| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
04/12/2017 |
|
Data da última atualização: |
04/12/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ERGUN; SOUZA, F. V. D. |
|
Afiliação: |
ERGUN KAYA, Mugla Sitki Kocman University; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
|
Título: |
Comparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, v.53, Issue 4,p 410?417,| August 2017. |
|
ISSN: |
1475-2689 |
|
DOI: |
10.1007/s11627-017-9837-2 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Conservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stability for all cryopreserved varieties. MenosConservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stab... Mostrar Tudo |
|
Thesagro: |
Criopreservação. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02196naa a2200169 a 4500
001 2081361
005 2017-12-04
008 2017 bl uuuu u00u1 u #d
022 $a1475-2689
024 7 $a10.1007/s11627-017-9837-2$2DOI
100 1 $aERGUN
245 $aComparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers.$h[electronic resource]
260 $c2017
520 $aConservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stability for all cryopreserved varieties.
650 $aCriopreservação
700 1 $aSOUZA, F. V. D.
773 $tIn Vitro Cellular & Developmental Biology - Plant$gv.53, Issue 4,p 410?417,| August 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 10 | |
| 1. |  | DIAS, D. A.; POLO, L. R. T.; LAZZARI, F.; SILVA, G. J. da; SCHUSTER, I. Genome-wide association for mapping QTLs linked to protein and oil contents in soybean. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 52, n. 10, p. 896-904, out. 2017. Título em português: Associação genômica ampla para mapeamento de QTLs ligados a conteúdos de proteína e óleo em soja.| Biblioteca(s): Embrapa Unidades Centrais. |
|    |
| 3. | | FERREIRA, E. A. B.; PINTO, A. de S.; BUSTAMANTE, M. M. da C.; DIAS, D. A. S. S.; GONZALEZ, C. R. Alterações no d13C da matéria orgânica particulada do solo em um cerrado sentido restrito após proteção contra fogo e pastejo. In: CONGRESSO INTERNACIONAL DE ECOLOGIA, 1.; CONGRESSO DE ECOLOGIA DO BRASIL, 11., 2013, Porto Seguro, BA. Anais... Porto Seguro: SEB, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
| |
| 4. | | DIAS, D. A.; FERNANDES, I. A.; MACHADO, E. P.; PEDOTT, L.; MORAES, M. C. B.; BORGES, M.; STEFFENS, J.; STEFFENS, C. Nanosensors for detecting volatile compounds in pest management: a focus on agricultural sustainability. ACS Agricultural Science and Technology, v. 4, p. 1250-1259, 2024. Na publicação: Maria Carolina Blassioli-Moraes.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 5. | | DIAS, D. A.; FERNANDES, I. A.; MACHADO, E. P.; FONSECA, E. A. da; BOFF, A.; MORAES, M. C. B.; STEFFENS, J.; STEFFENS, C. Nanosensor technology for monitoring Diceraeus melacanthus in agricultural systems. ACS Agricultural Science & Technology, v. 6, n. 1, p. 244-255, 2026.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 6. | | SIMÕES, W. L.; COELHO, E. F.; MARTINEZ, M. A.; COELHO FILHO, M. A.; COSTA, E. L.; VEIMROBER JÚNIOR, L. A. dos A.; DIAS, D. A. F. Distribuição bidimensional das diferentes classes do sistema radicular da lima ácida ?tahiti? sob diferentes sistemas de irrigação por microaspersão. In: CONGRESSO NACIONAL DE IRRIGAÇÃO E DRENAGEM, 17., 2007. Mossoró. Agricultura no semi-árido. Mossoró: [s.n.], 2007.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 7. | | MARTINAZZO, J.; BALLEN, S. C.; FERNANDES, I. A.; UTTEICH, R. V.; DIAS, D. A.; STEFFENS, J.; STEFFENS, C.; MORAES, M. C. B.; BORGES, M. Detecção de feromônio de Euschistus heros em septo de borracha com nanossensor de cantilever. In: CONGRESSO DIGITAL DE NANOBIOTECNOLOGIA E BIOENGENHARIA, 3., 2022, Brasília. [Anais...]. Brasília: Embrapa Recursos Genéticos e Biotecnologia. 2022. Na publicação: Maria Carolina Blassioli-Moraes.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 9. | | MARTINAZZO, J.; UTTEICH, R. V.; FERNANDES, I. A.; DIAS, D. A.; LAUMANN, R. A.; MORAES, M. C. B.; BORGES, M.; STEFFENS, J.; STEFFENS, C. Detection of Euschistus heros and Diceraeus melacanthus volatile semiochemicals using PANI.Ag cantilever nanosensor. IEEE Sensors Journal, vol. 25, no. 15, 27808-27814, 2025,| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 10. | | FALEIRO, A. S. G.; LINHARES, N. W.; DIAS, D. A. S.; NASCIMENTO, M. T. do; SOUZA, A. L. de; MARTINS. E. de S.; ANDRADE, L. R. M. de. Comparacao de metodologias para determinacao de calcio e magnesio nas rochas. In: REUNIAO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRICAO DE PLANTAS, 26.; REUNIAO BRASILEIRA SOBRE MICORRIZAS, 10.; SIMPOSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 8.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 5., 2004, Lages, SC. FertBio 2004: avaliacao das conquistas: bases para estrategias futuras. Lages: [s. n.], 2004. nao paginado.| Biblioteca(s): Embrapa Cerrados. |
| |
| Registros recuperados : 10 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
