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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
30/07/2024 |
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Data da última atualização: |
13/12/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIANA, J. H. M.; MOURA, R. M. da; MARTINS, L. P.; FIGUEIREDO, R. A.; SIQUEIRA, L. G. B.; FERNANDES, C. A. C. |
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Afiliação: |
JOAO HENRIQUE MOREIRA VIANA, CENARGEN; RODRIGO MARTINS DE MOURA, UNIVERSIDADE DE BRASÍLIA; LETICIA PRATA MARTINS, UNIVERSIDADE DE BRASÍLIA; RICARDO ALAMINO FIGUEIREDO, CENARGEN; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; CARLOS ANTONIO CARVALHO FERNANDES, UNIVERSIDADE PROF. EDSON ANTONIO VELANO. |
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Título: |
Superovulating cattle with corifollitropin-alpha, a long-acting recombinant human FSH (rhFSH): dose-response, half-life, effects on the ovaries, and embryo outcomes. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Theriogenology, v. 226, p. 302-307, 2024. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2024.06.033 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the superestimulatory and superovulatory responses of cattle treated with corifollitropin-alpha, a long-acting human recombinant FSH (rhFSH). In the first and second experiments, we used Nelore (Bos indicus) heifers previously submitted to follicular wave suppression by active immunization against GnRH. In Experiment 1 (a dose-response study), heifers (n = 20) were randomly allocated into five groups, which received placebo (saline) or a single sc dose of 7.5, 15.0, 22.5 or 30.0 μg rhFSH. The heifers were subjected to daily ovarian scan and blood sampling during 11 days. We observed group, time, and group x time effects (P<0.0001) for both average follicle size and circulating FSH concentrations, with a strong correlation (R = 0.82, P<0.0001) between the area under curve (AUC) for both parameters. The peak concentration of FSH 24h after treatment and average follicle size at all timepoints, however, were similar (P>0.05) between groups 22.5 and 30.0 μg. In Experiment 2, heifers (n = 18) were allocated into three groups, which received (0h) either placebo (control), 25 μg rhFSH or 130 mg pFSH (Folltropin). There was no difference (P>0.05) in average follicle size at any moment, as well as in intrafollicular E2 at 120h or in plasma P4 seven days later between groups rhFSH and pFSH. In Experiment 3, cycling Nelore heifers (n = 20) were subjected to a wave synchronization protocol and superovulated (day 0) using a standard pFSH protocol (120 mg split in eight decreasing im doses) or with a single sc injection of 20 μg rhFSH. The number of follicles >7 mm on day 4 did not differ (P=0.4370). Heifers receiving rhFSH had greater average follicle size on day 4 (P=0.0005), ovulation rate (P<0.0001), and number of CL (P=0.0155), as well as a trend towards a greater number of ova (P=0.07) and viable embryos (P=0.0590). In Experiment 4, superovulation was induced with a single sc injection of 25 μg rhFSH in Girolando and Nelore cows and heifers (n = 20). None of the embryo yield endpoints differed between the two breeds (P>0.05). In conclusion, cattle superstimulation and superovulation can be successfully induced with a single dose of a long-acting rhFSH (corifollitropin-alpha). MenosThe aim of this study was to evaluate the superestimulatory and superovulatory responses of cattle treated with corifollitropin-alpha, a long-acting human recombinant FSH (rhFSH). In the first and second experiments, we used Nelore (Bos indicus) heifers previously submitted to follicular wave suppression by active immunization against GnRH. In Experiment 1 (a dose-response study), heifers (n = 20) were randomly allocated into five groups, which received placebo (saline) or a single sc dose of 7.5, 15.0, 22.5 or 30.0 μg rhFSH. The heifers were subjected to daily ovarian scan and blood sampling during 11 days. We observed group, time, and group x time effects (P<0.0001) for both average follicle size and circulating FSH concentrations, with a strong correlation (R = 0.82, P<0.0001) between the area under curve (AUC) for both parameters. The peak concentration of FSH 24h after treatment and average follicle size at all timepoints, however, were similar (P>0.05) between groups 22.5 and 30.0 μg. In Experiment 2, heifers (n = 18) were allocated into three groups, which received (0h) either placebo (control), 25 μg rhFSH or 130 mg pFSH (Folltropin). There was no difference (P>0.05) in average follicle size at any moment, as well as in intrafollicular E2 at 120h or in plasma P4 seven days later between groups rhFSH and pFSH. In Experiment 3, cycling Nelore heifers (n = 20) were subjected to a wave synchronization protocol and superovulated (day 0) using a standard pFSH protocol (120... Mostrar Tudo |
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Palavras-Chave: |
Superestimulação; Superstimulation; Transferência de embriões. |
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Thesagro: |
Bovino; Gado Zebu; Gonadotrofina; Reprodução Animal. |
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Thesaurus Nal: |
Embryo transfer; Gonadotropins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03248naa a2200301 a 4500
001 2166075
005 2024-12-13
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2024.06.033$2DOI
100 1 $aVIANA, J. H. M.
