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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/03/2017 |
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Data da última atualização: |
14/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, S. O. D. de; SAYD, R. M.; BALZON, T. A.; SCHERWINSKI PEREIRA, J. E. |
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Afiliação: |
Sharrine Omari Domingues de Oliveira, Universidade Federal de Viçosa; Ricardo Meneses Sayd, UnB; Talita Aparecida Balzon, Universidade Federal do Amazonas; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
A new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Scientia Horticulturae, v. 161, p. 204-209, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
We have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results indicate the establishment of a new procedure for vanilla micropropagation through DPS, with a high production of plantlets at the end of the process and great potential for agriculture application. In addition, the presented system is more economic than the single-phase during the subcultures. MenosWe have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results ind... Mostrar Tudo |
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Palavras-Chave: |
Shoot proliferation. |
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Thesagro: |
Micropropagação. |
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Thesaurus Nal: |
micropropagation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02408naa a2200193 a 4500
001 2067485
005 2024-06-14
008 2013 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, S. O. D. de
245 $aA new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system.$h[electronic resource]
260 $c2013
520 $aWe have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results indicate the establishment of a new procedure for vanilla micropropagation through DPS, with a high production of plantlets at the end of the process and great potential for agriculture application. In addition, the presented system is more economic than the single-phase during the subcultures.
650 $amicropropagation
650 $aMicropropagação
653 $aShoot proliferation
700 1 $aSAYD, R. M.
700 1 $aBALZON, T. A.
700 1 $aSCHERWINSKI PEREIRA, J. E.
773 $tScientia Horticulturae$gv. 161, p. 204-209, 2013.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 14 | |
| 1. |  | MARTINS, K. B.; ZAFALON, L. F.; VERÍSSIMO, C. J.; ESTEVES, S. N.; DIAS, W. A. F. Etiologia infecciosa da mastite ovina em rebanhos do Estado de São Paulo. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 17.; JORNADA CIENTÍFICA DA UFSCar, 8., 2009, São Carlos, SP. Anais... São Carlos: UFSCar, 2009. p. 233.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 5. | | DIAS, W. A. F.; ZAFALON, L. F.; MARTINS, K. B.; ESTEVES, S. N. Correlação da contagem de células somáticas com o ganho de peso diário em ovinos de rebanhos de corte. In: JORNADA CIENTÍFICA-EMBRAPA SÃO CARLOS, 2009, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste: Embrapa Instrumentação Agropecuária, 2009. Editado por Luiz Francisco Zafalon, Simone Cristina Méo Niciura. (Embrapa Pecuária Sudeste. Documento 90)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | DIAS, W. A. F.; ZAFALON, L. F.; MARTINS, K. B.; ESTEVES, S. N.; VERÍSSIMO, C. J. Relação da mastite subclínica com o ganho de peso de cordeiros em rebanhos de corte. In: JORNADA CIENTÍFICA-EMBRAPA SÃO CARLOS, 2009, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste: Embrapa Instrumentação Agropecuária, 2009. Editado por Luiz Francisco Zafalon, Simone Cristina Méo Niciura. (Embrapa Pecuária Sudeste. Documento 90)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 8. | | MARTINS, K. B.; DIAS, W. A. F.; ZAFALON, L. F.; VERÍSSIMO, C. J.; ESTEVES, S. N. Perfis de resistência de estafilococos coagulase negativa isolados da mastite ovina. In:CONGRESSO DE INICIAÇÃO CIENTÍFICA DO UNICEP, 12., 2009, São Carlos, SP. Anais... São Carlos, SP: UNICEP, 2009. p. 15.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 9. | | ZAFALON, L. F.; ESTEVES, S. N.; MACHADO, R.; MARTINS, K. B.; DIAS, W. A. F. Qualidade do leite ovino e sua influência no desenvolvimento de cordeiros. São Carlos, SP: Embrapa Pecuária Sudeste, 2010. , 2010. São Carlos, SP: Embrapa Instrumentação Agrícola, 2010. 20 p. (Embrapa Pecuária Sudeste. Boletim de Pesquisa e Desenvolvimento, 28).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 13. | | ZAFALON, L. F.; MARTINS, K. B.; DIAS, W. A. F.; VERÍSSIMO, C. J.; ESTEVES, S. N.; VESCHI, J. L. A. Efeitos da mastite subclínica infecciosa sobre o desempenho de cordeiros na raça Santa Inês. Revista Brasileira de Medicina Veterinária, v. 35, n. 4, p. 299-305, out./dez. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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| 14. | | ZAFALON, L. F.; VERÍSSIMO, C. J.; ESTEVES, S. N.; MARTINS, K. B.; DIAS, W. A. F.; VESCHI, J. L. A. Mastite subclínica em ovelhas: características físico-químicas e celulares do leite. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 4., 2010, Florianópolis. Anais... Florianópolis: CBQL, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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| Registros recuperados : 14 | |
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