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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/02/2024 |
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Data da última atualização: |
08/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CUNHA, T.; MIRANDA, M. P.; ZANARDI, O. Z.; MONNERAT, R.; MARQUES, J. P. R.; DORTA, S. O.; MACEDO, C. L.; MACHADO, M. A.; ASTUA, J. de F. |
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Afiliação: |
TATIANE CUNHA, UNIVERSIDADE DE SÃO PAULO; MARCELO P. MIRANDA, FUNDO DE DEFESA DA CITRICULTURA (FUNDECITRUS); ODIMAR Z. ZANARDI, INSTITUTO FEDERAL DE SANTA CATARINA; ROSE MONNERAT, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; JOÃO P.R. MARQUES, UNIVERSITY OF SÃO PAULO; SÍLVIA O. DORTA, UNIVERSIDADE DE SÃO PAULO; CRISTINA L. MACEDO, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; MARCOS A. MACHADO, CENTRO DE CITRICULTURA SYLVIO MOREIRA; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Bacillus thuringiensis translocation in citrus scion/rootstock combinations and binding of cry toxins with the Asian citrus psyllid gut receptors. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Journal Pre-proof, Crop Protection, 2024. |
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Páginas: |
46 p. |
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DOI: |
https://doi.org/10.1016/j.cropro.2024.106593 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Bacillus thuringiensis (Bt) can be effective in controlling insect pests either in spray products or by their toxins expressed in transgenic crops. The discovery of Bt as endophytic in citrus opened new perspectives for the control of Diaphorina citri Kuwayama, the vector of the bacteria Candidatus Liberibacter spp. associated with Huanglongbing. In this study, the endophytic translocation of Bt in citrus seedlings (small and large plants) and in different scion-rootstock combinations were investigated. We also assessed the lethal concentration of D. citri nymphs caused by systemic Bt in citrus and, moreover, the binding of Cry toxins with the Asian citrus psyllid (ACP) gut receptors. Endophytic translocation of Bt in citrus seedlings and nursery trees was confirmed by colony isolation, PCR detection, and Btk-GFP microscopy analyses, which demonstrated that, after Bt inoculation into the substrate, the bacteria enter through lateral root emergence sites and are localized in stem xylem vessel elements. Mean lethal concentrations for D. citri third instar nymphs were 4.92 × 104 and 2.19 × 104 spores mL−1 for S1450 and S1302 strains, respectively. Nymphal mortality induced by the bacteria was higher in citrus seedlings than in citrus nursery trees. All Cry-toxins tested were able to bind to D. citri brush border membrane vesicles, suggesting the potential pathogenicity of Bt strains against psyllid. This study clearly demonstrates the penetration and Bt translocation in the xylem of small and large citrus plants and the potential of the bacteria to cause ACP nymphs mortality that fed on shoots from Bt-treated citrus. MenosAbstract: Bacillus thuringiensis (Bt) can be effective in controlling insect pests either in spray products or by their toxins expressed in transgenic crops. The discovery of Bt as endophytic in citrus opened new perspectives for the control of Diaphorina citri Kuwayama, the vector of the bacteria Candidatus Liberibacter spp. associated with Huanglongbing. In this study, the endophytic translocation of Bt in citrus seedlings (small and large plants) and in different scion-rootstock combinations were investigated. We also assessed the lethal concentration of D. citri nymphs caused by systemic Bt in citrus and, moreover, the binding of Cry toxins with the Asian citrus psyllid (ACP) gut receptors. Endophytic translocation of Bt in citrus seedlings and nursery trees was confirmed by colony isolation, PCR detection, and Btk-GFP microscopy analyses, which demonstrated that, after Bt inoculation into the substrate, the bacteria enter through lateral root emergence sites and are localized in stem xylem vessel elements. Mean lethal concentrations for D. citri third instar nymphs were 4.92 × 104 and 2.19 × 104 spores mL−1 for S1450 and S1302 strains, respectively. Nymphal mortality induced by the bacteria was higher in citrus seedlings than in citrus nursery trees. All Cry-toxins tested were able to bind to D. citri brush border membrane vesicles, suggesting the potential pathogenicity of Bt strains against psyllid. This study clearly demonstrates the penetration and Bt translocation ... Mostrar Tudo |
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Thesagro: |
Porta Enxerto; Translocação. |
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Thesaurus Nal: |
Brush border membrane vesicles; Citrus; Diaphorina citri; Endotoxins. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02611naa a2200313 a 4500
001 2161812
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008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cropro.2024.106593$2DOI
100 1 $aCUNHA, T.
245 $aBacillus thuringiensis translocation in citrus scion/rootstock combinations and binding of cry toxins with the Asian citrus psyllid gut receptors.$h[electronic resource]
260 $c2024
300 $a46 p.
