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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
20/05/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHRISTOPHOROU, M. A.; CASTELO-BRANCO, G.; HALLEY-STOTT, R. P.; OLIVEIRA, C. S.; LOOS, R.; RADZISHEUSKAYA, A.; MOWEN, K. A.; BERTONE, P.; SILVA, J. C. R.; ZERNICKA-GOETZ, M.; MICHAEL L. NIELSEN; GURDON, J. B.; KOUZARIDES, T. |
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Afiliação: |
MARIA A. CHRISTOPHOROU, University of Cambridge; GONÇALO CASTELO-BRANCO, University of Cambridge; Karolinska Institutet, Stockholm, Sweden; RICHARD P. HALLEY-STOTT, University of Cambridge; CLARA SLADE OLIVEIRA, CNPGL; REMCO LOOS, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Cambridge; ALIAKSANDRA RADZISHEUSKAYA, University of Cambridge; KERRI A. MOWEN, Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California; PAUL BERTONE, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Cambridge; University of Cambridge; JOSÉ C. R. SILVA, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Cambridge , University of Cambridge.; MAGDALENA ZERNICKA-GOETZ, University of Cambridge; NIELSEN, M. L., University of Copenhagen,Copenhagen, Denmark; JOHN B. GURDON, University of Cambridge; TONY KOUZARIDES, University of Cambridge. |
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Título: |
Citrullination regulates pluripotency and histone H1 binding to chromatin. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Nature, v. 507, n. 7490, p. 104-108, 2014. |
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DOI: |
https://doi.org/10.1038/nature12942 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrullination is the post-translational conversion of an arginine residue within a protein to the non-coded amino acid citrulline. This modification leads to the loss of a positive charge and reduction in hydrogen-bonding ability. It is carried out by a small family of tissue-specific vertebrate enzymes called peptidylarginine deiminases (PADIs) and is associated with the development of diverse pathological states such as autoimmunity, cancer, neurodegenerative disorders, prion diseases and thrombosis. Nevertheless, the physiological functions of citrullination remain ill-defined, although citrullination of core histones has been linked to transcriptional regulation and the DNA damage response. PADI4 (also called PAD4 or PADV), the only PADI with a nuclear localization signal, was previously shown to act in myeloid cells where it mediates profound chromatin decondensation during the innate immune response to infection. Here we show that the expression and enzymatic activity of Padi4 are also induced under conditions of ground-state pluripotency and during reprogramming in mouse. Padi4 is part of the pluripotency transcriptional network, binding to regulatory elements of key stem-cell genes and activating their expression. Its inhibition lowers the percentage of pluripotent cells in the early mouse embryo and significantly reduces reprogramming efficiency. Using an unbiased proteomic approach we identify linker histone H1 variants, which are involved in the generation of compact chromatin, as novel PADI4 substrates. Citrullination of a single arginine residue within the DNA-binding site of H1 results in its displacement from chromatin and global chromatin decondensation. Together, these results uncover a role for citrullination in the regulation of pluripotency and provide new mechanistic insights into how citrullination regulates chromatin compaction. MenosCitrullination is the post-translational conversion of an arginine residue within a protein to the non-coded amino acid citrulline. This modification leads to the loss of a positive charge and reduction in hydrogen-bonding ability. It is carried out by a small family of tissue-specific vertebrate enzymes called peptidylarginine deiminases (PADIs) and is associated with the development of diverse pathological states such as autoimmunity, cancer, neurodegenerative disorders, prion diseases and thrombosis. Nevertheless, the physiological functions of citrullination remain ill-defined, although citrullination of core histones has been linked to transcriptional regulation and the DNA damage response. PADI4 (also called PAD4 or PADV), the only PADI with a nuclear localization signal, was previously shown to act in myeloid cells where it mediates profound chromatin decondensation during the innate immune response to infection. Here we show that the expression and enzymatic activity of Padi4 are also induced under conditions of ground-state pluripotency and during reprogramming in mouse. Padi4 is part of the pluripotency transcriptional network, binding to regulatory elements of key stem-cell genes and activating their expression. Its inhibition lowers the percentage of pluripotent cells in the early mouse embryo and significantly reduces reprogramming efficiency. Using an unbiased proteomic approach we identify linker histone H1 variants, which are involved in the generation of com... Mostrar Tudo |
