| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
28/08/2018 |
|
Data da última atualização: |
24/01/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FERREIRA, J. R.; MINELLA, E.; DELATORRE, C. A.; DELHAIZE, E.; RYAN, P. R.; PEREIRA, J. F. |
|
Afiliação: |
Jéssica Rosset Ferreira, UFRGS; EUCLYDES MINELLA, CNPT; Carla Andréa Delatorre, UFRGS; Emmanuel Delhaize, CSIRO; Peter R. Ryan, CSIRO; JORGE FERNANDO PEREIRA, CNPGL. |
|
Título: |
Conventional and transgenic strategies to enhance the acid soil tolerance of barley. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Molecular Breeding, v. 38, n. 12, 2018. |
|
Páginas: |
11 p. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract The aluminum (Al3+) tolerance of barley cultivars predominately from Brazil was compared to that of cultivars from other countries, wild barley accessions, and a transgenic line (L5) over-expressing TaALMT1, the major Al3+ tolerance gene from wheat. After screening conventional germplasm for Al3+ tolerance in hydroponics, 18 genotypes were further characterized in a short-term soil experiment. Among the Brazilian cultivars, Antarctica 01 and BRS Mariana showed the greatest relative root length (RRL) in acid soil. However, these cultivars were significantly less tolerant than the foreign cultivars Dayton (USA) and Murasakimochi (Japan) and the transgenic line L5 which out-performed all conventional genotypes. In long-term growth trials, the transgenic line produced the greatest relative root and relative shoot dry weight. Relative grain yield was greatest in the transgenic line and Dayton. All genotypes were also scored for two genetic markers linked to HvAACT1, the major Al3+ tolerance gene in barley. One marker detects a 1-kb insertion in the promoter that increases gene expression and leads to increased Al3+- activated citrate efflux from root apices. The other marker detects a 21-bp indel downstream of the coding region. The 1-kb insertion was only detected in Dayton and Murasakimochi that were the best performing cultivars among the non-transgenic germplasm. Interestingly, the Brazilian cultivars with an intermediate level of tolerance, Antarctica 01 and BRS Mariana, lacked the 1-kb insertion but had enhanced HvAACT1 expression compared to an Al3+-sensitive cultivar. No clear correlation was observed between Al3+ tolerance and the 21-bp indel marker in the short-term soil trials. We conclude that improved Al3+ tolerance in barley could be achieved by combining the best allele of HvAACT1 along with TaALMT1 as a transgene. MenosAbstract The aluminum (Al3+) tolerance of barley cultivars predominately from Brazil was compared to that of cultivars from other countries, wild barley accessions, and a transgenic line (L5) over-expressing TaALMT1, the major Al3+ tolerance gene from wheat. After screening conventional germplasm for Al3+ tolerance in hydroponics, 18 genotypes were further characterized in a short-term soil experiment. Among the Brazilian cultivars, Antarctica 01 and BRS Mariana showed the greatest relative root length (RRL) in acid soil. However, these cultivars were significantly less tolerant than the foreign cultivars Dayton (USA) and Murasakimochi (Japan) and the transgenic line L5 which out-performed all conventional genotypes. In long-term growth trials, the transgenic line produced the greatest relative root and relative shoot dry weight. Relative grain yield was greatest in the transgenic line and Dayton. All genotypes were also scored for two genetic markers linked to HvAACT1, the major Al3+ tolerance gene in barley. One marker detects a 1-kb insertion in the promoter that increases gene expression and leads to increased Al3+- activated citrate efflux from root apices. The other marker detects a 21-bp indel downstream of the coding region. The 1-kb insertion was only detected in Dayton and Murasakimochi that were the best performing cultivars among the non-transgenic germplasm. Interestingly, the Brazilian cultivars with an intermediate level of tolerance, Antarctica 01 and BRS Mar... Mostrar Tudo |
|
Palavras-Chave: |
Aluminum tolerance; Citrate transporter; HvAACT1; Malatetransporter; TaALMT1. |
|
Thesaurus Nal: |
Hydroponics. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02589naa a2200265 a 4500
001 2094656
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
100 1 $aFERREIRA, J. R.
245 $aConventional and transgenic strategies to enhance the acid soil tolerance of barley.$h[electronic resource]
260 $c2018
300 $a11 p.
