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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
19/02/2019 |
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Data da última atualização: |
19/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLETI, M. D.; REGITANO, L. C. de A.; SOUSA, G. H. M. F.; CESAR, A. S. M.; SIMAS, R. C.; SILVA-VIGNATO, B.; OLIVEIRA, G. B.; ANDRADE, S. C. S.; CAMERON, L. C.; COUTINHO, L. L. |
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Afiliação: |
Mirele D. Poleti, USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; Gustavo H. M. F. Souza, MS Applications and Development Laboratory Waters Corporation; Aline S. M. Cesar, USP; Rosineide C. Simas, USP; Bárbara Silva-Vignato, USP; Gabriella B. Oliveira, USP; Sónia C. S. Andrade, USP; Luiz C. Cameron, UNIRIO; Luiz Lehmann Coutinho, USP. |
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Título: |
Longissimus dorsi muscle label-free quantitative proteomic reveals biological mechanisms associated with intramuscular fat deposition. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 179, p. 30-41, 2018. |
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DOI: |
https://doi.org/10.1016/j.dib.2018.06.004 |
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Idioma: |
Inglês |
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Conteúdo: |
The pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in human health and meat quality attributes, influencing energy metabolism of skeletal muscle, as well as, tenderness, flavor, and juiciness of beef. We performed for the first time the utilization of integrated transcriptome-assisted label-free quantitative proteomic approach using High Definition Mass Spectrometry for characterization of the changes in the proteomic profile of the Longissimus dorsi muscle associated with intramuscular fat deposition in cattle. Furthermore, we compared the muscle proteome with the muscle transcriptome (mRNA and microRNAs), obtained by RNA-sequencing, to better understand the relationship between expression of mRNAs and proteins and to unravel essential biological mechanisms involved in bovine skeletal muscle IMF deposition. MenosThe pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in... Mostrar Tudo |
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Palavras-Chave: |
Adiposidade; Gordura intramuscular; MRNA. |
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Thesagro: |
Gordura Animal. |
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Thesaurus Nal: |
Mass spectrometry; MicroRNA. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03231naa a2200313 a 4500
001 2106242
005 2019-02-19
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.dib.2018.06.004$2DOI
100 1 $aPOLETI, M. D.
245 $aLongissimus dorsi muscle label-free quantitative proteomic reveals biological mechanisms associated with intramuscular fat deposition.$h[electronic resource]
260 $c2018
520 $aThe pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in human health and meat quality attributes, influencing energy metabolism of skeletal muscle, as well as, tenderness, flavor, and juiciness of beef. We performed for the first time the utilization of integrated transcriptome-assisted label-free quantitative proteomic approach using High Definition Mass Spectrometry for characterization of the changes in the proteomic profile of the Longissimus dorsi muscle associated with intramuscular fat deposition in cattle. Furthermore, we compared the muscle proteome with the muscle transcriptome (mRNA and microRNAs), obtained by RNA-sequencing, to better understand the relationship between expression of mRNAs and proteins and to unravel essential biological mechanisms involved in bovine skeletal muscle IMF deposition.
650 $aMass spectrometry
650 $aMicroRNA
650 $aGordura Animal
653 $aAdiposidade
653 $aGordura intramuscular
653 $aMRNA
700 1 $aREGITANO, L. C. de A.
700 1 $aSOUSA, G. H. M. F.
700 1 $aCESAR, A. S. M.
700 1 $aSIMAS, R. C.
700 1 $aSILVA-VIGNATO, B.
700 1 $aOLIVEIRA, G. B.
700 1 $aANDRADE, S. C. S.
700 1 $aCAMERON, L. C.
700 1 $aCOUTINHO, L. L.