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Palavras-Chave: |
Histone post-translational modifications; Reprogramming. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 10 | |
| 1. |  | PEREIRA-CARVALHO, R. C.; DIAZ-PENDÓN, J. A.; FERNANDÉZ-MUÑOZ, R.; BOITEUX, L. S.; MORIONES, E.; RESENDE, R. O. Estudo do mecanismo de resistência a begomovirus na linhagem de tomate 'TX-468' e seu efeito na dispersão do Tomato yellow leaf curl virus por Bemisia tabaci. Tropical Plant Pathology, Brasília, DF, v. 33, p. S294, ago. 2008. Suplemento. Resumo VIR 039. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual Meeting of the Brazilian Phytopathological Society, Belo Horizonte, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 2. | | GARCIA-CANO, E.; RESENDE, R. O.; BOITEUX, L. S.; GIORDANO, L. de B.; FERNÁNDEZ-MUÑOZ, R.; MORIONES, E. Phenotypic expression, stability, and inheritance of a recessive resistance to monopartite begomoviruses associated with tomato yellow leaf curl disease in tomato. Phytopathology, Saint Paul, v. 98, n. 5, p. 618-627, jan. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Hortaliças. |
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| 3. | | PEREIRA-CARVALHO, R. C.; BOITEUX, L. S.; FONSECA, M. E. N.; MORIONES, E.; FERNANDEZ-MUÑOZ, R.; DIAZ-PENDON, J. M.; RESENDE, R. O. Aspectos epidemiológicos da dispersão de tomato Yellow leaf curl virus-Israel (TYLCV-IL) por Bemisia tabaci biótipo Q. Tropical Plant Pathology, Brasília, DF, v. 34, p. 40, ago. 2009. Suplemento. Resumo da palestra apresentada no 42. Congresso Brasileiro de Fitopatologia, Rio de Janeiro.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 4. | | PEREIRA-CARVALHO, R. C.; BOITEUX, L. S.; FONSECA, M. E. N.; DÍAZ-PENDÓN, J. A.; MORIONES, E.; FERNÁNDEZ-MUÑOZ, R.; CHARCHAR, J. M.; RESENDE, R. O. Multiple resistance to meloidogyne spp. and to bipartite and monopartite begomovirus spp. in wild Solanum (Lycopersicon) accessions. Plant Disease, v. 94, n. 2, p. 179-185, Feb. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 5. | | BOITEUX, L. S.; PEREIRA-CARVALHO, R. C.; INOUE-NAGATA, A. K.; FONSECA, M. E. N.; RESENDE, R. de O.; FERNÁNDEZ-MUÑOZ, R. Reação de acessos de tomateiro portando o gene Ty-2 (introgredido de Solanum habrochaites f. glabratum) a um isolado de begomovírus de genoma bipartido. In: CONGRESSO BRASILEIRO DE OLERICULTURA, 47.; SIMPÓSIO BRASILEIRO SOBRE CUCURBITÁCEAS, 4., 2007, Porto Seguro. Resgatando e valorizando as hortaliças subutilizadas. Porto Seguro: Associação Brasileira de Horticultura, 2007. CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Hortaliças. |
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| 6. | | BOITEUX, L. S.; PEREIRA-CARVALHO, R. C.; INOUE-NAGATA, A. K.; FONSECA, M. E. N.; RESENDE, R. de O.; FERNÁNDEZ-MUÑOZ, R. Reação de acessos de tomateiro portando o gene Ty-2 (introgredido de Solanum habrochaites f. glabratum) a um isolado de begomovírus de genoma bipartido. Horticultura Brasileira, Brasília, DF, v. 25, n. 1, p. s97, ago. 2007. Suplemento. Resumo 521. Trabalho apresentado no 47. Congresso Brasileiro de Olericultura, 4. Simpósio Brasileiro sobre Cucurbitáceas, 2007. Porto Seguro.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 7. | | PEREIRA-CARVALHO, R. C.; DIAZ-PENDÓN, J. A.; FONSECA, M. E. de N.; BOITEUX, L. S.; FERNANDEZ-MUÑOZ, R.; MORIONES, E.; RESENDE, R. O. Recessive resistance derived from tomato cv. Tyking-Limits drastically the spread of tomato yellow leaf curl virus. Viruses, v. 7, n. 5, p. 2518-2533, May 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 8. | | GONZÁLEZ-ARCOS, M.; FONSECA, M. E. N.; ARRUABARENA, A.; LIMA, M. F.; MICHEREFF FILHO, M.; MORIONES, E.; FERNÁNDEZ-MUÑOZ, R.; BOITEUX, L. S. Identification of genetic sources with attenuated tomato chlorosis virus-induced symptoms in Solanum (section Lycopersicon) germplasm. Euphytica, v. 214, n. 10, p. 1-16, Oct. 2018. Article 178.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 9. | | PEREIRA-CARVALHO, R. C.; BOITEUX, L. S.; FONSECA, M. E. N.; MORIONES, E.; FERNANDEZ-MUÑOZ, R.; DIAZ-PENDÓN, J.A.; CHARCHAR, J. M.; RESENDE, R. O. Identification in solanum (section lycopersicon) species of genetic sources combining resistance to root-knot nematodes, monopartite and bipartite begomovirus. Virus Reviews & Research, São Paulo, v. 14, p. 247-248, nov. 2009. Suplemento 1. Resumo.Trabalho apresentado no 20. National Meeting of Virology, 2009, Brasília, Distrito Federal, Brasil.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | SILVA, K. F. A. S.; MICHEREFF FILHO, M.; FONSECA, M. E. N.; SILVA FILHO, J. G.; TEIXEIRA, A. C. A.; MOITA, A. W.; TORRES, J. B.; FERNANDEZ-MUNOZ, R.; BOITEUX, L. S. Resistance to Bemisia tabaci biotype B of Solanum pimpinellifolium is associated with higher densities of type IV glandular trichomes and acylsugar accumulation. Entomologia Experimentalis et Applicata, v. 151, p. 2018-230, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 10 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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