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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
10/12/2021 |
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Data da última atualização: |
10/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS, T. G. S.; JUSTEN, F.; CARNEIRO, C. V. G. C.; HONORATO, V. M.; FRANCO, P. F.; VIEIRA, F. S.; TRICHEZ, D.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de. |
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Afiliação: |
TALITA G.S. RAMOS, Universidade de Brasília; FERNANDA JUSTEN, Universidade de Brasília; CLARA V. G. C. CARNEIRO, Universidade de Brasília; VICTOR M. HONORATO, Universidade de Brasília; PAULA FERNANDES FRANCO, CNPAE; FLÁVIA S. VIEIRA; DÉBORA TRICHEZ; CLENILSON MARTINS RODRIGUES, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Xylonic acid production by recombinant Komagataella phaffii strains engineered with newly identified xylose dehydrogenases. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Bioresource Technology Reports, v. 16, 100825, Dec. 2021. |
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Páginas: |
6 p. |
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Descrição Física: |
PDF: il. |
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ISSN: |
2589-014X |
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DOI: |
https://doi.org/10.1016/j.biteb.2021.100825 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Microbial production of xylonic acid can be achieved via xylose oxidation catalyzed by the xylose dehydrogenase enzyme. In the present study, 11 putative xylose dehydrogenases (XDH) from bacterial and fungi were identified through in silico analysis using the sequences of the two XDH previously described. Among them, 6 genes were successfully cloned and expressed in Komagataella phaffii (Pichia pastoris) as demonstrated by the ability of the strains to produce xylonic acid. The best strain was capable of producing up to 37.1 ± 1.9 and 11.7 ± 1.6 g/L of xylonic acid with yields of 0.96 ± 0.02 and 0.40 ± 0.06 g/g in mineral medium and sugarcane bagasse hydrolysate, respectively. The results presented here demonstrated the functionality of 6 newly identified xylose dehydrogenases, increasing the number of XDH described. In addition, the efficiency of K. phaffii as a xylonic acid producer is showed for the first time. |
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Palavras-Chave: |
Biomass hydrolysate; Komagataella phaffii; Xylonic acid; Xylose dehydrogenase. |
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Thesaurus Nal: |
Biomass; Hydrolysates. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01939naa a2200325 a 4500
001 2137475
005 2021-12-10
008 2021 bl uuuu u00u1 u #d
022 $a2589-014X
024 7 $ahttps://doi.org/10.1016/j.biteb.2021.100825$2DOI
100 1 $aRAMOS, T. G. S.
245 $aXylonic acid production by recombinant Komagataella phaffii strains engineered with newly identified xylose dehydrogenases.$h[electronic resource]
260 $c2021
300 $a6 p.$cPDF: il.
520 $aAbstract: Microbial production of xylonic acid can be achieved via xylose oxidation catalyzed by the xylose dehydrogenase enzyme. In the present study, 11 putative xylose dehydrogenases (XDH) from bacterial and fungi were identified through in silico analysis using the sequences of the two XDH previously described. Among them, 6 genes were successfully cloned and expressed in Komagataella phaffii (Pichia pastoris) as demonstrated by the ability of the strains to produce xylonic acid. The best strain was capable of producing up to 37.1 ± 1.9 and 11.7 ± 1.6 g/L of xylonic acid with yields of 0.96 ± 0.02 and 0.40 ± 0.06 g/g in mineral medium and sugarcane bagasse hydrolysate, respectively. The results presented here demonstrated the functionality of 6 newly identified xylose dehydrogenases, increasing the number of XDH described. In addition, the efficiency of K. phaffii as a xylonic acid producer is showed for the first time.
650 $aBiomass
650 $aHydrolysates
653 $aBiomass hydrolysate
653 $aKomagataella phaffii
653 $aXylonic acid
653 $aXylose dehydrogenase
700 1 $aJUSTEN, F.
700 1 $aCARNEIRO, C. V. G. C.
700 1 $aHONORATO, V. M.
700 1 $aFRANCO, P. F.
700 1 $aVIEIRA, F. S.
700 1 $aTRICHEZ, D.
700 1 $aRODRIGUES, C. M.
700 1 $aALMEIDA, J. R. M. de
773 $tBioresource Technology Reports$gv. 16, 100825, Dec. 2021.
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