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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
24/05/2012 |
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Data da última atualização: |
22/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COTTA, S. R; MOTA, F. F. da; TUPINAMBÁ, G.; ISHIDA, K.; ROZENTAL, S.; SILVA, D. O. e; SILVA, A. J. R. da; BIZZO, H. R.; ALVIANO, D. S.; ALVIANO, C. S.; SELDIN, L. |
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Afiliação: |
SIMONE RAPOSO COTTA, UFRJ; FABIO FARIA DA MOTA, UFRJ; GLEISER TUPINAMBÁ, UFRJ; KELLY ISHIDA, UFRJ; SONIA ROZENTAL, UFRJ; DAVI OLIVEIRA E SILVA, UFRJ; ANTONIO JORGE RIBEIRO DA SILVA, UFRJ; HUMBERTO RIBEIRO BIZZO, CTAA; DANIELA SALES ALVIANO, UFRJ; CELUTA SALES ALVIANO, UFRJ; LUCY SELDIN, UFRJ. |
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Título: |
Antimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 28, n. 3, p. 953-962, 2012. |
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DOI: |
10.1007/s11274-011-0893-1 |
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Idioma: |
Inglês |
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Conteúdo: |
In a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used against dermatophytes. MenosIn a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used again... Mostrar Tudo |
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Palavras-Chave: |
Dermatófitos patogênicos; Paenibacillus kribbensis; Substância antimicrobiana. |
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Thesaurus Nal: |
Trichophyton rubrum. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02433naa a2200301 a 4500
001 1925383
005 2016-02-22
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1007/s11274-011-0893-1$2DOI
100 1 $aCOTTA, S. R
245 $aAntimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum.
260 $c2012
520 $aIn a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used against dermatophytes.
650 $aTrichophyton rubrum
653 $aDermatófitos patogênicos
653 $aPaenibacillus kribbensis
653 $aSubstância antimicrobiana
700 1 $aMOTA, F. F. da
700 1 $aTUPINAMBÁ, G.
700 1 $aISHIDA, K.
700 1 $aROZENTAL, S.
700 1 $aSILVA, D. O. e
700 1 $aSILVA, A. J. R. da
700 1 $aBIZZO, H. R.
700 1 $aALVIANO, D. S.
700 1 $aALVIANO, C. S.
700 1 $aSELDIN, L.
773 $tWorld Journal of Microbiology and Biotechnology$gv. 28, n. 3, p. 953-962, 2012.
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Embrapa Agroindústria de Alimentos (CTAA) |
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| 4. |  | ROSCOE, R.; VASCONCELLOS, C. A.; FURTINI NETO, A. E.; GUEDES, G. A. A. Atividade da urease em um latossolo vermelho escuro de Sete Lagoas, MG, cultivado com milho, sob diferentes métodos de preparo de solo. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 22., 1996, Manaus. Resumos expandidos... Manaus: UA/FCA/EMBRAPA/INPA, 1996. p. 541-542.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | CARVALHO, V. G.; RESENDE, A. V. de; HICKMANN, C.; FURTINI NETO, A. E.; SILVA, C. A. Disponibilidade de nitrogênio mineral decorrente da aplicação de uréias tratadas em solo com baixa umidade superficial. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2012. 1 CD-ROM. Fertbio 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 8. | | RESENDE, A. V. de; FURTINI NETO, A. E.; MUNIZ, J. A.; CURI, N.; FAQUIN, V. Crescimento inicial de espécies florestais de diferentes grupos sucessionais em resposta a doses de fósforo. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 11, p. 2071-81, nov. 1999 Título em inglês: Initial growth of forest species of different successional groups in response to phosphorus doses.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | FERNANDES, L. A.; FURTINI NETO, A. E.; FONSECA, F. C.; VALE, F. R. do. Crescimento inicial, níveis críticos de fósforo e frações fosfatadas em espécies florestais. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 6, p. 1191-98, jun. 2000. Título em inglês: Initial growing, phosphorus critical levels and phosphate fractions in forest species.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 10. | | SILVA, I. R. da; FURTINI NETO, A. E.; CURI, N.; VALE, F. R. do. Crescimento inicial de quatorze espécies florestais nativas em resposta à adubação potássica. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 32, n. 2, p. 205-212, fev. 1997. Título em inglês: Initial growth of fourteen native tree species in response to potassium fertilization.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 11. | | SANTOS, J. Z. L.; RESENDE, A. V. de; FURTINI NETO, A. E.; CORTE, E. J. Crescimento, acumulo de fosforo e fracoes fosfatadas em especies florestais nativas. In: REUNIAO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRICAO DE PLANTAS, 26.; REUNIAO BRASILEIRA SOBRE MICORRIZAS, 10.; SIMPOSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 8.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 5., 2004, Lages, SC. FertBio 2004: avaliacao das conquistas: bases para estrategias futuras. Lages: [s. n.], 2004. nao paginado.| Biblioteca(s): Embrapa Cerrados. |
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| 16. | | FERNANDES, L. A.; FAQUIN, V.; FURTINI NETO, A. E.; CURI, N. Formas de fósforo em solos de várzea e biodisponibilidade para o feijoeiro. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 3, p. 373-383, mar. 2002 Título em inglês: Phosphorus forms in lowland soils and bioavailability to bean plants.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 17. | | FURTINI NETO, A. E.; RESENDE, A. V. de; VALE, F. R. do; SILVA, I. R. Liming effects on growth of native woody species from brazilian savannah. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 5, p. 829-37, maio 1999 Título em português: Efeitos da calagem no crescimento de espécies florestais da região dos cerrados brasileiros.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 19. | | SILVA, E. de B.; NOGUEIRA, F. D.; GUIMARÃES, P. T. G.; FURTINI NETO, A. E. Resposta do cafeeiro à adubação potássica em safras de baixa e alta produção. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 36, n. 11, p. 1331-1337, nov. 2001 Título em inglês: Coffee tree response to potassium fertilization in low and high yields.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 117 | |
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