trolox to frozen-thawed ram semen could be beneficial as it decreases the production of TBARS when oxidative stress is induced. It is possible that a longer incubation period could lead to different results. The concentration of catalase also needs to be further evaluated. The extender could contribute to the oxidative stress of sperm, as it is a source of ROS duri... Mostrar Tudo |
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Palavras-Chave: |
Semen cryopreservation; Sêmen ovino congelado-descongelado. |
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Thesagro: |
Catalase; Ovino; Produção animal. |
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Thesaurus Nal: |
ANimal production; Lipid peroxidation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02313naa a2200253 a 4500
001 2010784
005 2015-03-06
008 2011 bl uuuu u00u1 u #d
100 1 $aSICHERLE, C. C.
245 $aLipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen supplemented with catalase or Trolox.$h[electronic resource]
260 $c2011
520 $aSemen manipulation and cryopreservation-thaw proceduresmayaccelerate the generation of reactive oxygen species (ROS). Sperm exposure to large amounts of ROS has been shown to cause membrane lipid peroxidation and cellular injury to the sperm. The objective of this study was to overcome the ROS production in frozen-thawed ram semen by the addition of the antioxidants catalase or Trolox to semen following thawing. Frozen-thawed ram semen (100×106 sperm/straw) was supplemented with PBS (control group), 100g/ml catalase, or 100MTrolox/108 sperm (catalase and Trolox being dissolved in PBS) and incubated (37 ◦C) for 5 min. Under the experimental conditions used in this study, the catalase and Trolox antioxidants failed to protect the sperm from the spontaneous production of ROS. However, when lipid peroxidation was induced by iron (FeSO4), the addition of Trolox promoted a reduction (P < 0.05) in the formation of TBARS in the semen, compared to the control and catalase semen samples. The generation of TBARS and H2O2 occurred in the extender alone, without the presence of sperm cells. In conclusion, the addition of Trolox to frozen-thawed ram semen could be beneficial as it decreases the production of TBARS when oxidative stress is induced. It is possible that a longer incubation period could lead to different results. The concentration of catalase also needs to be further evaluated. The extender could contribute to the oxidative stress of sperm, as it is a source of ROS during the cryopreservation of semen.
650 $aANimal production
650 $aLipid peroxidation
650 $aCatalase
650 $aOvino
650 $aProdução animal
653 $aSemen cryopreservation
653 $aSêmen ovino congelado-descongelado
700 1 $aMAIA, M. da S.
700 1 $aBICUDO, S. D.
700 1 $aRODELLO, L.
700 1 $aAZEVEDO, H. C.
773 $tSmall Ruminant Research$gv. 95, p. 144-149, 2011.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 26 | |
| 7. |  | KAWAS, J. R.; GUIMARAES, W.; BARROS, N. N.; SANCHEZ, O.; SHELTON, J. M.; JOHNSON, W. L. Interrelação energia-proteína no crescimento de ovinos deslanados. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 23., 1986, Campo Grande. Anais... Campo Grande: Sociedade Brasileira de Zootecnia, 1986. p. 95.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | BARROS, N. N.; KAWAS, J. R.; JOHNSON, W. L.; SHELTON, J. M.; SOUZA NETO, J. de. Influência da energia do desempenho de cordeiros Somalis alimentados com capim elefante (Pennisetum purpureum). In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 25., 1988, Viçosa, MG. Anais... Viçosa, MG: Sociedade Brasileira de Zootecnia, 1988. p. 114.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | KAWAS, J. R.; LOPES, J.; DANELON, D. L.; LU, C. D. Influence of forage-to-concentrate ratios on intake, digestibility, chewing and milk production of dairy goats. Small Ruminant Research, v. 4, n. 1, p. 11-18, 1991.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | SCHACHT, W. H.; KAWAS, J. R.; MALECHEK, J. C.; FREIRE, L. C. L. Efeito da suplementação de energia e nitrogênio no ganho de peso de caprinos em pastagem nativa (caatinga) na época seca, no nordeste do Brasil. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 22., 1985, Balneário Camboriu, SC. Anais... Balneário Camboriú: Sociedade Brasileira de Zootecnia, 1985. p. 62.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 12. | | SILVA, F. L. R. da; ARRUDA, F. de A. V.; KAWAS, J. R. Efeito do sombreamento sobre o consumo de alimento e água, em ovinos da raça Santa Inês Vermelha do Nordeste. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 25., 1988, Viçosa, MG. Anais... Viçosa, MG: Sociedade Brasileira de Zootecnia, 1988. p. 302.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | LU, C. D.; OSBORN, R. G.; KAWAS, J. R.; ARRUDA, F. Prediction of dry matter intake in lactating and growing dairy goats. In: INTERNATIONAL CONFERENCE ON GOATS, 4., 1987, Brasília, DF. Proceedings... Brasília, DF: EMBRAPA-DDT, 1987. p. 1386. Resumo 121.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | KAWAS, J. R.; GUIMARAES, W.; BARROS, N. N.; FREIRE, L. C. L.; FOOTE, W. C. Survival and growth of goat kids grazing native range from dams supplemented with incremental energy levels. In: INTERNATIONAL CONFERENCE ON GOATS, 4., 1987, Brasília, DF. Proceedings... Brasília, DF: EMBRAPA-DDT, 1987. p. 1399. Abstract 139.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | BARROS, N. N.; KAWAS, J. R.; LOPES, E. A.; JOHNSON, W. L. Estudo comparativo da digestibilidade de leguminosa nativa com caprinos e ovinos, no semi-árido do Estado do Ceará. II. Digestibilidade in vivo da silagem de mata-pasto (Cassia ssp). Pesquisa Agropecuária Brasileira, Brasília, DF, v. 27, n. 11, p. 1551-1555, nov., 1992.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | BARROS, N. N.; FREIRE, L. C. L.; LOPES, E. A.; KAWAS, J.; JOHNSON, W. L. Estudo comparativo da digestibilidade de leguminosa nativa com caprinos e ovinos do sertão cearense. II. Digestibilidade in vivo do feno de mata-pasto. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 26, n. 8, p. 1215-1218, 1991.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | BARROS, N. N.; KAWAS, J.; JOHNSON, W. L.; SHELTON, J. M. Energy utilization by Somalis lambs fed napiergrass "ad libitum" and an energy supplement at incremental levels. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 25, n. 9, p. 1283-1291, set., 1990.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | BARROS, N. N.; OLIVEIRA, E. R.; ROBB, T. W.; ARRUDA, F. A. V.; KAWAS, J. R.; JOHNSON, W. L. Acabamento de caprinos e ovinos durante a época seca, no Nordeste. In: REUNIÃO TÉCNICO-CIENTÍFICA DO PROGRAMA DE APOIO A PESQUISA COLABORATIVA DE PEQUENOS RUMINANTES, 1., 1986. Sobral. Anais... Sobral: EMBRAPA-CNPC; SR-CRSP, 1986. p. 285-295. (EMBRAPA-CNPC. Documentos, 6).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | BARROS, N. N.; KAWAS, J. R.; FREIRE, L. C. L.; JOHNSON, W. L.; SANTOS, J. W. dos. Nutritive value of total mixed rations with various energy sources fed to lambs, in Northeast Brazil. Journal of Animal Science, v. 61, suppl.1, p. 452, 1985. Abstract 592. Abstracts of the 77 ASAS Annual Meeting, Biloxi, Mississipi, 1985.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 26 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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