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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/02/2008 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
AGUIAR-PERECIN, M. L. R.; MONDIN, M.; SANTOS-SEREJO, J. A. dos; VIDA, A. C. F.; MOLINA, S. C. M. |
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Afiliação: |
Margarida L. R. Aguiar-Perecin, USP; Mateus Mondin, USP; Janay Almeida dos Santos-Serejo, CNPMF; Ana C. F. Vida, USP; Silvia C. M. Molina, USP. |
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Título: |
Analysis of karyotype variability of tropical maize inbred lines and hybrids using FISH of tandemly repeated DNA sequences to identify somatic chromosomes. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: Chomosome Research, Netherlands, v. 15, n. 2, p. 41 jul. 2007. Supplement. P.088 |
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ISSN: |
0967-3849 |
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Idioma: |
Inglês |
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Notas: |
Edição do 16 INTERNATIONAL CHROMOSOME CONFERENCE, Abstracts...Amsterdam, ago. 2007. Editado por Hans de Jong, Hans Tanke and Paul Fransz. DOI: 10.1007/s10577 ISSN 0967-3849 em papel |
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Conteúdo: |
The analysis of pachytene chromosomes has greatly contributed to maize cytogenetics. Recently, FISH procedures using repeated DNA sequences have been described and improved the identification of somatic chromosomes. Due to the polymorphism of knobs and repetitive DNA sequences, karyotype diversity is observed among varieties. Mitotic chromosome analysis is valuable for the screening of several individuals, and studies of evolution. We investigaded root-tip chromosomes of inbred lines and hybrids, derived from a tropical variety (Cateto races), using the following sequences as probes: subtelomeric 4-12-1 clone (PNAS 101, 13555), knob 180-bp sequence, 5S rDNA, centromeric satellite 4 (Cent 4). Pachytene chromosomes and C-banded mitotic metaphases were previously analysed, and except for chromosomes 2, 4 and 5, most chromosomes were distinguished by their length, arm ratio or presence of knobs. We identified chromosomes 2 and 4 using probes of 5S rDNA (2L) and Cent4, respectively, but their arm ratios were atypical. Signals of the 180-bp sequence were correspondent to knobs detected by C-banding, and additional small signals were found at 1S, 6S and 9S, located at arm tips, a feature also found in other varieties. The subtelomeric sequence was observed at 1S, 2S, 4SL, 5S, 8L. One line was crossed with KYS, an inbred extensively investigated, and the following observations shoul be emphasized: differences in the subtelomeric sequence signals; homologues (with different arm ratios) of chromosomes 2 and 4 formed normal bivalents at pachtytene. This aspect apparently provides evidence for epigenetic location of centromeres, a finding that has been observed in many organisms. MenosThe analysis of pachytene chromosomes has greatly contributed to maize cytogenetics. Recently, FISH procedures using repeated DNA sequences have been described and improved the identification of somatic chromosomes. Due to the polymorphism of knobs and repetitive DNA sequences, karyotype diversity is observed among varieties. Mitotic chromosome analysis is valuable for the screening of several individuals, and studies of evolution. We investigaded root-tip chromosomes of inbred lines and hybrids, derived from a tropical variety (Cateto races), using the following sequences as probes: subtelomeric 4-12-1 clone (PNAS 101, 13555), knob 180-bp sequence, 5S rDNA, centromeric satellite 4 (Cent 4). Pachytene chromosomes and C-banded mitotic metaphases were previously analysed, and except for chromosomes 2, 4 and 5, most chromosomes were distinguished by their length, arm ratio or presence of knobs. We identified chromosomes 2 and 4 using probes of 5S rDNA (2L) and Cent4, respectively, but their arm ratios were atypical. Signals of the 180-bp sequence were correspondent to knobs detected by C-banding, and additional small signals were found at 1S, 6S and 9S, located at arm tips, a feature also found in other varieties. The subtelomeric sequence was observed at 1S, 2S, 4SL, 5S, 8L. One line was crossed with KYS, an inbred extensively investigated, and the following observations shoul be emphasized: differences in the subtelomeric sequence signals; homologues (with different arm ratio... Mostrar Tudo |
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Palavras-Chave: |
Maize cytogenetics. |
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Thesaurus Nal: |
chromosomes. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02599nam a2200205 a 4500
001 1654675
005 2023-07-24
008 2007 bl uuuu u00u1 u #d
022 $a0967-3849
100 1 $aAGUIAR-PERECIN, M. L. R.