520 $aAbstract: Bacillus thuringiensis (Bt) can be effective in controlling insect pests either in spray products or by their toxins expressed in transgenic crops. The discovery of Bt as endophytic in citrus opened new perspectives for the control of Diaphorina citri Kuwayama, the vector of the bacteria Candidatus Liberibacter spp. associated with Huanglongbing. In this study, the endophytic translocation of Bt in citrus seedlings (small and large plants) and in different scion-rootstock combinations were investigated. We also assessed the lethal concentration of D. citri nymphs caused by systemic Bt in citrus and, moreover, the binding of Cry toxins with the Asian citrus psyllid (ACP) gut receptors. Endophytic translocation of Bt in citrus seedlings and nursery trees was confirmed by colony isolation, PCR detection, and Btk-GFP microscopy analyses, which demonstrated that, after Bt inoculation into the substrate, the bacteria enter through lateral root emergence sites and are localized in stem xylem vessel elements. Mean lethal concentrations for D. citri third instar nymphs were 4.92 × 104 and 2.19 × 104 spores mL−1 for S1450 and S1302 strains, respectively. Nymphal mortality induced by the bacteria was higher in citrus seedlings than in citrus nursery trees. All Cry-toxins tested were able to bind to D. citri brush border membrane vesicles, suggesting the potential pathogenicity of Bt strains against psyllid. This study clearly demonstrates the penetration and Bt translocation in the xylem of small and large citrus plants and the potential of the bacteria to cause ACP nymphs mortality that fed on shoots from Bt-treated citrus.
650 $aBrush border membrane vesicles
650 $aCitrus
650 $aDiaphorina citri
650 $aEndotoxins
650 $aPorta Enxerto
650 $aTranslocação
700 1 $aMIRANDA, M. P.
700 1 $aZANARDI, O. Z.
700 1 $aMONNERAT, R.
700 1 $aMARQUES, J. P. R.
700 1 $aDORTA, S. O.
700 1 $aMACEDO, C. L.
700 1 $aMACHADO, M. A.
700 1 $aASTUA, J. de F.
773 $tJournal Pre-proof, Crop Protection, 2024.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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| Registros recuperados : 15 | |
| 1. |  | CANAL, C. W.; VAZ, C. S. L. Vacinas víricas. In: FLORES, E. F. (Org.). Virologia veterinária. Santa Maria: Ed. da UFSM, 2007. p.329-358. Projeto: 16.00.30004-00| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 2. | | CANAL, C. W.; VAZ, C. S. L. Vacinas víricas. In: FLORES, E. F. (Org.). Virologia veterinária: virologia geral e doenças víricas. 2. ed. Santa Maria: UFSM, 2012. p. 369-398 Projeto/Plano de Ação: 03.08.06.004.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 4. | | SOUZA, C. K. de; IKUTA, N.; STRECK, A. F.; CORBELLINI, A. O.; GAVA, D.; PINTO, L. D.; CANAL, C. W. Detection and quantification of porcine parvovirus 1 using a taqman-based real-time PCR for NS1 gene. In: NATIONAL MEETING OF VIROLOGY, 22.; MERCOSUR MEETING OF VIROLOGY, 6., 2011, Itibaia. Anais... Itibaia: SBV, 2011. Vírus Reviews and Research, n. 16, supl. 1, oct. 2011. p. 269. Projeto/Plano de Ação: 11.11.11.111.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
|  |
| 5. | | GAVA, D.; SOUZA, C. K. de; CANAL, C. W.; ZIMMERMAN, J.; CORBELLINI, L. G.; BORTOLOZZO, F. P.; WENZ, I. Comparison of Hemagglutination inhibition test and Elisa to detect porcine parvovirus antibodies. In: NATIONAL MEETING OF VIROLOGY, 22.; MERCOSUR MEETING OF VIROLOGY, 6., 2011, Itibaia. Anais... Itibaia: SBV, 2011. Vírus Reviews and Research, n. 16, supl. 1, oct. 2011. p. 269. Projeto/Plano de Ação: 11.11.11.111.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 6. | | VAZ, C. S. L.; STRECK, A. F.; MICHAEL, G. B.; MARKS, F. S.; RODRIGUES, D. P.; REIS, E. M. F. dos; CARDOSO, M. R. I.; CANAL, C. W. Antimicrobial resistance and subtyping of Salmonella enterica subspecies enterica serovar Enteritidis isolated from human outbreaks and poultry in southern Brazil. Poultry Science, v. 89, p. 1530-1536, 2010. Projeto/Plano de Ação: 03.08.60.400-03.