245 $aSuperovulating cattle with corifollitropin-alpha, a long-acting recombinant human FSH (rhFSH)$bdose-response, half-life, effects on the ovaries, and embryo outcomes.$h[electronic resource]
260 $c2024
520 $aThe aim of this study was to evaluate the superestimulatory and superovulatory responses of cattle treated with corifollitropin-alpha, a long-acting human recombinant FSH (rhFSH). In the first and second experiments, we used Nelore (Bos indicus) heifers previously submitted to follicular wave suppression by active immunization against GnRH. In Experiment 1 (a dose-response study), heifers (n = 20) were randomly allocated into five groups, which received placebo (saline) or a single sc dose of 7.5, 15.0, 22.5 or 30.0 μg rhFSH. The heifers were subjected to daily ovarian scan and blood sampling during 11 days. We observed group, time, and group x time effects (P<0.0001) for both average follicle size and circulating FSH concentrations, with a strong correlation (R = 0.82, P<0.0001) between the area under curve (AUC) for both parameters. The peak concentration of FSH 24h after treatment and average follicle size at all timepoints, however, were similar (P>0.05) between groups 22.5 and 30.0 μg. In Experiment 2, heifers (n = 18) were allocated into three groups, which received (0h) either placebo (control), 25 μg rhFSH or 130 mg pFSH (Folltropin). There was no difference (P>0.05) in average follicle size at any moment, as well as in intrafollicular E2 at 120h or in plasma P4 seven days later between groups rhFSH and pFSH. In Experiment 3, cycling Nelore heifers (n = 20) were subjected to a wave synchronization protocol and superovulated (day 0) using a standard pFSH protocol (120 mg split in eight decreasing im doses) or with a single sc injection of 20 μg rhFSH. The number of follicles >7 mm on day 4 did not differ (P=0.4370). Heifers receiving rhFSH had greater average follicle size on day 4 (P=0.0005), ovulation rate (P<0.0001), and number of CL (P=0.0155), as well as a trend towards a greater number of ova (P=0.07) and viable embryos (P=0.0590). In Experiment 4, superovulation was induced with a single sc injection of 25 μg rhFSH in Girolando and Nelore cows and heifers (n = 20). None of the embryo yield endpoints differed between the two breeds (P>0.05). In conclusion, cattle superstimulation and superovulation can be successfully induced with a single dose of a long-acting rhFSH (corifollitropin-alpha).
650 $aEmbryo transfer
650 $aGonadotropins
650 $aBovino
650 $aGado Zebu
650 $aGonadotrofina
650 $aReprodução Animal
653 $aSuperestimulação
653 $aSuperstimulation
653 $aTransferência de embriões
700 1 $aMOURA, R. M. da
700 1 $aMARTINS, L. P.
700 1 $aFIGUEIREDO, R. A.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aFERNANDES, C. A. C.
773 $tTheriogenology$gv. 226, p. 302-307, 2024.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| 3. |  | BACKES, R. L.; VOGT, G. A.; ZOLDAN, S. R.; DÁVALOS, E. D.; HOFS, A.; STENGER, E. A. F.; EMYGDIO, B. M. Avaliação preliminar de variedades de polinização aberta de milho em Santa Catarina nas safras 2009/10 e 2010/11. In: REUNIÃO TÉCNICA CATARINENSE DE MILHO E FEIJÃO, 8., 2011, Chapecó. Anais. Resumos... Chapecó: EPAGRI, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 4. | | BACKES, R. L.; VOGT, G. A.; BALBINOT JUNIOR, A. A.; DÁVALOS, E. D.; HÖFS, A.; ZOLDAN, S. R.; EMYGDIO, B. M. Ensaio regional de variedades de polinização aberta de milho em Santa Catarina na safra 2010/11. In: REUNIÃO TÉCNICA CATARINENSE DE MILHO E FEIJÃO, 8., 2011, Chapecó. Resumos expandidos... Chapecó: Epagri, 2011. 5 p. Seção Milho, 1.11. CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Soja. |
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| 5. | | DEL DUCA, L. de J. A.; SOUSA, C. N. A. de; LINHARES, A. G.; NASCIMENTO JUNIOR, A. do; BARCELLOS, A. L.; PRESTES, A. M.; ZANATTA, A. C. A.; IORCZESKI, E. J.; GUARIENTI, E. M.; BEVILAQUA, G. A. P.; CUNHA, G. R. da; COSTAMILAN, L. M.; SÓ e SILVA, M.; LIMA, M. I. P. M.; MIRANDA, M. Z. de; FONTANELI, R. S.; RODRIGUES, O.; SCHEEREN, P. L.; BRAMMER, S. P.; CAETANO, V. R.; ZANOTELLI, V.; DÁVALOS, E. D.; VIEIRA, L. C. Desenvolvimento de genótipos de trigo para a região tritícola sul-brasileira. Passo Fundo: Embrapa Trigo, 2002. 23 p. html. Publicação Online. (Embrapa Trigo. Boletim de Pesquisa e Desenvolvimento Online, 10).| Biblioteca(s): Embrapa Trigo. |
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