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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
27/03/2023 |
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Data da última atualização: |
12/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. |
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Afiliação: |
PAULA CRISTINA DA SILVA ANGELO, CNPCa; L. F. P. PEREIRA, IDR - PARANÁ; G. H. SERA, IDR - PARANÁ; T. SERA, IDR - PARANÁ. |
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Título: |
Coffee microspore cultivation to attain doubled-haploid plantlets. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Acta Horticulturae, v. 31, n. 1359, p. 123-130, 2023. |
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DOI: |
10.17660/ActaHortic.2023.1359.14 |
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Idioma: |
Inglês |
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Notas: |
Apresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. |
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Conteúdo: |
Introgression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation. MenosIntrogression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control ant... Mostrar Tudo |
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Thesagro: |
Embriogénese; Pólen; Rubiaceae. |
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Thesaurus Nal: |
Embryogenesis; Pollen. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02752naa a2200241 a 4500
001 2152776
005 2023-04-12
008 2023 bl uuuu u00u1 u #d
024 7 $a10.17660/ActaHortic.2023.1359.14$2DOI
100 1 $aANGELO, P. C. da S.
245 $aCoffee microspore cultivation to attain doubled-haploid plantlets.$h[electronic resource]
260 $c2023
500 $aApresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.
520 $aIntrogression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation.
650 $aEmbryogenesis
650 $aPollen
650 $aEmbriogénese
650 $aPólen
650 $aRubiaceae
700 1 $aPEREIRA, L. F. P.
700 1 $aSERA, G. H.
700 1 $aSETA, T.
773 $tActa Horticulturae$gv. 31, n. 1359, p. 123-130, 2023.
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| 15. |  | MIRANDA, C. H. B.; VERZIGNASSI, J. R.; FERNANDES, C. D. Biological nitrogen fixation in the tropical forage legume Stylo. In: ADSA-PSA-AMPA-ASAS JOINT ANNUAL MEETING, 2010, Denver. [Abstracts]. Publicado em: Journal of Animal Science, Champaign, v. 88, E-Supplement 2/J; Journal of Dairy Science, Champaign, v. 93, E-Supplement 1.; Journal of Poultry Science, v.89, E- Supplement 1. Forages and Pastures: Forage Quality. Trabalho: T128. p. 346| Tipo: Resumo em Anais de Congresso |
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| 16. | | FERNANDES, C. D.; MARCHI, C. E.; BATISTA, M. V. Fitonematóides em sementes de forrageiras tropicais irradiadas em cobalto. Tropical Plant Pathology, Brasília, DF, v. 34, p. S230, ago. 2009. Suplemento, ref. 773. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia; Annual Meeting of the Brazilain Phytopathological Society, Rio de Janeiro, ago. 2009.| Tipo: Resumo em Anais de Congresso |
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| 18. | | FERNANDES, C. D.; VERZIGNASSI, J. R.; AZAMBUJA, D. G. Reacao de acessos de Stylosanthes spp. a antracnose. Fitopatologia Brasileira, Brasilia, v. 26, p. 422, ago. 2001. Suplemento, ref. 588. Edicao de palestras e resumos do XXXIV Congresso Brasileiro de Fitopatologia, Sao Pedro, SP, ago. 2001. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 19. | | FERNANDES, A. T. F.; FERNANDES, C. D.; CHAKRABORTY, S. Reacao de acessos de Stylosanthes spp. a antracnose. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 32., 1999, Curitiba. Resumos. Fitopatologia Brasileira, Brasilia, v.24, supl., p.283, ago. 1999. Resumo 228.| Biblioteca(s): Embrapa Gado de Corte. |
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