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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Pecuária Sul. |
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Data corrente: |
10/02/2020 |
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Data da última atualização: |
01/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALVES, J. R. A.; FARIAS, A. E. M. de; ANJOS, D. M. dos; LIMA, A. M. C.; FACCIOLI-MARTINS, P. Y.; SOUZA, C. J. H. de; PINHEIRO, R. R.; ALVES, F. S. F.; AZEVEDO, S. S. de; ALVES, C. J. |
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Afiliação: |
JOSÉ ROMERO ALEXANDRE ALVES; AREANO ETHÉRIO MOREIRA DE FARIAS; DENIZE MONTEIRO DOS ANJOS; ANA MILENA CÉZAR LIMA; PATRICIA YOSHIDA FACCIOLI-MARTINS, CNPC; CARLOS JOSÉ HOFF DE SOUZA; RAYMUNDO RIZALDO PINHEIRO, CNPC; FRANCISCO SELMO FERNANDES ALVES, CNPC; SÉRGIO SANTOS DE AZEVEDO; CLEBERT JOSÉ ALVES. |
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Título: |
Seroepidemiological study of Caseous lymphadenitis in sheep from the Northeast region of Brazil using an indirect ELISA. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Tropical Animal Health and Production, v. 52, n. 4, p. 1945-1952, Jul. 2020. |
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DOI: |
10.1007/s11250-020-02214-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The objective of this study was to determine the frequency of seropositivity Corynebacterium pseudotuberculosis in sheep in five states of northeastern Brazil, using an indirect enzyme-linked immunosorbent assay (i-ELISA). Young and adult sheep of both sexes were used. Blood samples were collected from 2638 sheep from 223 herds across all states. For the i-ELISA, antigens produced from the strain of C. pseudotuberculosis BRM 029971, a bacterial isolate from the Northeast region of Brazil, were used. Sensitivity and specificity indexes were calculated for the validation of the test, using as reference 49 and 134 serum samples from sheep known to be positive and negative, respectively. The i-ELISA presented four false-negative and four false-positive results, showing a specificity of 97.01%, a sensitivity of 91.84%, and an accuracy of 95.63%. These results were calculated based on an optical density (OD) cutoff point?=?0.138. Of the 2638 sheep tested, 996 (37.76%, 95% CI?=?35.93-39.62%) were seropositive, and of the 223 evaluated herds, 210 (94.17%, 95% CI?=?90.28-96.56%) seropositive. The i-ELISA showed adequate sensitivity and specificity, proving to be a useful tool in the diagnosis of caseous lymphadenitis (CLA) in sheep. Infection by C. pseudotuberculosis, determined by serology, is disseminated in the sheep herds in the states of Northeast Brazil. Thus, there is a need to implement effective control measures that prevent the spread of infectious agents. |
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Palavras-Chave: |
Brazilian northeast; Sorologia. |
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Thesagro: |
Bactéria; Bacteriose; Corynebacterium Pseudotuberculosis; Doença Animal; Elisa; Linfadenite Caseosa; Ovino. |
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Thesaurus Nal: |
Caseous lymphadenitis; Enzyme-linked immunosorbent assay; Epidemiological studies; Serology; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02747naa a2200409 a 4500
001 2120073
005 2020-12-01
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s11250-020-02214-9$2DOI
100 1 $aALVES, J. R. A.
245 $aSeroepidemiological study of Caseous lymphadenitis in sheep from the Northeast region of Brazil using an indirect ELISA.$h[electronic resource]
260 $c2020
520 $aAbstract: The objective of this study was to determine the frequency of seropositivity Corynebacterium pseudotuberculosis in sheep in five states of northeastern Brazil, using an indirect enzyme-linked immunosorbent assay (i-ELISA). Young and adult sheep of both sexes were used. Blood samples were collected from 2638 sheep from 223 herds across all states. For the i-ELISA, antigens produced from the strain of C. pseudotuberculosis BRM 029971, a bacterial isolate from the Northeast region of Brazil, were used. Sensitivity and specificity indexes were calculated for the validation of the test, using as reference 49 and 134 serum samples from sheep known to be positive and negative, respectively. The i-ELISA presented four false-negative and four false-positive results, showing a specificity of 97.01%, a sensitivity of 91.84%, and an accuracy of 95.63%. These results were calculated based on an optical density (OD) cutoff point?=?0.138. Of the 2638 sheep tested, 996 (37.76%, 95% CI?=?35.93-39.62%) were seropositive, and of the 223 evaluated herds, 210 (94.17%, 95% CI?=?90.28-96.56%) seropositive. The i-ELISA showed adequate sensitivity and specificity, proving to be a useful tool in the diagnosis of caseous lymphadenitis (CLA) in sheep. Infection by C. pseudotuberculosis, determined by serology, is disseminated in the sheep herds in the states of Northeast Brazil. Thus, there is a need to implement effective control measures that prevent the spread of infectious agents.
650 $aCaseous lymphadenitis
650 $aEnzyme-linked immunosorbent assay
650 $aEpidemiological studies
650 $aSerology
650 $aSheep diseases
650 $aBactéria
650 $aBacteriose
650 $aCorynebacterium Pseudotuberculosis
650 $aDoença Animal
650 $aElisa
650 $aLinfadenite Caseosa
650 $aOvino
653 $aBrazilian northeast
653 $aSorologia
700 1 $aFARIAS, A. E. M. de
700 1 $aANJOS, D. M. dos
700 1 $aLIMA, A. M. C.
700 1 $aFACCIOLI-MARTINS, P. Y.
700 1 $aSOUZA, C. J. H. de
700 1 $aPINHEIRO, R. R.
700 1 $aALVES, F. S. F.
700 1 $aAZEVEDO, S. S. de
700 1 $aALVES, C. J.
773 $tTropical Animal Health and Production$gv. 52, n. 4, p. 1945-1952, Jul. 2020.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 7. |  | CRESTANA, S. Pesquisa, tecnologia e inovação. In: Congresso de Agribusiness, 13., 2012, Rio de Janeiro. Agronegócio e Sustentabilidade: anais. Rio de Janeiro: Sociedade Nacional de Agricultura, 2012. p. 64-76.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 15. | | CRESTANA, S. A Embrapa e a agroenergia. In: VELLOSO, J. P. dos R.; ALBUQUERQUE, R. C. de (Coord.). Chegou a vez do Brasil? Oportunidade para a geração de brasileiros que nunca viu o país crescer. Rio de Janeiro: Editora José Olympio Ltda, 2007. 741 p. Textos apresentados no XIX Fórum Nacional. p. 476-484| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Unidades Centrais. |
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| 18. | | CRESTANA, S. A indústria brasileira de bens de capital mecânicos, o SIAGRO e a necessidade urgente de melhoria do ecossistema institucional brasileiro. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 731-734. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 553 | |
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