| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
29/08/2002 |
|
Data da última atualização: |
07/06/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
LOGUERCIO, L. L.; BARRETO, M. L.; ROCHA, T. L.; SANTOS, C. G.; TEIXEIRA, F. F.; PAIVA, E. |
|
Afiliação: |
FLAVIA FRANCA TEIXEIRA, CNPMS. |
|
Título: |
Combined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm. |
|
Ano de publicação: |
2002 |
|
Fonte/Imprenta: |
Journal of Applied Microbiology, Danvers, v. 93, n. 2, p. 269-277, 2002. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Aims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolated. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and impact of the study: a simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool forr strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm. |
|
Thesagro: |
Genética. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02123naa a2200193 a 4500
001 1485545
005 2018-06-07
008 2002 bl uuuu u00u1 u #d
100 1 $aLOGUERCIO, L. L.
245 $aCombined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm.$h[electronic resource]
260 $c2002
520 $aAims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolated. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and impact of the study: a simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool forr strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm.
650 $aGenética
700 1 $aBARRETO, M. L.
700 1 $aROCHA, T. L.
700 1 $aSANTOS, C. G.
700 1 $aTEIXEIRA, F. F.
700 1 $aPAIVA, E.
773 $tJournal of Applied Microbiology, Danvers$gv. 93, n. 2, p. 269-277, 2002.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 6 | |
| 2. |  | HARTEMINK, A. E.; McBRATNEY, A. B.; HEMPEL, J.; MENDONÇA-SANTOS, M. de L.; McKENZIE, N.; SANCHEZ, P.; GAN-LIN, Z.; MONTANARELLA, L. GlobalSoilMap.net: a new digital soil map of the world. MEA Bulletin, n. 71, jun. 2009. 2 p.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Solos. |
|    |
| 3. | | HARTEMINK, A. E.; HEMPEL, J.; LAGACHERIE, P.; MCBRATNEY, A.; MCKENZIE, N.; MACMILLAN, R. A.; MINASNY, B.; MONTANARELLA, L.; MENDONÇA-SANTOS, M. de L.; SANCHEZ, P.; WALSH, M.; ZHANG, G. GlobalSoilMap.net: a new digital soil map of the world. In: BOETTINGER, J. L.; HOWELL, D. W.; MOORE, A. C.; HARTEMINK, A. E.; KIENAST-BROWN, S. (Ed.). Digital soil mapping: bridging research, environmental application, and operation. New York: Springer, 2010. p. 423-427.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Solos. |
|  |
| 4. | | SANCHEZ, P. A.; AHAMED, S.; CARRÉ, F.; HARTEMINK, A. E.; HEMPEL, J.; HUISING, J.; LAGACHERIE, P.; MACBRATNEY, A. B.; MCKENZIE, N. J.; MENDONÇA-SANTOS, M. de L.; MINASNY, B.; MONTANARELLA, L.; OKOTH, P.; PALM, C. A.; SACHS, J. D.; SHEPHERD, K. D.; VAGEN, T. G.; VANLAUWE, B.; WALSH, M. G.; WINOWIECKI, L. A.; ZHANG, G. L. Digital soil map of the world. Science, v. 325, n. 5941, p. 680-681, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
| |
| 5. | | ARROUAYS, D.; GRUNDY, M. G.; HARTEMINK, A. E.; HEMPEL, J. W.; HEUVELINK, G. B. M.; HONG, S. Y.; LAGACHERIE, P.; LELYK, G.; MCBRATNEY, A. B.; MCKENZIE, N. J.; MENDONCA-SANTOS, M. D. L.; MINASNY, B.; MONTANARELLA, L.; ODEH, I. O. A.; SANCHEZ, P. A.; THOMPSON, J. A.; ZHANG, G.-L. GlobalSoilMap: toward a fine-resolution global grid of soil properties. Advances in Agronomy, v. 125, p. 93-134, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
| |
| 6. | | ARROUAYS, D.; LEENAARS, J. G. B.; RICHER-DE-FORGES, A. C.; ADHIKARI, K.; BALLABIO, C.; GREVE, M.; GRUNDY, M.; GUERRERO, E.; HEMPEL, J.; HENGL, T.; HEUVELINK, G.; BATJES, N.; CARVALHO, E.; HARTEMINK, A.; HEWITT, A.; HONG, S.-Y.; KRASILNIKOV, P.; LAGACHERIE, P.; LELYK, G.; LIBOHOVA, Z.; LILLY, A.; MCBRATNEY, A.; MCKENZIE, N.; VASQUES, G. de M. Soil legacy data rescue via GlobalSoilMap and other international and national initiatives. GeoResJ, v. 14, p. 1-19, Dec. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Solos. |
| |
| Registros recuperados : 6 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
