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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
05/11/2020 |
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Data da última atualização: |
05/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FACIOLI, F. L.; DE MARCHI, F.; MARQUES, M. G.; MICHELON, P. R. P.; ZANELLA, E. L.; CAIRES, K. C.; REEVES, J. J.; ZANELLA, R. |
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Afiliação: |
FERNANDA L. FACIOLI, UPF; FLÁVIA DE MARCHI, UPF; MARIANA GROKE MARQUES, CNPSA; PAULO R. P. MICHELON, Médico Veterinário; ERALDO LOURENÇO ZANELLA, UPF; KYLE C. CAIRES, University of Hawaii; JERRY J. REEVES, Bar-R Cattle Company; RICARDO ZANELLA, UPF. |
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Título: |
The outcome and economic viability of embryo production using IVF and SOV techniques in the wagyu breed of cattle. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Sciences, v. 7, n. 58, 2020. |
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DOI: |
https://doi.org/10.3390/vetsci7020058 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The Japanese black cattle breed (Wagyu) has an improved metabolism, which allows them to have a higher marbling score when compared with other cattle breeds. However, this may affect other aspects of the animal?s physiology, including hormone secretion and their reproductive success, such as their response to synchronization protocols and embryo production. Therefore, the objectives of this study were to test a superovulation protocol (SOV) developed with low doses of FSH and to evaluate the outcome and economic viability of embryo production using the SOV and in vitro fertilization (IVF) approaches in the Wagyu cattle breed. For that, ten Wagyu cows were submitted to five SOVs over a period of 15 months using a standard protocol: CIDR + 3 mg estradiol benzoate (D0), 35 mg FSH (Folltropin®) a.m. and p.m. (D4), 35 mg Folltropin® a.m. and 20 mg p.m. (D5), 20 mg Folltropin® a.m. and 10 mg p.m. (D6), 10 mg Folltropin® and 0.5 mg cloprostenol, both a.m. and p.m., + CIDR removal (D7), 0.05 mg GnRH + insemination 12 and 24 h after (D8) and embryo collection + 0.5 mg of cloprostenol (D16). Thirty days after each SOV, a follicular aspiration was conducted to produce IVF embryos without any pre-synchronization using standard semen in the same group of animals. The average number of embryos produced was 7.63 +- 5.61 (SOV) and 4.52+- 2.44 (IVF) (p = 0.303). There was no significant correlation between the number of embryos produced by the different techniques (SOV and IVF), indicating that cows that respond well to SOV did not respond well to IVF and vice versa (r = 0.379, p = 0.529). The total cost of each embryo produced by SOV was R$215.00 and R$410.00 for IVF. Therefore, cows that produce less than five embryos by SOV are not economically viable due their lack of response to FSH, and the use of IVF in those animals may be more effective. MenosAbstract: The Japanese black cattle breed (Wagyu) has an improved metabolism, which allows them to have a higher marbling score when compared with other cattle breeds. However, this may affect other aspects of the animal?s physiology, including hormone secretion and their reproductive success, such as their response to synchronization protocols and embryo production. Therefore, the objectives of this study were to test a superovulation protocol (SOV) developed with low doses of FSH and to evaluate the outcome and economic viability of embryo production using the SOV and in vitro fertilization (IVF) approaches in the Wagyu cattle breed. For that, ten Wagyu cows were submitted to five SOVs over a period of 15 months using a standard protocol: CIDR + 3 mg estradiol benzoate (D0), 35 mg FSH (Folltropin®) a.m. and p.m. (D4), 35 mg Folltropin® a.m. and 20 mg p.m. (D5), 20 mg Folltropin® a.m. and 10 mg p.m. (D6), 10 mg Folltropin® and 0.5 mg cloprostenol, both a.m. and p.m., + CIDR removal (D7), 0.05 mg GnRH + insemination 12 and 24 h after (D8) and embryo collection + 0.5 mg of cloprostenol (D16). Thirty days after each SOV, a follicular aspiration was conducted to produce IVF embryos without any pre-synchronization using standard semen in the same group of animals. The average number of embryos produced was 7.63 +- 5.61 (SOV) and 4.52+- 2.44 (IVF) (p = 0.303). There was no significant correlation between the number of embryos produced by the different techniques (SOV and IVF), in... Mostrar Tudo |
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Palavras-Chave: |
Fertilização in vitro; Gado Preto Japonês; Produção de embriões; Raça wagyu de gado. |
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Thesagro: |
Genética Animal; Reprodução Animal; Superovulação. |
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Thesaurus Nal: |
Animal reproduction; In vitro fertilization; Superovulation; Wagyu. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02931naa a2200349 a 4500
001 2126316
005 2020-11-05
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/vetsci7020058$2DOI
100 1 $aFACIOLI, F. L.
