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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
11/12/2020 |
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Data da última atualização: |
11/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARANTES, L. G.; TONELLI, G. S. S. S.; MARTINS, C. F.; BÁO, S. N. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC. |
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Título: |
Cellular Characterization and Effects Of Cryoprotectant Solutions on the Viability Of Fibroblasts from Three Brazilian Wild Cats. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Biopreservation and Biobanking, 2020. |
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Idioma: |
Português |
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Conteúdo: |
Preserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna. MenosPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating... Mostrar Tudo |
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Palavras-Chave: |
Material genético; Protocolo. |
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Thesagro: |
Criopreservação. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02696naa a2200193 a 4500
001 2128006
005 2020-12-11
008 2020 bl uuuu u00u1 u #d
100 1 $aARANTES, L. G.
245 $aCellular Characterization and Effects Of Cryoprotectant Solutions on the Viability Of Fibroblasts from Three Brazilian Wild Cats.$h[electronic resource]
260 $c2020
520 $aPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna.
650 $aCriopreservação
653 $aMaterial genético
653 $aProtocolo
700 1 $aTONELLI, G. S. S. S.
700 1 $aMARTINS, C. F.
700 1 $aBÁO, S. N.
773 $tBiopreservation and Biobanking, 2020.
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Embrapa Cerrados (CPAC) |
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| Registros recuperados : 11 | |
| 4. |  | SILVA, R. A. M. S.; VICTORIO, A. M.; RAMIREZ, L.; D'AVILA, A. M. R.; TRAJANO, V.; JANSEN, A. M. Hematological and blood chemistry alterations in coatis (Nasua nasua) naturally infected by Trypanosoma evansi in the Pantanal, Brazil. Revue de l'Élevage et Médicine Véterinaire des Pays Tropicaux, v.52, n.2, p.119-122, 1999.| Biblioteca(s): Embrapa Pantanal; Embrapa Suínos e Aves. |
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| 5. | | D'ÁVILA, A.; KICH, J. D.; ESTEVES, P. A.; BIESUS, L. L.; PISSETTI, C.; COLDEBELLA, A.; TRIQUES, N. J.; KLEIN, C. S. Padronização e validação de uma técnica de PCR com controle interno para detecção de Salmonella sp. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 14., 2009, Uberlândia. Anais... Uberlândia: Abraves, 2009. p. 357-358. 1 CD-ROM. Subprojeto: 02.06.60.400-02| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 6. | | QUEIROZ, A. O.; CORREA, R. P. G.; MENEZES, V. T.; D'AVILA, A. M. R.; SILVA, R. A. M. S.; LEON, L. L.; JANSEN, A. M. Isoenzymatic characterization of isolates of T. evansi from the Pantanal in Mato Grosso State, Brazil. Memorias do Instituto Oswaldo Cruz, v.92, p.130, Nov. 1997. Supplement 1.| Biblioteca(s): Embrapa Pantanal. |
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| 7. | | ROOS, D.; KLEIN, T. A. P.; ESTEVES, P. A.; D'ÁVILA, A.; MUNHOZ, L. S.; BRENTANO, L.; OKINO, C. H.; TREVISOL, I. M.; CARON, L.; SILVA, V. S. Identificação do vírus de bronquite infecciosa das galinhas através de isolamento em ovos SPF e técnicas de diagnóstico molecular. In: JORNADA DE INICIAÇÃO CIENTÍFICA EMBRAPA , 5., 2011, Concórdia. Resumos. Concórdia: Embrapa Suínos e Aves, 2011. p. 47 . JINC. Projeto/Plano de Ação: 02.09.01.030.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | KLEIN, T. E.; COSTA, C.; RITTERBUSCH, G. A.; D'ÁVILA, A.; OKINO, C. H.; TREVISOL, I. M.; ESTEVES, P. A.; BRENTANO, L. Detecção do vírus da bronquite infecciosa das galinhas a partir de líquido e membrana corioalantóide. In: JORNADA DE INICIAÇÃO CIENTÍFICA, 6.; SEMINÁRIO INTEGRADO DE PESQUISA E EXTENSÃO DA UnC, 2., 2012, Concórdia. Anais... Concórdia: Embrapa Suínos e Aves, 2012. p. 22. JINC. SIPEX. Projeto/Plano de Ação: 02.09.13.000-01.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 9. | | SILVA, V. S.; MORES, N.; AMARAL, A. L. do; KRAMER, B.; ESTEVES, P. A.; D’ÁVILA, A.; SCHAEFER, R.; DUTRA, V.; FERREIRA NETO, J. S.; FERREIRA, F.; VENTURA, L. das V.; COLDEBELLA, A. Epidemiology and control of swine mycobacteriosis In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1, Campo Grande, MS, 2009. Anais.... Campo Grande: Embrapa Gado de Corte, Labex-USA, CNPq, 2009. 1 CD-ROM. Projeto/Plano de Ação: 16.00.30.004-00| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 10. | | SILVA, V. S.; AMARAL, A. L. do; KRAMER, B.; D'AVILA, A.; ESTEVES, P. A.; SCHAEFER, R.; HIGARASHI, M. M.; PAIVA, D. P. de; MATTEI, R. M.; KUNZ, A.; OLIVEIRA, P. A. V. de; MINOSO, M.; COLDEBELLA, A. Estudo da sobrevivência de MAC em camas de maravalha e casca de arroz experimentalmente contaminadas, ao longo da passagem de quatro lotes consecutivos de suínos. In: COLDEBELLA, A.; SCHEUERMANN, G.N. (Ed.). Relatório dos projetos concluídos 2010. Concórdia: Embrapa Suínos e Aves, 2011. (Embrapa Suínos e Aves. Documentos, 144). p. 93-99. Projeto: 03.04.03.051.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 11. | | LEON, L. L; CANTO-CAVALHEIRO, M.M.; TRAJANO-MENEZES, V.; SILVA, R. A. M. S.; D'AVILA, A. M. R.; ECHEVARRIA, A.; SANTOS, L. H. S.; GOMES CARDOSO, L.; JANSEN, A. M. Synthetic amidines: a possible new therapeutic choice for the treatment of "Mal de cadeiras". Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.91, p.107, 1996. Suplemento.| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 11 | |
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