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | VAZ, C. S. L.; RODRIGUES, D. P.; REIS, E. M. F.; MARKS, F. S.; STRECK, A. F.; CARDOSO, M. R. L.; CANAL, C. W. Molecular and phenotypic characterization of Salmonella enteritidis isolated from salmonellosis outbreaks and poultry products in southern Brazil. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24., 2007, Brasília, DF. Anais. Brasília, DF: Sociedade Brasileira de Microbiologia, 2007. 1 CD-ROM. Projeto n. 16.00.300.01-16| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | GAVA, D.; STRECK, A. F.; SOUZA, C. K.; GONÇALVES, K. R.; ZANELLA, J. R. C.; WENTZ, I.; BORTOLOZZO, F. P.; CANAL, C. W. Detecção do parvovírus suíno no soro de fêmeas suínas de acordo com a ordem de parto através de nested-PCR. In: FÓRUM INTERNACIONAL DA SUINOCULTURA, 4., 2008. Curitiba. Trabalhos técnicos: anais. Concórdia: Embrapa Suínos e Aves, 2008. p. 76-77. Projeto/Plano de Ação: 03.07.51.900-02.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 9. | | SOUZA, C. K. de S.; GAVA, D.; MOSENA, A. C. S.; BIONDO, N.; SCHAEFER, R.; ZANELLA, J. R. C.; BARCELLOS, D. E. S. N. de; CANAL, C. W. Detection of emerging parvoviruses and anellovirus in lung of captive wild boars. In: BRAZILIAN CONGRESS OF VIROLOGY, 23.; MERCOSUR MEETING OF VIROLOGY, 7., 2012, Foz do Iguaçu. Virus and Research? Foz do Iguaçu: SBV, 2012. p.425-426. Journal of the Brazilian Society for Virology, v. 17, n. 2, suppl. 1, 2012. Projeto/Plano de Ação: 02.11.10.600-03.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 10. | | SOUZA, C. K. de; STRECK, A. F.; GAVA, D.; CORBELINNI, A. O.; MATA, H.; PINTO, L. da S.; GONÇALVES, K. R.; CANAL, C. W. Detection and phylogenetic analysis of porcine hokovirus in piglets with postweaning multisystemic wasting syndrome. In: NATIONAL MEETING OF VIROLOGY, 22.; MERCOSUR MEETING OF VIROLOGY, 6., 2011, Itibaia. Anais... Itibaia: SBV, 2011. Vírus Reviews and Research, n. 16, supl. 1, oct. 2011. p. 268. Projeto/Plano de Ação: 11.11.11.111.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 11. | | MARKS, F. S.; RODENBUSCH, C. R.; OKINO, C. H.; HEIN, H. E.; COSTA, E. de F.; MACHADO, G.; CANAL, C. W.; BRENTANO, L.; CORBELLINI, L. G. Targeted survey of newcastle disease virus in backyard poultry flocks located in wintering site for migratory birds from southern Brazil. Preventive Veterinary Medicine, St. Louis, v. 116, p.197-202, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 12. | | GAVA, D.; ARGENTI, L. E.; MORES, T. J.; SOUZA, C. K.; STRECK, A. F.; BERNARDI, M. L.; CANAL, C. W.; BORTOLOZZO, F. P.; WENTZ, I. Caracterização do perfil sorológico de nulíparas suínas e da progênie frente ao parvovírus suíno. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 15., 2011, Fortaleza. Anais de palestras e resumos. Fortaleza: Abraves, 2011. 1 CD-ROM. Projeto: 03.09.00.046.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 13. | | SOUZA, C. K.; OLDIGES, D. P.; VAZ JUNIOR, I. da; CORBELLINI, L. G.; SCHAEFER, R.; GAVA, D.; ZANELLA, J. R. C.; CANAL, C. W. Indirect Elisa using a recombinant nucleoprotein for Influenza a vírus antibody detection in swine. In: CONGRESSO BRASILEIRO DE VIROLOGIA, 28.; ENCONTRO DE VIROLOGIA DO MERCOSUL, 12., 2017, Belo Horizonte. Trabalho pôsteres... Brasília: SBV, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 14. | | VIANCELLI, A.; KUNZ, A.; STEINMETZ, R. L. R.; KICH, J. D.; SOUZA, C. K. de; CANAL C. W.; COLDEBELLA, A.; ESTEVES, P. A.; BARARDI, C. R. M. Performance of two swine manure treatment systems on chemical composition and on the reduction of pathogens. Chemosphere, Oxford, v. 90, p. 1539-1544, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 15. | | SOUZA, C. K.; OLDIGES, D. P.; POETA, A. P. S.; VAZ JÚNIOR, I. S.; SCHAEFER, R.; GAVA, D.; ZANELLA, J. R. C.; CANAL, C. W.; CORBELLINI, L. G. Serological surveillance and factors associated with influenza A virus in backyard pigs in Southern Brazil. Zoonoses Public Health, p. 1-8, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 15 | |
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