245 $aThe outcome and economic viability of embryo production using IVF and SOV techniques in the wagyu breed of cattle.$h[electronic resource]
260 $c2020
520 $aAbstract: The Japanese black cattle breed (Wagyu) has an improved metabolism, which allows them to have a higher marbling score when compared with other cattle breeds. However, this may affect other aspects of the animal?s physiology, including hormone secretion and their reproductive success, such as their response to synchronization protocols and embryo production. Therefore, the objectives of this study were to test a superovulation protocol (SOV) developed with low doses of FSH and to evaluate the outcome and economic viability of embryo production using the SOV and in vitro fertilization (IVF) approaches in the Wagyu cattle breed. For that, ten Wagyu cows were submitted to five SOVs over a period of 15 months using a standard protocol: CIDR + 3 mg estradiol benzoate (D0), 35 mg FSH (Folltropin®) a.m. and p.m. (D4), 35 mg Folltropin® a.m. and 20 mg p.m. (D5), 20 mg Folltropin® a.m. and 10 mg p.m. (D6), 10 mg Folltropin® and 0.5 mg cloprostenol, both a.m. and p.m., + CIDR removal (D7), 0.05 mg GnRH + insemination 12 and 24 h after (D8) and embryo collection + 0.5 mg of cloprostenol (D16). Thirty days after each SOV, a follicular aspiration was conducted to produce IVF embryos without any pre-synchronization using standard semen in the same group of animals. The average number of embryos produced was 7.63 +- 5.61 (SOV) and 4.52+- 2.44 (IVF) (p = 0.303). There was no significant correlation between the number of embryos produced by the different techniques (SOV and IVF), indicating that cows that respond well to SOV did not respond well to IVF and vice versa (r = 0.379, p = 0.529). The total cost of each embryo produced by SOV was R$215.00 and R$410.00 for IVF. Therefore, cows that produce less than five embryos by SOV are not economically viable due their lack of response to FSH, and the use of IVF in those animals may be more effective.
650 $aAnimal reproduction
650 $aIn vitro fertilization
650 $aSuperovulation
650 $aWagyu
650 $aGenética Animal
650 $aReprodução Animal
650 $aSuperovulação
653 $aFertilização in vitro
653 $aGado Preto Japonês
653 $aProdução de embriões
653 $aRaça wagyu de gado
700 1 $aDE MARCHI, F.
700 1 $aMARQUES, M. G.
700 1 $aMICHELON, P. R. P.
700 1 $aZANELLA, E. L.
700 1 $aCAIRES, K. C.
700 1 $aREEVES, J. J.
700 1 $aZANELLA, R.
773 $tVeterinary Sciences$gv. 7, n. 58, 2020.
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Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 7 | |
| 1. |  | TAKANO, P. V.; BLAGITZ, M. G.; MIRA, C. S.; BATISTA, C. F.; DELLA LIBERA, A. M. M.; SOUZA, F. N. Estudo comparativo das diferentes técnicas empregadas na contagem diferencial de leucócitos no leite. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 773-778, abril 2018 Título em inglês: Comparative study of distinct techniques to determine differential leukocyte counts in milk. .| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | MOLINARI, P. C. C.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; BATISTA, C. F.; SOUZA, F. N. Intracellular reactive oxygen species production and phagocytosis of Staphylococcus aureus by milk neutrophils as tool to diagnose mastitis and identify susceptible dairy cows. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 659-664, abril 2018 Título em português: Produção intracelular de espécies reativas de oxigênio e fagocitose de Staphylococcus aureus por neutrófilos lácteos como ferramentas para o diagnóstico da mastite e identificação de vacas leiteiras susceptíveis à...| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | BLAGITZ, M. G.; SOUZA, F. N.; BATISTA, C. F.; SANTOS, BRUNA P.; PARRA, A. C.; AZEVEDO, L. F. F.; DELLA LIBERA, A. M. M. P. Expression of CD14 and toll-like receptors 2 and 4 by milk neutrophils in bovine mammary glands infected with Corynebacterium bovis. Pesquisa Veterinária Brasileira, Rio de Janeiro, n. 35, n.1, p.1-5, jan. 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 4. | | VIANNA, R. S.; BATISTA, C. F.; LIMA, D. M.; SANTOS, K. R.; GOMES, R. C.; BERTAGNON, H. G.; DELLA LIBERA, A. M. M. P. Perfil citológico pulmonar de bezerros sadios nos três primeiros meses de vida. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 447-452, maio. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | BERTAGNON, H. G.; BATISTA, C. F.; BELLINAZZI, J. B.; CONEGLIAN, M. M.; MENDES, A. F.; DELLA LIBERA, A. M. M. P. Identificação de dor após orquiectomia em garrotes: desenvolvimento de escala análogo visual em comparação a parâmetros fisiológicos, padrões comportamentais e expressão facial. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 3, p. 436-443, março 2018 Título em inglês: Pain identification after orchiectomy in young bulls: development of the visual analogue scale compared with physiological parameters, behavioral patterns and facial expression.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 6. | | MARTINS, E. R. M.; BERTAGNON, H. G.; BATISTA, C. F.; GOMES, R. C.; SANTOS, K. R.; BELLINAZZI, J. B.; DELLA LIBERA, A. M. M. P. Influência da suplementação de vitaminas A, D e E na função imune de bezerros alimentados com dieta à base de feno capim tifton (Cynodon ssp.). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 453-459, maio. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 7. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N.; LAGE, A. P.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 811-818, set. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 7 | |
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