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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
17/05/2023 |
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Data da última atualização: |
11/12/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ALVES, T.; GEISSLITZ, S. M.; NASCIMENTO, T. P. DO; CARVALHO, C. W. P. de; SCHEERF, K. A.; TAKEITI, C. Y.; FERREIRA, M. S. L. |
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Afiliação: |
THAIS ALVES, UNIRIO; SABRINA MARIA GEISSLITZ, Karlshuhe Institute of Technology; TATIANA PIMENTA DO NASCIMENTO, UNIRIO; CARLOS WANDERLEI PILER DE CARVALHO, CTAA; KATHARINA ANNE SCHEERF, Karlsruhe Institute of Technology; CRISTINA YOSHIE TAKEITI, CTAA; MARIANA SIMÕES LARRAZ FERREIRA, UNIRIO. |
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Título: |
A proteomic-based study to assess the effect of extrusion in the allergenic profile of wheat flours of different technological qualities. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: CONFERÊNCIA INTERNACIONAL DE PROTEÍNAS E COLOIDES ALIMENTARES,9., 2023, Rio de Janeiro. Anais... Campinas, Galoá, 2023. |
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Idioma: |
Inglês |
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Notas: |
Poster 157774. CIPCA 2023. Colóides alimentares e saúde (peptídeos bioativos, alergenicidade). |
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Conteúdo: |
Although wheat is widely produced and consumed worldwide, predisposed people can present sensitivities or inflammatory reactions to the wheat proteins. These reactions are caused by the stimulation of the immune system of individuals presenting celiac disease, non-celiac gluten sensitivity, wheat allergy, dermatitis herpetiformis, amongst other disorders. This study aimed to understand how wheat flours of contrasting technological end-use qualities submitted to thermoplastic and cold extrusion behave concerning the expression of allergenic proteins. Wheat flours (Triticum aestivum) were extracted from two wheat genotypes of different end-uses (ORS Agile, classified as bread/improver wheat; ORS Vintecinco, classified as biscuit wheat). Then, extrudates were produced using single-screw extruder, applying two different screw rotation speeds (150 and 300 rpm). Pasta was also produced by the cold-extrusion process with further cooking. Proteins were sequentially extracted based on the Osborne solubility classification: salt-soluble (AG), ethanol-soluble (Gli), SDS-soluble with reduction (Glu) and then quantified by RP-HPLC. Extracts were reduced (DTT), alkylated (IAM), and digested (thermolysin, 1:20). Peptides were diluted and analyzed by nanoLC-MS/MS in a Q Exactive Plus Orbitrap by using data?dependent acquisition (DDA) in positive ion mode. MS data was processed in MaxQuant using a Triticum database from UniprotKB using the following parameters: maximum of two missed cleavages, methionine oxidation and N-terminal protein acetylation as variable modifications, cysteine carbamidomethylation as fixed modification, maximum of 5 modifications per peptide, minimal length of 7 amino acids and 1% peptide and protein FDR. Filters (at least 2/3 replicates, score ? 1, no reverses, no potential contaminants) were applied to processed data to assign protein identification. Uni, multivariate and hierarchical clustering analysis were performed, and the identified proteins were searched against a compiled of wheat allergenic proteins built from official allergenic databases available online. After processing proteomic data, a total of 339 proteins were evaluated, from which 250 were present in ORS Agile and 162 in ORS Vintecinco. PCA allowed the separation of samples in three distinct clusters (1: flour; 2: 300 rpm extrudates; 3: 150 rpm extrudates, raw and cooked pasta) with the same pattern for both samples. Flours and expanded extrudates (300 rpm) presented a more distinct profile from the samples in third cluster. In the latter cluster, these samples presented a related protein profile among themselves. Dendrograms and its corresponding heatmaps corroborate, showing flours as the most hierarchically distant from products. Preliminary results concerning the expression of allergenic proteins showed that from the set of identified proteins, only 3% were stablished as wheat allergens. Proteins representative of all fractions (AG, Gli, Glu) were present in this list. From those, five were shared by samples from ORS Agile and ORS Vintecinco. ORS Agile flour had the highest expression intensity from the samples, while ORS Vintecinco expanded extrudate has the lowest. Although interesting and pointing to some effect of processing, further studies are necessary to determine the role of technological quality and processing in causing changes to the allergenic potential of wheat products. MenosAlthough wheat is widely produced and consumed worldwide, predisposed people can present sensitivities or inflammatory reactions to the wheat proteins. These reactions are caused by the stimulation of the immune system of individuals presenting celiac disease, non-celiac gluten sensitivity, wheat allergy, dermatitis herpetiformis, amongst other disorders. This study aimed to understand how wheat flours of contrasting technological end-use qualities submitted to thermoplastic and cold extrusion behave concerning the expression of allergenic proteins. Wheat flours (Triticum aestivum) were extracted from two wheat genotypes of different end-uses (ORS Agile, classified as bread/improver wheat; ORS Vintecinco, classified as biscuit wheat). Then, extrudates were produced using single-screw extruder, applying two different screw rotation speeds (150 and 300 rpm). Pasta was also produced by the cold-extrusion process with further cooking. Proteins were sequentially extracted based on the Osborne solubility classification: salt-soluble (AG), ethanol-soluble (Gli), SDS-soluble with reduction (Glu) and then quantified by RP-HPLC. Extracts were reduced (DTT), alkylated (IAM), and digested (thermolysin, 1:20). Peptides were diluted and analyzed by nanoLC-MS/MS in a Q Exactive Plus Orbitrap by using data?dependent acquisition (DDA) in positive ion mode. MS data was processed in MaxQuant using a Triticum database from UniprotKB using the following parameters: maximum of two missed cleavage... Mostrar Tudo |
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Palavras-Chave: |
Allergenic proteins; LC-MS/MS; Wheat allergy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04321nam a2200229 a 4500
001 2153778
005 2023-12-11
008 2023 bl uuuu u00u1 u #d
100 1 $aALVES, T.