773 $tJournal of Proteomics$gv. 179, p. 30-41, 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 128 | |
| 6. |  | COOPER, M.; CASTRO, S. S. de; COELHO, M. R. Micromorfologia do solo. In: TEIXEIRA, P. C.; DONAGEMMA, G. K.; FONTANA, A.; TEIXEIRA, W. G. (Ed.). Manual de métodos de análise de solo. 3. ed. rev. e ampl. Brasília, DF: Embrapa, 2017. pt. 5, cap. 1, p. 527-564.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Solos. |
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| 7. | | MONIZ, A. C.; SANTOS, H. G. dos; CASTRO, S. S.; AGENA, S. S. Atributos de Latossolos remanescentes em áreas onde atualmente ocorre o processo de bissialitização, em Búzios (RJ). In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 27., 1999, Brasília, DF. Resumos [...]. Planaltina, DF: Embrapa Cerrados, 1999. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 10. | | CASTRO, S. F.; BERTECHINI, A. G.; TEIXEIRA, L. V.; MAZZUCO, H.; AMORIM, B. G. Improved prediction of amino acids digestibility and metabolizable energy content in soybean meal and full-fat soybean for broilers. In: PSA ANNUAL MEETING, 2014, Corpus Christi. Abstracts... Champaign: Poultry Science Association, 2014. p. 113. Publicado em: Poultry Science, v. 93, E-Suppl. 1, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 11. | | CASTRO, S. T. R.; BIAZIO, G. R. de; LEDUR, M. C.; PAIVA, S. R. Caracterização molecular de galinhas (Gallus gallus). In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Amazônia: recursos genéticos e sustentabilidade: anais. Brasília, DF: SBRG; Belém, PA: Embrapa Amazônia Oriental, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | CASTRO, S. T. R.; BIAZIO, G. R. de; LEDUR, M. C.; PAIVA, S. R. Caracterização molecular de galinhas (Gallus gallus). In: CONGRESSO NACIONAL DE BOTÂNICA, 73., 2023, Belém, PA. A Botânica no Brasil: desafios interdisciplinares e interculturais: anais. Belém, PA: Sociedade Botânica do Brasil, 2023.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 16. | | FRIGHETTO, R. T. S.; VALARINI, P. J.; RIZZO, LUIZ T. B.; CASTRO, S. S. distribuição espacial e temporal do teor de carbono em polissacarídeos, no horizonte de solos agrícolas: estudos de caso. In: ENCONTRO BRASILEIRO SOBRE SUBSTÂNCIAS HÚMICAS, 3., 1999, Santa Maria, RS. Anais...Matéria Orgânica e qualidade Ambiente:Universidade Federal de Santa Maria, Santa Maria, 1999. p.352-354| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 17. | | COUTINHO, H. L.; TURETTA, A. P. D.; MONTEIRO, J. M.; CASTRO, S. S.; MALHEIROS, T. F. Avaliação da sustentabilidade das mudanças de uso da terra ocasionadas pela expansão da cana-de-açúcar no Brasil Central. In: WORKSHOP INTERDISCIPLINAR DE PESQUISA EM INDICADORES DE SUSTENTABILIDADE, 3., 2011, São Carlos, SP. Indicadores e avaliação de sustentabilidade: ferramentas, propostas e desafios para a Conferência das Nações Unidas sobre Desenvolvimento Sustentável Rio+20: anais de resumos. São Carlos, SP: EESC/FSP/USP, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 18. | | TURETTA, A. P. D.; CASTRO, S. S. de; POLIDORO, J. C. Solos, sustentabilidade e provisão de serviços ecossistêmicos. Boletim Informativo da Sociedade Brasileira de Ciência do Solo, Viçosa, MG, v. 43, n. 2, p. 26-30, maio/ago. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Solos. |
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| 19. | | RAMOS, A. F.; AZEVEDO, H. C.; CASTRO, S. T. R.; EGITO, A. A. do. Manual de curadores de germoplasma - animal: conservação ex situ/in vitro de sêmen, embriões, DNA e tecidos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2012. 18 p. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 340; Embrapa Tabuleiros Costeiros. Documentos, 176).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 128 | |
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