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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
13/03/2014 |
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Data da última atualização: |
19/10/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FARIA, M. T. de; CURY-BOAVENTURA, M. F.; LOPES, L. R.; SILVA, J. R. M. C. da. |
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Afiliação: |
MARCOS TUCUNDUVA DE FARIA, CPATU; Maria Fernanda Cury-Boaventura, USP; Lucia Rossetti Lopes, USP; José Roberto Machado Cunha da Silva, USP. |
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Título: |
Generation of reactive oxygen species by leukocytes of Prochilodus lineatus. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Fish Physiology and Biochemistry, v. 40, n. 2, p. 445-455, Apr. 2014. |
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DOI: |
10.1007/s10695-013-9856-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Prochilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species. MenosProchilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the r... Mostrar Tudo |
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Palavras-Chave: |
Citometria de fluxo; Espécies reativas de oxigênio; Explosão respiratória; Mitocôndrias. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02340naa a2200217 a 4500
001 1982284
005 2022-10-19
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s10695-013-9856-9$2DOI
100 1 $aFARIA, M. T. de
245 $aGeneration of reactive oxygen species by leukocytes of Prochilodus lineatus.$h[electronic resource]
260 $c2014
520 $aProchilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species.
653 $aCitometria de fluxo
653 $aEspécies reativas de oxigênio
653 $aExplosão respiratória
653 $aMitocôndrias
700 1 $aCURY-BOAVENTURA, M. F.
700 1 $aLOPES, L. R.
700 1 $aSILVA, J. R. M. C. da
773 $tFish Physiology and Biochemistry$gv. 40, n. 2, p. 445-455, Apr. 2014.
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| 3. |  | JANK, L.; COSTA, R. de A.; CHIARI, L.; RESENDE, R. M. S.; VALLE, C. B. do. Potencial de utilização do banco ativo de germoplasma da forrageira de Panicum maximum JACQ. como fonte de energia. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304). Editora técnica Clara Oliveira Goedert. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | MAYER, L. R. R.; JANK, L.; LEMPP, B.; COSTA, R. de A. Seleção de genótipos de Panicum maximum (Megathyrsus maximus) para produção de bioenergia. In:SIMPÓSIO INTERNACIONAL SOBRE MELHORAMENTO DE FORRAGEIRAS, 2., 2009, Campo Grande, MS. [Anais]... Campo Grande, MS: Embrapa Gado de Corte, 2009. II SIMF. Comitê editorial: Liana Jank; Lucimara Chiari; Rosângela Maria Simeão Resende. 4 p. 1 CD-ROM. Resumo M 37.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 6. | | BRASILIENSE, A. R. P.; MARTINS, A. C. D.; CASTELO, A. S.; SILVA, B. M. P. da; COSTA, R. de A.; YOSHIOKA, E. T. O. Hematologia e bioquímica de tracajá após restrição e realimentação. In: JORNADA CIENTÍFICA DA EMBRAPA AMAPÁ, 1., 2015, Macapá. Resumos... Macapá: Embrapa Amapá, 2015. 1 CD ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amapá. |
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| 7. | | YOSHIOKA, E. T. O.; CASTELO, A. S.; COSTA, R. de A.; SILVA, B. M. P. da; BRASILIENSE, A. R. P.; MARTINS, A. C. D. Crescimento e variáveis hematológicas de tracajás (Podocnemis unifilis) aos seis e dezoito meses de vida. In: ENCONTRO BRASILEIRO DE PATOLOGISTAS DE ORGANISMOS AQUÁTICOS, 13., 2014, Aracaju. Anais.... Aracaju: ABRAPOA, 2014. 1 CD-ROM. ENBRAPOA. HEM011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amapá. |
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| 8. | | YOSHIOKA, E. T. O.; COSTA, R. de A.; BRASILIENSE, A. R. P.; CASTELO, A. S.; MARTINS, A. C. D.; SILVA, B. M. P. da; DAMASCENO, L. F. Perfil hematológico e bioquímico de filhotes de tracajás (Podocnemis unifilis) alimentados com níveis variados de proteína bruta. In: TAVARES-DIAS, M.; MARIANO, W. dos S. (Org.). Aquicultura no Brasil: novas perspectivas. São Carlos: Pedro & João Editores, 2015. v. 1, cap. 2, p. 31-44. v. 1| Tipo: Capítulo em Livro Técnico-Científico |
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