245 $aA proteomic-based study to assess the effect of extrusion in the allergenic profile of wheat flours of different technological qualities.$h[electronic resource]
260 $aIn: CONFERÊNCIA INTERNACIONAL DE PROTEÍNAS E COLOIDES ALIMENTARES,9., 2023, Rio de Janeiro. Anais... Campinas, Galoá$c2023
500 $aPoster 157774. CIPCA 2023. Colóides alimentares e saúde (peptídeos bioativos, alergenicidade).
520 $aAlthough wheat is widely produced and consumed worldwide, predisposed people can present sensitivities or inflammatory reactions to the wheat proteins. These reactions are caused by the stimulation of the immune system of individuals presenting celiac disease, non-celiac gluten sensitivity, wheat allergy, dermatitis herpetiformis, amongst other disorders. This study aimed to understand how wheat flours of contrasting technological end-use qualities submitted to thermoplastic and cold extrusion behave concerning the expression of allergenic proteins. Wheat flours (Triticum aestivum) were extracted from two wheat genotypes of different end-uses (ORS Agile, classified as bread/improver wheat; ORS Vintecinco, classified as biscuit wheat). Then, extrudates were produced using single-screw extruder, applying two different screw rotation speeds (150 and 300 rpm). Pasta was also produced by the cold-extrusion process with further cooking. Proteins were sequentially extracted based on the Osborne solubility classification: salt-soluble (AG), ethanol-soluble (Gli), SDS-soluble with reduction (Glu) and then quantified by RP-HPLC. Extracts were reduced (DTT), alkylated (IAM), and digested (thermolysin, 1:20). Peptides were diluted and analyzed by nanoLC-MS/MS in a Q Exactive Plus Orbitrap by using data?dependent acquisition (DDA) in positive ion mode. MS data was processed in MaxQuant using a Triticum database from UniprotKB using the following parameters: maximum of two missed cleavages, methionine oxidation and N-terminal protein acetylation as variable modifications, cysteine carbamidomethylation as fixed modification, maximum of 5 modifications per peptide, minimal length of 7 amino acids and 1% peptide and protein FDR. Filters (at least 2/3 replicates, score ? 1, no reverses, no potential contaminants) were applied to processed data to assign protein identification. Uni, multivariate and hierarchical clustering analysis were performed, and the identified proteins were searched against a compiled of wheat allergenic proteins built from official allergenic databases available online. After processing proteomic data, a total of 339 proteins were evaluated, from which 250 were present in ORS Agile and 162 in ORS Vintecinco. PCA allowed the separation of samples in three distinct clusters (1: flour; 2: 300 rpm extrudates; 3: 150 rpm extrudates, raw and cooked pasta) with the same pattern for both samples. Flours and expanded extrudates (300 rpm) presented a more distinct profile from the samples in third cluster. In the latter cluster, these samples presented a related protein profile among themselves. Dendrograms and its corresponding heatmaps corroborate, showing flours as the most hierarchically distant from products. Preliminary results concerning the expression of allergenic proteins showed that from the set of identified proteins, only 3% were stablished as wheat allergens. Proteins representative of all fractions (AG, Gli, Glu) were present in this list. From those, five were shared by samples from ORS Agile and ORS Vintecinco. ORS Agile flour had the highest expression intensity from the samples, while ORS Vintecinco expanded extrudate has the lowest. Although interesting and pointing to some effect of processing, further studies are necessary to determine the role of technological quality and processing in causing changes to the allergenic potential of wheat products.
