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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
10/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ASSIS, A. S. F.; OTENIO, M. H.; DRUMOND, B. P.; FUMIAN, T. M.; MIAGOSTOVICH, M. P.; SILVA, M. L. da R. e. |
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Afiliação: |
Andrêssa S.F.Assis, UFJF; MARCELO HENRIQUE OTENIO, CNPGL; Betânia P. Drumond, UFJF; Tulio M. Fumian, Instituto Oswaldo Cruz; Marize P Miagostovich, Instituto Oswaldo Cruz; Maria Luzia da Rosa e Silva, UFJF. |
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Título: |
Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Science of The Total Environment, v. 583, p. 163-168, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories. MenosAbstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really... Mostrar Tudo |
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Palavras-Chave: |
Human adenovirus; Internal control; Organic flocculation; Quantitative PCR; Wastewater treatment plant. |
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Thesaurus Nal: |
sewage sludge. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02366naa a2200253 a 4500
001 2084982
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
100 1 $aASSIS, A. S. F.
245 $aOptimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge.$h[electronic resource]
260 $c2017
520 $aAbstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories.
650 $asewage sludge
653 $aHuman adenovirus
653 $aInternal control
653 $aOrganic flocculation
653 $aQuantitative PCR
653 $aWastewater treatment plant
700 1 $aOTENIO, M. H.
700 1 $aDRUMOND, B. P.
700 1 $aFUMIAN, T. M.
700 1 $aMIAGOSTOVICH, M. P.
700 1 $aSILVA, M. L. da R. e
773 $tScience of The Total Environment$gv. 583, p. 163-168, 2017.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 12 | |
| 4. |  | DALAGNOL, L. M. G.; BRUTOLIN, J. G.; COMACHIO, V.; CAMARGO, U. A.; MAIA, J. D. G.; RITSCHEL, P. S. Incidência das principais doenças da videira em acessos mantidos pelo Banco Ativo de Germoplasma de Uva (BAG-Uva). In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 11.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 7., Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2013. p. 55. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
|    |
| 5. | | BRUSTOLIN, J. G.; FONTANA, V.; COMACHIO, V.; TONIETTO, J.; FERREIRA, M. E.; MAIA, J. D. G.; RITSCHEL, P. S. Caracterização genética, fenotípica e sensorial da cultivar de videira 'Moscato Branco'. In.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2015. p. 56.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Uva e Vinho. |
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| 7. | | FURINI, G.; REINEHR, J.; COMACHIO, V.; ZILIO, R. A.; MAIA, J. D. G.; SOUZA, R. T. de; RITSCHEL, P. S. Atributos visuais e sensoriais de uvas de mesa cultivadas sob cobertura plástica na Serra Gaúcha. In.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2015. p. 62.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | REINEHR, J.; FURINI, G.; COMACHIO, V.; ZILIO, R. A.; TAFFAREL, J. C.; MAIA, J. D. G.; RITSCHEL, P. S. Avaliação de atributos físico-químicos e sensoriais de sucos de uva elaborados com nove cultivares de uva. In:CONGRESSO LATINO-AMERICANO DE VITICULTURA E ENOLOGIA, 15., 2015, Bento Gonçalves, RS. p. 379. XV Congresso Latino-Americano de Viticultura e Enologia E XIII Congresso Brasileiro de Viticultura e Enologia. Bento Gonçalves-RS, 3 a 7 de Novembro de 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 9. | | DALAGNOL, L. M. G.; CAUMO, M.; POLONI, T.; COMACHIO, V.; CAMARGO, U. A.; MAIA, J. D. G.; RITSCHEL, P. S. Características do mosto e de compostos relacionados à saúde de acessos mantidos pelo Banco Ativo de Germoplasma de Uva (BAGUva). In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 11.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 7., Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2013. p. 45. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 10. | | DALAGNOL, L. M. G.; LORENZINI, M.; COMACHIO, V.; CAMARGO, U. A.; MAIA, J. D. G.; RITSCHEL, P. S. Avaliações agronômicas de acessos mantidos pelo Banco Ativo de Germoplasma de Uva (BAG-Uva). In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 11.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 7., Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2013. p. 54. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 11. | | BOSCO, D. D.; SINSKI, I.; COMACHIO, V.; MAIA, J. D. G.; FAJARDO, T. V. M.; RITSCHEL, P. S.; QUECINI, V. In vitro establishment and conservation of grapevine germplasm. In: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...] [S.l.]: International Crop Science Society, 2012. Não paginado. 1 pendrive. Resumo 3096.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 12. | | REINEHR, J.; FURINI, G.; LIMA, J. de; ROSSETTO, D.; COMACHIO, V.; ZILIO, R. A.; TAFFAREL, J. C.; MAIA, J. D. G.; RITSCHEL, P. S. Avaliação físico-química e sensorial de sucos elaborados com diferentes cultivares de uva. In.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2015. p. 63.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 12 | |
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