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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/12/2017 |
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Data da última atualização: |
04/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ERGUN; SOUZA, F. V. D. |
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Afiliação: |
ERGUN KAYA, Mugla Sitki Kocman University; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Comparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, v.53, Issue 4,p 410?417,| August 2017. |
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ISSN: |
1475-2689 |
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DOI: |
10.1007/s11627-017-9837-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Conservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stability for all cryopreserved varieties. MenosConservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stab... Mostrar Tudo |
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Thesagro: |
Criopreservação. |
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Categoria do assunto: |
-- |
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Marc: |
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022 $a1475-2689
024 7 $a10.1007/s11627-017-9837-2$2DOI
100 1 $aERGUN
245 $aComparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers.$h[electronic resource]
260 $c2017
520 $aConservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stability for all cryopreserved varieties.
650 $aCriopreservação
700 1 $aSOUZA, F. V. D.
773 $tIn Vitro Cellular & Developmental Biology - Plant$gv.53, Issue 4,p 410?417,| August 2017.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 32 | |
| 1. |  | MANFROI, E.; ROESLER, E.; YAMAZAKI-LAU, E.; GRANDO, M. F. Temperatura de cocultivo na transformação transiente de trigo mediada por Agrobacterium tumefaciens. In: MOSTRA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA TRIGO, 8.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 5., 2013, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2013. p. 57. Editores técnicos: Joseani Mesquita Antunes, Ana Lídia Variani Bonato, Márcia Barrocas Moreira Pimentel.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 5. | | MANFROI, E.; YAMAZAKI-LAU, E.; GRANDO, M. F.; ROESLER, E. A. Acetosyringone, pH and temperature effects on transient genetic transformation of immature embryos of Brazilian wheat genotypes by Agrobacterium tumefaciens. Genetics and Molecular Biology, Ribeirão Preto, v. 38, n. 4, p. 470-476, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Trigo. |
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| 6. | | ROESLER, E. A.; MANFROI, E.; MORAS, A.; DIDONÉ, D. A.; GRANDO, M. F.; YAMAZAKI-LAU, E. Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes. Ciência Rural, v. 48, n. 11, p. 1-8, e20170806, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Trigo. |
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| 8. | | VANCINI, C.; TORRES, G. A. M.; CONSOLI, L.; GRANDO, M. F.; DE BONA, F. D. Análise de gluteninas de alto peso molecular como método complementar para determinação da homogeneidade e detecção de misturas em amostras de genótipos de trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 12.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 9., 2017, Passo Fundo. Resumos... Passo Fundo: Embrapa Trigo, 2017. p. 46.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 9. | | TONIAZZO, C.; BRAMMER, S. P.; MINELLA, E.; CAVERZAN, A.; WIETHÖLTER, P.; CASASSOLA, A.; GRANDO, M. F. Malting quality and genetic diversity in brazilian elite barley germplasm. International Journal of Current Research, v. 9, n. 6, p. 52352-52357, June 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Trigo. |
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| 10. | | VANCINI, C.; GRANDO, M. F.; TORRES, G. A. M.; MIRANDA, M. Z. de; CONSOLI, L. Associação de gluteninas de alto peso molecular com a qualidade tecnológica de trigo no Brasil. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 11.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 8., 2016, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2016. p. 49.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 12. | | VANCINI, C.; TORRES, G. A. M.; CONSOLI, L.; GRANDO, M. F. Caracterização de alelos de gluteninas de alto peso molecular em trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 13.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 10., 2018, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2018. p. 48. Pós-graduação.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 15. | | VANCINI, C.; TORRES, G. A. M.; CONSOLI, L.; CRESPI, T. B.; GRANDO, M. F. Eficiência de marcadores moleculares de DNA pelo método Kasp na identificação de alelos de HMW-GS. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 12., 2018, Passo Fundo. Ata e Resumos... Passo Fundo: Projeto Passo Fundo, 2019. Melhoramento, Aptidão Industrial e Sementes, p. 521-525.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 16. | | COELHO, M. B.; SCAGLIUSI, S. M. M.; LIMA, M. I. P. M.; GRANDO, M. F. Efeito do pré-tratamento de espigas na indução da androgênese em trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 11.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 8., 2016, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2016. p. 48.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 18. | | COELHO, M. B.; SCAGLIUSI, S. M. M.; LIMA, M. I. P. M.; CONSOLI, L.; GRANDO, M. F. Androgenic response of wheat genotypes resistant to fusariosis. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 5, p. 575-582, maio, 2018. Título em português: Resposta androgênica de genótipos de trigo resistentes à fusariose.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Trigo; Embrapa Unidades Centrais. |
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| 19. | | GRANDO, M. F.; CASTRO, B. L.; SILVA, M. R.; YAMAZAKI-LAU, E.; KAZMIRSKI, T.; SUZIN, M.; ALVES, F. L. Curva de morte dos calos embriogênicos de uma linhagem brasileira de milho utilizando diferentes concentrações do agente seletivo Bialaphos. In: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2010. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 20. | | COSTA, L. O.; CECHIN, J.; RIZZARDI, M. A.; MARTIN, S. L.; SAUDER, C. A.; ADEGAS, F. S.; GRANDO, M. F. Target-site resistance and cross-resistance to ALS-inhibiting herbicides in radish and wild radish biotypes from Brazil. Agronomy Journal, v. 113, p. 236-249, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 32 | |
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