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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
16/12/2013 |
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Data da última atualização: |
10/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, G. S.; NUNES, E. S.; LAPERUTA, L. D. C.; BRAGA, M. F.; PENHA, H. A.; DINIZ, A. L.; MUNHOZ, C. F.; GAZAFFI, R.; GARCIA, A. A. F.; VIEIRA, M. L. C. |
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Afiliação: |
ESALQ/USP; ESALQ/USP; ESALQ/USP; MARCELO FIDELES BRAGA, CPAC; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP. |
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Título: |
Molecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Annals of Applied Biology, v. 162, n. 3, p. 347-361, 2013. |
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ISSN: |
0003-4746 |
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DOI: |
10.1111/aab.12028 |
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Idioma: |
Inglês |
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Conteúdo: |
One of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0 cM, with an average distance between adjacent markers of 3.8 cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping. MenosOne of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of w... Mostrar Tudo |
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Thesagro: |
Maracujá; Marcador genético; Marcador molecular; Melhoramento genético vegetal; Polimorfismo Genético. |
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Thesaurus Nal: |
Genetic improvement; Genetic markers; Genetic polymorphism; Passiflora alata; Passion fruits. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02903naa a2200373 a 4500
001 1973873
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008 2013 bl uuuu u00u1 u #d
022 $a0003-4746
024 7 $a10.1111/aab.12028$2DOI
100 1 $aPEREIRA, G. S.
245 $aMolecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species.
260 $c2013
520 $aOne of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0 cM, with an average distance between adjacent markers of 3.8 cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping.
650 $aGenetic improvement
650 $aGenetic markers
650 $aGenetic polymorphism
650 $aPassiflora alata
650 $aPassion fruits
650 $aMaracujá
650 $aMarcador genético
650 $aMarcador molecular
650 $aMelhoramento genético vegetal
650 $aPolimorfismo Genético
700 1 $aNUNES, E. S.
700 1 $aLAPERUTA, L. D. C.
700 1 $aBRAGA, M. F.
700 1 $aPENHA, H. A.
700 1 $aDINIZ, A. L.
700 1 $aMUNHOZ, C. F.
700 1 $aGAZAFFI, R.
700 1 $aGARCIA, A. A. F.
700 1 $aVIEIRA, M. L. C.
773 $tAnnals of Applied Biology$gv. 162, n. 3, p. 347-361, 2013.
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Registro original: |
Embrapa Cerrados (CPAC) |
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| Registros recuperados : 41 | |
| 2. |  | GRAÇA, J. P. da; HOFFMANN-CAMPO, C. B.; SALVADOR, M. C.; MANDARINO, J. M. G. Atividade de lipoxigénases em grãos e aspectos morfológicos das vagens de genótipos de soja (G/yCine max (L.) Merril). In: ENCONTRO BRASILEIRO DE ECOLOGIA QUÍMICA, 5., 2007, Londrina. Resumos... Londrina: Embrapa Soja, 2007. p. 73. Organizado por Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Lenita Jacob Oliveira, Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | GAZOLA, D.; ZUCARELI, C.; RINGENBERG, R.; GRAÇA, J. P. da; HOFFMANN-CAMPO, C. B. Nitrogen fertilization in the contents of secondary compounds in cassava cultivars Adubação nitrogenada nos teores de compostos secundários em cultivares de mandioca industrial. Semina: Ciências Agrárias, Londrina, v. 39, n. 3, p. 1015-1028, maio/jun. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | GAZOLA, D.; ZUCARELI, C.; RINGENBERG, R.; GRAÇA, J. P. da; HOFFMANN-CAMPO, C. B. Nitrogen fertilization in the contents of secondary compounds in cassava cultivars. Semina: Ciências Agrárias, Londrina, v. 39, n. 3, p. 1015-1028, maio/jun. 2018. Título em português: Adubação nitrogenada nos teores de compostos secundários em cultivares de mandioca industrial.