653 $aAllergenic proteins
653 $aLC-MS/MS
653 $aWheat allergy
700 1 $aGEISSLITZ, S. M.
700 1 $aNASCIMENTO, T. P. DO
700 1 $aCARVALHO, C. W. P. de
700 1 $aSCHEERF, K. A.
700 1 $aTAKEITI, C. Y.
700 1 $aFERREIRA, M. S. L.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Biblioteca |
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Registro |
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| Registros recuperados : 24 | |
| 3. |  | BRAGA, M. F.; VIEIRA, M. L. C.; GAZAFFI, R.; GARCIA, A. A. F.; GIRALDI, I. O.; JUNQUEIRA, N. T. V. Mapeamento de QTL (quantitative trait loci) associados à resistência do maracujá-doce à bacteriose. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
|    |
| 4. | | ROSÁRIO, M. F. do; GAZAFFI, R.; MOURA, A. S. A. M. T.; LEDUR, M. C.; GARCIA, A. A. F.; COUTINHO, L. L. Base genética da correlação entre características de desempenho e de rendimento de carcaça no cromossomo 1 da galinha. In: CONFERÊNCIA FACTA DE CIÊNCIA E TECNOLOGIA AVÍCOLAS, 2011, Santos, SP. Anais... Santos: FACTA, 2011. Trabalhos de Pesquisa José Maria Lamas da Silva. 1 CD-ROM. Projeto: 02.09.07.006.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 5. | | ANONI, C. A.; MANCINI, M. C.; COSTA, E. A.; GAZAFFI, R.; PASTINA, M. M.; PINTO, L. R.; SOUZA, A. P.; GARCIA, A. A. F. QTL mapping for yield related trait in a biparental cross of sugarcane. In: CONGRESSO BRASILEIRO DE GENÉTICA, 60., 2014, Guarujá. Resumos. Ribeirão Preto: Sociedade Brasileira de Genética, 2014. p. 126.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 6. | | ROSÁRIO, M. F.; GAZAFFI, R.; MOURA, A. S. A. M. T.; LEDUR, M. C.; COUTINHO, L. L.; GARCIA, A. A. F. QTLs associados a características de carcaça na galinha: mapeamento por intervalo vs mapeamento por intervalo composto. In: CONFERÊNCIA FACTA DE CIÊNCIA E TECNOLOGIA AVÍCOLAS, 27., 2009, Porto Alegre. Anais [dos] trabalhos de pesquisa José Maria Lamas de Silva. [Campinas]: FACTA, 2009. Projeto/Plano de Ação: 01.02.10.210-10. Publicado em CD-ROM (CD00156).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | ROSÁRIO, M. F. do; GAZAFFI, R.; LEDUR, M. C.; MOURA, A. S. A. M. T.; BOSCHIERO, C.; COUTINHO, L. L.; GARCIA, A. A. F. G. QTL's mapeados para características de desempenho na galinha através do mapeamento por intervalo composto com modelo misto. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 7., 2008, São Carlos, SP. Anais. São Carlos, SP: SBMA, 2008. 4 p. Projeto/Plano de Ação: 01.02.102.10-10.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | VIGNA, B. B. Z.; SANTOS, J. C.; PASTINA, M. M.; GAZAFFI, R.; MOLLINARI, M.; CANCADO, L. J.; VALLE, C. B. do; ANTONIO AUGUSTO FRANCO GARCIA; SOUZA, A. P. The Preliminary Genetic Linkage Map of hexaploid Brachiaria humidicola Based on Microsatellite Markers. In: INTERNATIONAL SYMPOSIUM ON FORAGE BREEDING, 3., 2011, Bonito, MS. Breeding forage for climate change adaptation and mitigation - eco-efficient animal produtction: proceedings. [Campo Grande, MS: Embrapa Gado de Corte], 2011. 42-45 1 CD-ROM| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 9. | | ROSÁRIO, M. F. do; GAZAFFI, R.; MOURA, A. S. A. M. T.; LEDUR, M. C.; COUTINHO, L. L.; GARCIA, A. A. S. Composite interval mapping and mixed models reveal QTL associated with performance and carcass traits on chicken chromosomes 1, 3, and 4. Journal of Applied Genetics, Pozna, on line 28 nov. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
|  |