520 $aAbstract The aluminum (Al3+) tolerance of barley cultivars predominately from Brazil was compared to that of cultivars from other countries, wild barley accessions, and a transgenic line (L5) over-expressing TaALMT1, the major Al3+ tolerance gene from wheat. After screening conventional germplasm for Al3+ tolerance in hydroponics, 18 genotypes were further characterized in a short-term soil experiment. Among the Brazilian cultivars, Antarctica 01 and BRS Mariana showed the greatest relative root length (RRL) in acid soil. However, these cultivars were significantly less tolerant than the foreign cultivars Dayton (USA) and Murasakimochi (Japan) and the transgenic line L5 which out-performed all conventional genotypes. In long-term growth trials, the transgenic line produced the greatest relative root and relative shoot dry weight. Relative grain yield was greatest in the transgenic line and Dayton. All genotypes were also scored for two genetic markers linked to HvAACT1, the major Al3+ tolerance gene in barley. One marker detects a 1-kb insertion in the promoter that increases gene expression and leads to increased Al3+- activated citrate efflux from root apices. The other marker detects a 21-bp indel downstream of the coding region. The 1-kb insertion was only detected in Dayton and Murasakimochi that were the best performing cultivars among the non-transgenic germplasm. Interestingly, the Brazilian cultivars with an intermediate level of tolerance, Antarctica 01 and BRS Mariana, lacked the 1-kb insertion but had enhanced HvAACT1 expression compared to an Al3+-sensitive cultivar. No clear correlation was observed between Al3+ tolerance and the 21-bp indel marker in the short-term soil trials. We conclude that improved Al3+ tolerance in barley could be achieved by combining the best allele of HvAACT1 along with TaALMT1 as a transgene.
650 $aHydroponics
653 $aAluminum tolerance
653 $aCitrate transporter
653 $aHvAACT1
653 $aMalatetransporter
653 $aTaALMT1
700 1 $aMINELLA, E.
700 1 $aDELATORRE, C. A.
700 1 $aDELHAIZE, E.
700 1 $aRYAN, P. R.
700 1 $aPEREIRA, J. F.
773 $tMolecular Breeding$gv. 38, n. 12, 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 101 | |
| 6. |  | TREVISOL, I. M.; ZANI, G. da S.; ESTEVES, P. A.; CARON, L. Adenovírus na avicultura do Brasil. Avicultura Industrial, Itu, ed. 1335, ano 115, n. 5, p. 22-29, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Suínos e Aves. |
|    |
| 7. | | VIANCELLI, A.; KUNZ, A.; ESTEVES, P. A.; NISHIYAMA, T.; FUJII, T.; VANOTTI, M.; ANTONIO, R. V. Analysis of bacterial community from a sludge reactor with anammox activity. In: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS DE ANIMAIS, 1., 2009, Florianópolis. Anais [das] palestras. Concórdia: Embrapa Suínos e Aves, 2009. v. 2 p. 502-505. Projeto/Plano de Ação: 02.03.21.800-007. I SIGERA.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 8. | | ZANELLA, J. R. C.; MORÉS, N.; SCHAEFER, R.; ESTEVES, P. A.; BRENTANO, L. Clonagem, expressão de antígenos recombinantes do vírus da doença de Aujeszky dos suínos: desenvolvimento e validação de teste de diagnóstico diferencial para monitoria em área livre. In: BERTOL, T. M.; COLDEBELLA, A. (Org.). Relatório dos projetos concluídos 2008. Concórdia: Embrapa Suínos e Aves, 2008. (Embrapa Suínos e Aves. Documentos, 129). Projeto/Plano de Ação: 03.03.230.01.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 13. | | SGARBOSSA, M. D.; BAUER, C.; COLDEBELLA, A.; CARON, L.; DUARTE, S. C.; ESTEVES, P. A. Fatores de risco do pré-abate e abate sobre as condenações por contaminação de carcaças de frango de corte ao abate. CONFERÊNCIA FACTA-WPSA BRASIL, 2018, Campinas, SP. Avicultura 4.0: otimização dos processos de produção avícola. Campinas: FACTA, 2018. Prêmio Lamas 2018.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 15. | | OKINO, C. H.; BRENTANO, L.; MORES, M. A. Z.; ESTEVES, P. A.; TREVISOL, I. M. Differential profile of local inflammatory response after challenge with Brazilian field isolates of avian infectious bronchitis virus. In: PSA ANNUAL MEETING, 2014, Corpus Christi. Abstracts... Champaign: Poultry Science Association, 2014. p. 111. Publicado em: Poultry Science, v. 93, E-Suppl. 1, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 18. | | GRITTI, D.; TREVISOL, I. M.; KLEIN, T. A. P.; ESTEVES, P. A.; SCHAEFER, R. Utilização da técnica de RT-PCR para a detecção do vírus da doença de Gumboro em amostras congeladas. In: SEMINÁRIO DE DESENVOLVIMENTO DA PESQUISA - SEDEPE, 12; JORNADA DE INICIAÇÃO CIENTÍFICA, 2., 2008, Concórdia. Evolução e diversidade: anais... Concórdia: UnC, 2008. Projeto/Plano de Ação: 02.05.11.800-03. Publicado em CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 20. | | TREVISOL, I. M.; CARON, L.; ESTEVES, P. A.; MORES, M. A. Z. Principais causas infecciosas recentes aerossaculite em frangos de corte. Avicultura Industrial, Itu, ed. 1325, ano 114, n. 02, p. 12-18, 2023.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| Registros recuperados : 101 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