245 $aAnalysis of karyotype variability of tropical maize inbred lines and hybrids using FISH of tandemly repeated DNA sequences to identify somatic chromosomes.$h[electronic resource]
260 $aIn: Chomosome Research, Netherlands, v. 15, n. 2, p. 41 jul. 2007. Supplement. P.088$c2007
500 $aEdição do 16 INTERNATIONAL CHROMOSOME CONFERENCE, Abstracts...Amsterdam, ago. 2007. Editado por Hans de Jong, Hans Tanke and Paul Fransz. DOI: 10.1007/s10577 ISSN 0967-3849 em papel
520 $aThe analysis of pachytene chromosomes has greatly contributed to maize cytogenetics. Recently, FISH procedures using repeated DNA sequences have been described and improved the identification of somatic chromosomes. Due to the polymorphism of knobs and repetitive DNA sequences, karyotype diversity is observed among varieties. Mitotic chromosome analysis is valuable for the screening of several individuals, and studies of evolution. We investigaded root-tip chromosomes of inbred lines and hybrids, derived from a tropical variety (Cateto races), using the following sequences as probes: subtelomeric 4-12-1 clone (PNAS 101, 13555), knob 180-bp sequence, 5S rDNA, centromeric satellite 4 (Cent 4). Pachytene chromosomes and C-banded mitotic metaphases were previously analysed, and except for chromosomes 2, 4 and 5, most chromosomes were distinguished by their length, arm ratio or presence of knobs. We identified chromosomes 2 and 4 using probes of 5S rDNA (2L) and Cent4, respectively, but their arm ratios were atypical. Signals of the 180-bp sequence were correspondent to knobs detected by C-banding, and additional small signals were found at 1S, 6S and 9S, located at arm tips, a feature also found in other varieties. The subtelomeric sequence was observed at 1S, 2S, 4SL, 5S, 8L. One line was crossed with KYS, an inbred extensively investigated, and the following observations shoul be emphasized: differences in the subtelomeric sequence signals; homologues (with different arm ratios) of chromosomes 2 and 4 formed normal bivalents at pachtytene. This aspect apparently provides evidence for epigenetic location of centromeres, a finding that has been observed in many organisms.
650 $achromosomes
653 $aMaize cytogenetics
700 1 $aMONDIN, M.
700 1 $aSANTOS-SEREJO, J. A. dos
700 1 $aVIDA, A. C. F.
700 1 $aMOLINA, S. C. M.
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| 17. |  | FALEIRO, F. G.; GAMA, L. C.; FARIAS NETO, A. L. de; SOUSA, E. dos S. de. O Simpósio Nacional sobre o Cerrado e o Simpósio Internacional sobre Savanas Tropicais. In: FALEIRO, F. G.; FARIAS NETO, A. L. de (Ed.). Savanas: desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais. Planaltina, DF: Embrapa Cerrados, 2008. cap. 1, p. 33-46.| Tipo: Capítulo em Livro Técnico-Científico |
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| 18. | | FARIAS NETO, A. L. de; RIBEIRO JÚNIOR, W. Q.; FALEIRO, F. G. A Sociedade Brasileira de Melhoramento de Plantas - Regional DF. In: FALEIRO, F. G.; FARIAS NETO, A. L. de; RIBEIRO JÚNIOR, W. Q. (Ed.). Pré-melhoramento, melhoramento e pós-melhoramento: estratégias e desafios. Planaltina, DF: Embrapa Cerrados, 2008. cap. 1, p. 17-28.| Tipo: Capítulo em Livro Técnico-Científico | Circulação/Nível: -- - -- |
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