| 11. | | FREITAS, E. G. de; PASTINA, M. M.; GAZAFFI, R.; PINTO, L. R.; XAVIER, M. A.; LANDELL, M. G. de A.; GARCIA, A. A. F. Modelos mistos para seleção de genótipos superiores e de futuros genitores de cana-de-açúcar. In: REUNIÃO ANUAL DA REGIÃO BRASILEIRA DA SOCIEDADE INTERNACIONAL DE BIOMETRIA, 58.; SIMPÓSIO DE ESTATÍSTICA APLICADA À EXPERIMENTAÇÃO AGRONÔMICA, 15., 2013, Campina Grande. Modelagem estatística em áreas multidisciplinares: impactos causados pelas mudanças climáticas na Região Nordeste: anais. Campina Grande: Sociedade Internacional de Biometria, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 12. | | ROSÁRIO, M. F. do; GAZAFFI, R.; MOURA, A. S. A. M. T.; LEDUR, M. C.; NONES, K.; GARCIA, A.; COUTINHO, L. L. Multitrait composite interval mapping reveals pleiotropic QTL on chicken chromosome 1. In: CONFERENCE OF THE INTERNATIONAL SOCIETY FOR ANIMAL GENETICS, 33., 2012, Cairns. Programme and abstract book... Cairns: ISAG, 2012. p.40.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 13. | | LIMA, B. M.; TEIXEIRA, J. E. C.; GAZAFFI, R.; GARCIA, A. A. F.; GRATTAPAGLIA, D.; VALLE, R. K. D.; CAMARGO, L. E. A. Identification of a novel QTL contributing to rust resistance in Eucalyptus. BMC Proceedings 2011, v. 5, Suppl 7, p. P32, 2011. Edição dos resumos do IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration, 2011 Arraial d?Ajuda, Bahia.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | SILVA, F. E. de J.; GUIDO, L. N.; GAZAFFI, R.; GARCIA, A. A. F.; LEDUR, M. C.; COUTINHO, L. L.; ROSÁRIO, M. F. do. Regiões genômicas associadas a características de desempenho e carcaça no cromossomo 5 de linhagens brasileiras de galinha. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 3, p. 229-238, 2011. Projeto: 01.06.01.006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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| 15. | | COSTA, E. A.; ANONI, C. O.; MANCINI, M. C.; SANTOS, F. R. C.; MARCONI, T. G.; GAZAFFI, R.; PASTINA, M. M.; PERECIN, D.; MOLINARI, M.; XAVIER, M. A.; PINTO, L. R.; SOUZA, A. P.; GARCIA, A. A. F. QTL mapping including codominant SNP markers with ploidy level information in a sugarcane progeny. Euphytica, Dordrecht, v. 211, n. 1, p. 1-16, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 16. | | ANONI, C. O.; GAZAFFI, R.; PASTINA, M. M.; MANCINI, M. C.; COSTA, E. A.; MOLLINARI, M.; MARCONI, T. G.; PINTO, L. R.; SOUZA, A. P.; GARCIA, A. A. F. QTL mapping for yield components in a sugarcane commercial cross. In: CONGRESSO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Ribeirão Preto: SBG, 2013. p. 55.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 17. | | MORAIS, L. K. de; CURSI, D. E.; SANTOS, J. M. dos; SAMPAIO, M.; CAMARA, T. M. M.; SILVA, P. de A.; BARBOSA, G. V.; HOFFMANN, H. P; CHAPOLA, R. G.; FERNANDES JUNIOR, A. R.; GAZAFFI, R. Melhoramento genético da cana-de-açúcar. Aracaju: Embrapa Tabuleiros Costeiros, 2015. 40 p. (Embrapa Tabuleiros Costeiros. Documentos, 200).| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 18. | | PEREIRA, G. S.; NUNES, E. S.; LAPERUTA, L. D. C.; BRAGA, M. F.; PENHA, H. A.; DINIZ, A. L.; MUNHOZ, C. F.; GAZAFFI, R.; GARCIA, A. A. F.; VIEIRA, M. L. C. Molecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species. Annals of Applied Biology, v. 162, n. 3, p. 347-361, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Cerrados. |
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| 19. | | PERSEGUINI, J. M. C. K.; CHIORATTO, A. F.; ZUCCHI, M. I.; COLOMBO, C. A.; CARBONELL, S. A. M.; MONDEGO, J. M. C.; GAZAFFI, R.; GARCIA, A. A. F.; CAMPOS, T. de; SOUZA, A. P. de; RUBIANO, L. B. Genetic diversity in cultivated carioca common beans based on molecular marker analysis. Genetics and Molecular Biology, Ribeirão Preto, v. 34, n. 1, p. 88-102, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Acre. |
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| 20. | | PEREIRA, G. da S.; LAPERUTA, L. D. C.; NUNES, E. S.; CHAVARRIA, L.; PASTINA, M. M.; GAZAFFI, R.; GERALDI, I. O.; GARCIA, A. A. F.; VIEIRA, M. L. C. The sweet passion fruit (Passiflora alata) crop: genetic and phenotypic parameter estimates and QTL mapping for fruit traits. Tropical Plant Biology, v. 10, n. 1, p. 18-29, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 24 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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