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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
29/12/2015 |
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Data da última atualização: |
25/01/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUES, A. C. F.; MONERÓ, T. de O.; FRIGHETTO, R. T. S.; ALMEIDA, E. A. de. |
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Afiliação: |
ALINE CRISTINA FERREIRA RODRIGUES, IBILCE-UNESP; TATIANA DE OLIVEIRA MONERÓ, IBILCE-UNESP; ROSA TOYOKO SHIRAISHI FRIGHETTO, CNPMA; EDUARDO ALVES DE ALMEIDA, IBILCE-UNESP. |
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Título: |
E2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Environmental Science & Pollution Research, Berlin, v. 22, n. 22, p. 17367-17374, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
In the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia. MenosIn the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolate... Mostrar Tudo |
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Palavras-Chave: |
17b-Estradiol; Biotransformation enzymes; Hidrocarboneto aromático policíclico. |
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Thesagro: |
Oreochromis niloticus; Poluição da água; Substância tóxica; Tilápia nilótica. |
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Thesaurus Nal: |
Benzopyrenes; Polycyclic aromatic hydrocarbons; Water pollution. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02768naa a2200277 a 4500
001 2032529
005 2016-01-25
008 2015 bl uuuu u00u1 u #d
100 1 $aRODRIGUES, A. C. F.
245 $aE2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations.$h[electronic resource]
260 $c2015
520 $aIn the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia.
650 $aBenzopyrenes
650 $aPolycyclic aromatic hydrocarbons
650 $aWater pollution
650 $aOreochromis niloticus
650 $aPoluição da água
650 $aSubstância tóxica
650 $aTilápia nilótica
653 $a17b-Estradiol
653 $aBiotransformation enzymes
653 $aHidrocarboneto aromático policíclico
700 1 $aMONERÓ, T. de O.
700 1 $aFRIGHETTO, R. T. S.
700 1 $aALMEIDA, E. A. de
773 $tEnvironmental Science & Pollution Research, Berlin$gv. 22, n. 22, p. 17367-17374, 2015.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 12 | |
| 3. |  | PEREIRA-CARVALHO, R. C.; BOITEUX, L. S.; NOGUEIRA, I.; FERNANDES-ACIOLI, N. A. N.; FONSECA-BOITEUX, M. E. de N. Evaluation of tomato germplasm reaction to Tomato chlorosis virus. Tropical Plant Pathology, Brasília, DF, v. 36, p. 1412, 2011. 1 CD-ROM. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 1554.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 4. | | DIANESE, E. C.; FONSECA-BOITEUX, M. E. de N.; PIÑON, M.; COSTA, A. F.; RESENDE, R. O.; BOITEUX, L. S. Application of a functional marker derived form the Sw-5 gene in accessions form tomato breeding programs conducted in the Americas, Europe, and Middle-East. Tropical Plant Pathology, Brasília, DF, v. 35, p. S211, ago. 2010. Suplemento. Resumo 07-049. Trabalho apresentado no 43. Congresso Brasileiro de Fitopatologia, 2010, Cuiabá.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 5. | | FONSECA-BOITEUX, M. E. de N.; PINÕN, M.; RODRIGUEZ, Y.; GONZÁLEZ-ARCOS, M.; ARRUABARRENA, A.; BOITEUX, L. S. Allelic diversity for a genomic segment encompassing a functional marker associated with the Sw-5 locus in wild tomato species and tomato breeding lines. In: SOLANACEAE CONFERENCE, 11., 2014, Arraial D'Ajuda. Book of abstracts. [s.l]: [s. n.], 2014. p. 162 Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 7. | | CARVALHO, S. I. C. de; BOITEUX, L. S.; FONSECA-BOITEUX, M. E. de N.; SILVA FILHO, J. G. da. Banco ativo de germoplasma de tomate mantido na Embrapa Hortaliças. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA A AMÉRICA LATINA E CARIBE, 10., 2015, Bento Gonçalves. Recursos genéticos no século 21: de Vavilov a Svalbard. Anais... [s.l.]: Sociedade Brasileira de Recursos Genéticos, 2015. p. 68. Editores técnicos: Rodrigo Cezar Franzon, Caroline Marques Castro, Alexandre Floriani Ramos, Sueli Correa Marques de Mello. SIRGEALC.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 8. | | CABRAL, C. S.; BRUNELLI, K. R.; COSTA, H.; FONSECA-BOITEUX, M. E. de N.; BOITEUX, L. S.; REIS, A. Identification of Fusarium oxysporum f. sp. lactucae race 1 as the causal agent of lettuce wilt in Brazil. Tropical Plant Pathology, Brasília, DF, v. 39, n. 3, p. 197-202, May/Jun. 2014| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | FONSECA-BOITEUX, M. E. de N.; BOITEUX, L. S.; ABREU, H.; NOGUEIRA, I.; PEREIRA-CARVALHO, R. C. Physalis angulata: a new natural host of tomato chlorosis virus in Brazil. Plant Disease, Saint Paul, v. 97, n. 5, p. 692, May 2013. Disease notes| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 11. | | BORGES, R. C. F.; SANTOS, M. D. M.; MACEDO, M. A.; MARTINS, I.; NASCIMENTO, A. G.; CAFÉ FILHO, A. C.; BOITEUX, L. S.; FONSECA-BOITEUX, M. E. de N.; INÁCIO, C. A.; MELLO, S. C. M. de. A trunk canker disease of Tectona grandis induced by Lasiodiplodia theobromae in Brazil. New Disease Reports, v. 31, p. 26, Jan./Jun 2015.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | TEODORO, A. F. P.; ALVES, R. de B. das N.; RIBEIRO, L. B.; REIS, K.; REIFSCHNEIDER, F. J. B.; FONSECA-BOITEUX, M. E. de N.; SILVA, J. P. da; AGOSTINI-COSTA, T. da S. Vitamin C content in Habanero pepper accessions (Capsicum chinense). Horticultura Brasileira, Brasilia, DF, v. 31, n. 1, p. 59-62, jan./mar. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 12 | |
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