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 8. | | GRAÇA, J. P. da; SALVADOR, M. C.; MELLO, D.; MANDARINO, J. M. G.; HOFFMANN-CAMPO, C. B. Enzymatic activity of lipoxygenases, isoflavone contents, and trichomes in soybean genotypes: implication on insect plant resistance. In: ANNIVERSARY ISCE MEETING, 25., 2008, State College. [Abstracts...] State College: International Society of Chemical Ecology, 2008. p. 88.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | GRAÇA, J. P. da; PARPINELLI, R. S.; COTRIM, G.; TOLEDO, V. D.; GAZZONI, D. L.; ZOCOLO, G.; CAMPO, C. B. Floral Anthocyanins in Soybean Cultivars: Detection and Differentiation by Using Uplc-Hrms Strategies. In: SETAC LATIN AMERICA BIENNIAL MEETING, 14., 2021. Latin America, diversity of knowledge for a sustainable future: abstract book. Pensacola: SETAC, 2021. Virtual. p. 8.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Soja. |
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| 12. | | GAZZONI, D. L.; BARATEIRO, J. V.; CAMPO, C. B.; ZOCOLO, G.; GRAÇA, J. P. da; PARPINELLI, R. S. Supplemental Pollination by Apis mellifera Increased Soybean Yield in Brazil. In: SETAC LATIN AMERICA BIENNIAL MEETING, 14., 2021. Latin America, diversity of knowledge for a sustainable future: abstract book. Pensacola: SETAC, 2021. Virtual. p. 7.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 15. | | GAZOLA, D.; ZUCARELI, C.; RINGENBERG, R.; OLIVEIRA, M. C. N. de; GRAÇA, J. P. da; NUNES, E. de O.; HOFFMANN-CAMPO, C. B. Secondary metabolite contents in different parts of cassava plants infested by Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae). Arthropod-Plant Interactions, v. 13, p. 359-366, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 16. | | UEDA, T. E.; GRAÇA, J. P. da; FUJISAO, J. T.; MARTINELLI, S.; CARVALHO, E. A. de; VENTURA, M. U.; HOFFMANN-CAMPO, C. B. Secondary metabolites extracted from Spodoptera frugiperda adult bodies indicators of soybean feeding at larval stage. In: ANNUAL MEETING OF THE INTERNATIONAL SOCIETY OF CHEMICAL ECOLOGY - ISCE, 32.; CONGRESSO OF THE LATIN AMERICAN ASSOCIATION OF CHEMICAL ECOLOGY - ALAEQ, 4.; JOINT MEETING ISCE/ALAEQ, 1., 2016, Iguaçu. Abstracts... Londrina: [s. n.], 2016. p. 147.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 17. | | VIEIRA, S. S.; LOURENÇÃO, A. L.; GRAÇA, J. P. da; JANEGITZ, T.; SALVADOR, M. C.; OLIVEIRA, M. C. N. de; HOFFMANN-CAMPO, C. B. Biological aspects of Bemisia tabaci biotype B and the chemical causes of resistance in soybean genotypes. Arthropod-Plant Interactions, v. 10, n. 6, p. 525-534, Dec. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 18. | | SALVADOR, M. C.; BOIÇA JÚNIOR, A. L.; OLIVEIRA, M. C. N. de; GRAÇA, J. P. da; SILVA, D. M da; HOFFMANN-CAMPO, C. B. Do different casein concentrations increase the adverse effect of rutin on the biology of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae)? Neotropical Entomology, v. 39, n. 5, p. 774-783, Sept./Oct. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 19. | | SCUPINARI, T.; GRAÇA, J. P. da; LOPES, I. de O. N.; DIAS, W. P.; HOFFMANN-CAMPO, C. B.; NUNES, E. de O. Crotalaria spectabilis seeds extract and associated adjuvants: nematicide effect on the Pratylenchus brachyurus juveniles. In: ANNUAL MEETING OF THE INTERNATIONAL SOCIETY OF CHEMICAL ECOLOGY - ISCE, 32.; CONGRESSO OF THE LATIN AMERICAN ASSOCIATION OF CHEMICAL ECOLOGY - ALAEQ, 4.; JOINT MEETING ISCE/ALAEQ, 1., 2016, Iguaçu. Abstracts... Londrina: [s. n.], 2016. p. 121.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 20. | | ABREU, A. de; SALVADOR, M. C.; UEDA, T. E.; GRAÇA, J. P. da; OLIVEIRA, M. C. N. de; HOFFMANN-CAMPO, C. B. Efeito do aumento da temperatura sobre o ciclo biológico e de gerações de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae). In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 8., 2013, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2013. (Embrapa Soja. Documentos, 339). p. 196-203.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 41 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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