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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
12/05/2021 |
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Data da última atualização: |
22/08/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ANDRADE, J. de S.; MOREIRA, E. M.; SILVA, G. M. da; SCHNEIDER, A.; NUNES, V. R. R.; SILVA, R. R. da; PFEIFER, L. F. M. |
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Afiliação: |
JÉSSICA DE SOUZA ANDRADE, PROGRAMA DE PÓS-GRADUAÇÃO EM BIODIVERSIDADE E BIOTECNOLOGIA; ELIZÂNGELA MÍRIAN MOREIRA, UNIVERSIDADE FEDERAL DO AMAZONAS; GEORGE MOREIRA DA SILVA, UNIVERSIDADE FEDERAL DE RONDÔNIA; AUGUSTO SCHNEIDER, UNIVERSIDADE FEDERAL DE PELOTAS; VANESSA RACHELE RIBEIRO NUNES, CPAF-RO; RENATA REIS DA SILVA, CPAF-RO; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO. |
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Título: |
Uterine health and fertility of timed AI postpartum Nelore beef cows raised in the Amazon biome. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Livestock Science, v. 249, 104528, July 2021. |
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ISSN: |
1871-1413 |
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DOI: |
https://doi.org/10.1016/j.livsci.2021.104528 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, transcription of IL-8 was greater (P = 0.02) in SCEP than HE and SCE groups. The relative transcription IL-1B was greater (P < 0.05) in SCEP than in PVD+, but the cows in the HE and SCE groups were not different from the other groups (P = 0.08). Our results demonstrate that postpartum beef cows, raised in the Amazon biome, with a higher proportion of PMN cells have lower fertility. Moreover, ~ 40% of the postpartum cows included in TAI programs early postpartum had suboptimal uterine conditions (subclinical endometritis, purulent vaginal discharge, or both). MenosThis study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, trans... Mostrar Tudo |
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Palavras-Chave: |
Citocina; Cytobrush; Inseminação artifical por tempo determinado; PMN cells; Timed artificial insemination (TAI); Uterine health. |
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Thesagro: |
Fertilidade Animal; Gado de Corte; Gado Nelore; Imunidade; Pós-Parto; Reprodução Animal; Sanidade Animal; Útero; Vaca de Corte. |
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Thesaurus Nal: |
Animal fertility; Animal reproduction; Beef cows; Cytokines; Immunity; Nellore; Postpartum period. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
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008 2021 bl uuuu u00u1 u #d
022 $a1871-1413
024 7 $ahttps://doi.org/10.1016/j.livsci.2021.104528$2DOI
100 1 $aANDRADE, J. de S.
245 $aUterine health and fertility of timed AI postpartum Nelore beef cows raised in the Amazon biome.$h[electronic resource]
260 $c2021
520 $aThis study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, transcription of IL-8 was greater (P = 0.02) in SCEP than HE and SCE groups. The relative transcription IL-1B was greater (P < 0.05) in SCEP than in PVD+, but the cows in the HE and SCE groups were not different from the other groups (P = 0.08). Our results demonstrate that postpartum beef cows, raised in the Amazon biome, with a higher proportion of PMN cells have lower fertility. Moreover, ~ 40% of the postpartum cows included in TAI programs early postpartum had suboptimal uterine conditions (subclinical endometritis, purulent vaginal discharge, or both).
650 $aAnimal fertility
650 $aAnimal reproduction
650 $aBeef cows
650 $aCytokines
650 $aImmunity
650 $aNellore
650 $aPostpartum period
650 $aFertilidade Animal
650 $aGado de Corte
650 $aGado Nelore
650 $aImunidade
650 $aPós-Parto
650 $aReprodução Animal
650 $aSanidade Animal
650 $aÚtero
650 $aVaca de Corte
653 $aCitocina
653 $aCytobrush
653 $aInseminação artifical por tempo determinado
653 $aPMN cells
653 $aTimed artificial insemination (TAI)
653 $aUterine health
700 1 $aMOREIRA, E. M.
700 1 $aSILVA, G. M. da
700 1 $aSCHNEIDER, A.
700 1 $aNUNES, V. R. R.
700 1 $aSILVA, R. R. da
700 1 $aPFEIFER, L. F. M.
773 $tLivestock Science$gv. 249, 104528, July 2021.
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Embrapa Rondônia (CPAF-RO) |
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| Registros recuperados : 26 | |
| 3. |  | COSTA, M. R. T. da R. A conservação do muçuã. In: GORAYEB, I. de S. (Coord.). Amazônia. Belém, PA, RM Graph: Jornal O Liberal, 2010. p. 126-127.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | NASCIMENTO, S. V. do; COSTA, M. R. T. da R.; OLIVEIRA, M. do S. P. de. Caracterização genética de acessos de açaizeiros (Euterpe oleracea) provenientes de Tomé-Açu, Pará, pro marcadores microssatélites. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 18.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 2., 2014, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2014. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|  |
| 8. | | SILVA, C. S.; COSTA, M. R. T. da R.; MARQUES, J. R. F.; VALENTE, J. V. Estimativas da variabilidade genética em bubalinos utilizando microssatélites. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 14., 2010, Belém, PA. Bolsista de iniciação científica: um aporte ao desenvolvimento da pesquisa agropecuária: anais. Belém, PA: Embrapa Amazônia Oriental, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 13. | | FORTES, A. C. R.; COSTA, M. R. T. da R.; BOARI, A. de J.; SILVA, C. S. Seleção de primares para análise molecular em genótipos de dendezeiro. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA, 14., 2010, Belém, PA. Bolsista de iniciação científica: um aporte ao desenvolvimento da pesquisa agropecuária: anais. Belém, PA: Embrapa Amazônia Oriental, 2010. 1 CD-ROM. PIBIC 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 16. | | DEGRANDI, T. M.; REIMCHE, R. B.; GUNSKI, R. J.; COSTA, M. R. T. da R.; MARQUES, J. R. F.; MARCONDES, C. R.; GARNERO, A. V. Citogenética aplicada a conservação de bubalinos na região Amazonica, Brasil. In: REUNIÃO BRASILEIRA DE CITOGENÉTICA, 2., 2011, Águas de Lindóia. Anais... Águas de Lindóia: Sociedade Brasileira de Genética, 2011. CAO28| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
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| 18. | | DEGRANDI, T. M.; MARQUES, J. R. F.; GUNSKI, R. J.; COSTA, M. R. T. da R.; MARQUES, L. C.; FIGUEIRO, M. R.; VINADÉ, L.; GARNERO, A. D. V. Cytogenetic identification of four generations of crossbred buffaloes maintained in a conservation program in the Marajó island/Brazil. Journal of Biotechnology and Biodiversity, Gurupi, v. 5, n. 2, p. 162-171, May 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | COSTA, M. R. T. da R.; BOARI, A. de J.; SILVA, C. S.; FORTES, A. C. R.; OLIVEIRA, M. do S. P. de; RODRIGUES, S. de M. Seleção de primers para análise genética do dendezeiro por marcadores moleculares. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. p. 510-511. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304). Editora técnica Clara Oliveira Goedert.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 20. | | LAMEIRA, O. A.; GIMENES, M. A.; NEVES, R. L. P.; OLIVEIRA, M. do S. P. de; RODRIGUES, S. de M.; DIONISIO, L. F. S.; COSTA, M. R. T. da R. Curauá genetic diversity in germplasm banks and natural populations. Journal of Plant Breeding and Crop Science, v. 10, n. 12, p. 347-353, Dec. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 26 | |
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