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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
10/05/2023 |
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Autoria: |
HODGSON, A. L. M.; BIRD, P.; NISBET, I. T. |
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Título: |
Cloning, nucleotide sequence, and expression in Escherichia coli of the phospholipase D gene from Corynebacterium pseudotuberculosis. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Journal of Bacteriology, v. 172, n. 3, p. 1256-1261, 1990. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The phospholipase D (PLD) gene from Corynebacterium pseudotuberculosis has been cloned, sequenced, and expressed in Escherichia coli. Analysis of DNA sequence data reveals a major open reading frame encoding a 31.4-kilodalton protein, a size consistent with that estimated for the PLD protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Comparison of these data with the amino-terminal protein sequence indicates that the mature PLD protein is preceded by a 24-residue signal sequence. Expression of the PLD gene in E. coli is initiated from the corynebacterial promoter, and the resulting protein has sphingomyelinase activity. Primer extension mapping localized the 5' end of the PLD gene mRNA to a site 5 to 7 base pairs downstream of a region similar to the consensus sequence for E. coli promoters. Northern and Southern blot analyses suggest that the gene is transcribed from mRNA approximately 1.1 kilobases in length and that it is present in a single copy within the C. pseudotuberculosis genome. |
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Palavras-Chave: |
Bacterial diseases. |
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Thesagro: |
Bactéria; Corynebacterium Pseudotuberculosis; Doença; Linfadenite Caseosa; Ovino. |
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Thesaurus Nal: |
Bacterial infections; Caseous lymphadenitis; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01830naa a2200253 a 4500
001 1521765
005 2023-05-10
008 1990 bl uuuu u00u1 u #d
100 1 $aHODGSON, A. L. M.
245 $aCloning, nucleotide sequence, and expression in Escherichia coli of the phospholipase D gene from Corynebacterium pseudotuberculosis.$h[electronic resource]
260 $c1990
520 $aAbstract: The phospholipase D (PLD) gene from Corynebacterium pseudotuberculosis has been cloned, sequenced, and expressed in Escherichia coli. Analysis of DNA sequence data reveals a major open reading frame encoding a 31.4-kilodalton protein, a size consistent with that estimated for the PLD protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Comparison of these data with the amino-terminal protein sequence indicates that the mature PLD protein is preceded by a 24-residue signal sequence. Expression of the PLD gene in E. coli is initiated from the corynebacterial promoter, and the resulting protein has sphingomyelinase activity. Primer extension mapping localized the 5' end of the PLD gene mRNA to a site 5 to 7 base pairs downstream of a region similar to the consensus sequence for E. coli promoters. Northern and Southern blot analyses suggest that the gene is transcribed from mRNA approximately 1.1 kilobases in length and that it is present in a single copy within the C. pseudotuberculosis genome.
650 $aBacterial infections
650 $aCaseous lymphadenitis
650 $aSheep diseases
650 $aBactéria
650 $aCorynebacterium Pseudotuberculosis
650 $aDoença
650 $aLinfadenite Caseosa
650 $aOvino
653 $aBacterial diseases
700 1 $aBIRD, P.
700 1 $aNISBET, I. T.
773 $tJournal of Bacteriology$gv. 172, n. 3, p. 1256-1261, 1990.
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| 1. |  | CAVALIERI, S. D.; SILVA, F. M. L.; VELINI, E. D.; SÃO JOSÉ, A. R.; ULLOA, S. M.; DATTA, A.; CAVALIERI, J. D.; KNEZEVIC, S. Z. Seletividade do nicosulfuron em três estádios fenológicos de milho-pipoca. Planta Daninha, Viçosa, MG, v. 30, n. 2, p. 377-386, abr./jun. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 2. | | BUTLER, E. E.; DATTA, A.; FLORES-MORENO, H.; CHEN, M.; WYTHERS, K. R.; FAZAYELI, F.; BANERJEE, A.; ATKIN, O. K.; KATTGE, J.; AMIAUD, B.; BLONDER, B.; BOENISCH, G.; BOND-LAMBERTY, B.; BROWN, K. A.; BYUN, C.; CAMPETELLA, G.; CERABOLINI, B. E. L.; CORNELISSEN, J. H. C.; CRAINE, J. M.; CRAVEN, D.; DE VRIES, F. T.; DIAZ, S.; DOMINGUES, T. F.; FOREY, E.; GONZALEZ-MELO, A.; GROSS, N.; HAN, W.; HATTINGH, W. N.; HICKLER, T.; JANSEN, S.; SOSINSKI JUNIOR, E. E.; KRAMER, K. Mapping local and global variability in plant trait distributions. PNAS, v. 114, n. 51, Dec. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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