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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/05/2020 |
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Data da última atualização: |
02/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRITO, F. S. D.; SANTOS, J. R. P.; AZEVEDO, V. C. R.; PEIXOUTO, Y. S.; OLIVEIRA, S. A. S. de; FERREIRA, C. F.; HADDAD, F.; AMORIM, E. P.; FRAAIJE, B.; MILLER, R. N. G. |
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Afiliação: |
FABIANE S. D. BRITO; JANSEN R. P. SANTOS; VÂNIA C. R. AZEVEDO; YSLAI S. PEIXOUTO; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF; FERNANDO HADDAD, CNPMF; EDSON PERITO AMORIM, CNPMF; BART FRAAIJE; ROBERT N. G. MILLER. |
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Título: |
Genetic Diversity and Azole fungicide sensitivity in pseudocercospora musae field populations in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Microbiology, v, 11, n.99, 2020. |
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ISSN: |
1664-302X |
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DOI: |
10.3389/fmicb.2020.00099 |
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Idioma: |
Inglês |
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Conteúdo: |
Pseudocercospora musae, causal agent of Sigatoka leaf spot, or yellow Sigatoka disease, is considered a major pathogen of banana (Musa spp.). Widely disseminated in Brazil, this study explored the genetic diversity in field populations of the pathogen from production areas in the Distrito Federal and the States of Bahia, Minas Gerais, and Rio Grande do Norte. Resistance to demethylation inhibitor (DMI) fungicides was also examined. For 162 isolates from 10 banana growing regions, analysis of mating type idiomorph frequency was conducted, together with estimation of genetic diversity at 15 microsatellite loci. A total of 149 haplotypes were identified across the examined populations, with an average genetic diversity of 4.06. In general, populations displayed 1:1 proportions of idiomorphs MAT1-1 and MAT1-2, providing evidence for sexual recombination. Multilocus linkage disequilibrium also indicated asexual reproduction contributing to the genetic structure of certain populations. AMOVA revealed that 86.3% of the genetic differentiation of the pathogen occurred among isolates within populations. Discriminant Analysis of Principal Components (DAPC) identified six most probable genetic groups, with no population structure associated with geographic origin or collection site. Although genetic similarity was observed among certain populations from different states, data revealed increasing genetic differentiation with increasing geographic distance, as validated by Mantel?s test (r = 0.19, P < 0.001). On the basis of DMI fungicide sensitivity testing and CYP51 gene sequence polymorphism, isolates from the Distrito Federal separated into two main groups, one with generally higher EC50 values against eight DMI fungicides. A clear phenotype-to-genotype relationship was observed for isolates carrying the CYP51 alteration Y461N. Conventionally adopted fungicides for control of Sigatoka leaf spot are likely to be overcome by combined sexual and asexual reproduction mechanisms in P. musae driving genetic variability. Continued analysis of pathogen genetic diversity and monitoring of DMI sensitivity profiles of Brazilian field populations is essential for the development of integrated control strategies based on host resistance breeding and rational design of fungicide regimes. MenosPseudocercospora musae, causal agent of Sigatoka leaf spot, or yellow Sigatoka disease, is considered a major pathogen of banana (Musa spp.). Widely disseminated in Brazil, this study explored the genetic diversity in field populations of the pathogen from production areas in the Distrito Federal and the States of Bahia, Minas Gerais, and Rio Grande do Norte. Resistance to demethylation inhibitor (DMI) fungicides was also examined. For 162 isolates from 10 banana growing regions, analysis of mating type idiomorph frequency was conducted, together with estimation of genetic diversity at 15 microsatellite loci. A total of 149 haplotypes were identified across the examined populations, with an average genetic diversity of 4.06. In general, populations displayed 1:1 proportions of idiomorphs MAT1-1 and MAT1-2, providing evidence for sexual recombination. Multilocus linkage disequilibrium also indicated asexual reproduction contributing to the genetic structure of certain populations. AMOVA revealed that 86.3% of the genetic differentiation of the pathogen occurred among isolates within populations. Discriminant Analysis of Principal Components (DAPC) identified six most probable genetic groups, with no population structure associated with geographic origin or collection site. Although genetic similarity was observed among certain populations from different states, data revealed increasing genetic differentiation with increasing geographic distance, as validated by Mantel?s test ... Mostrar Tudo |
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Thesagro: |
Banana; Sigatoka Negra. |
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Categoria do assunto: |
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Marc: |
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024 7 $a10.3389/fmicb.2020.00099$2DOI
100 1 $aBRITO, F. S. D.
245 $aGenetic Diversity and Azole fungicide sensitivity in pseudocercospora musae field populations in Brazil.$h[electronic resource]
260 $c2020
520 $aPseudocercospora musae, causal agent of Sigatoka leaf spot, or yellow Sigatoka disease, is considered a major pathogen of banana (Musa spp.). Widely disseminated in Brazil, this study explored the genetic diversity in field populations of the pathogen from production areas in the Distrito Federal and the States of Bahia, Minas Gerais, and Rio Grande do Norte. Resistance to demethylation inhibitor (DMI) fungicides was also examined. For 162 isolates from 10 banana growing regions, analysis of mating type idiomorph frequency was conducted, together with estimation of genetic diversity at 15 microsatellite loci. A total of 149 haplotypes were identified across the examined populations, with an average genetic diversity of 4.06. In general, populations displayed 1:1 proportions of idiomorphs MAT1-1 and MAT1-2, providing evidence for sexual recombination. Multilocus linkage disequilibrium also indicated asexual reproduction contributing to the genetic structure of certain populations. AMOVA revealed that 86.3% of the genetic differentiation of the pathogen occurred among isolates within populations. Discriminant Analysis of Principal Components (DAPC) identified six most probable genetic groups, with no population structure associated with geographic origin or collection site. Although genetic similarity was observed among certain populations from different states, data revealed increasing genetic differentiation with increasing geographic distance, as validated by Mantel?s test (r = 0.19, P < 0.001). On the basis of DMI fungicide sensitivity testing and CYP51 gene sequence polymorphism, isolates from the Distrito Federal separated into two main groups, one with generally higher EC50 values against eight DMI fungicides. A clear phenotype-to-genotype relationship was observed for isolates carrying the CYP51 alteration Y461N. Conventionally adopted fungicides for control of Sigatoka leaf spot are likely to be overcome by combined sexual and asexual reproduction mechanisms in P. musae driving genetic variability. Continued analysis of pathogen genetic diversity and monitoring of DMI sensitivity profiles of Brazilian field populations is essential for the development of integrated control strategies based on host resistance breeding and rational design of fungicide regimes.
650 $aBanana
650 $aSigatoka Negra
700 1 $aSANTOS, J. R. P.
700 1 $aAZEVEDO, V. C. R.
700 1 $aPEIXOUTO, Y. S.
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aFERREIRA, C. F.
700 1 $aHADDAD, F.
700 1 $aAMORIM, E. P.
700 1 $aFRAAIJE, B.
700 1 $aMILLER, R. N. G.
773 $tFrontiers in Microbiology, v, 11$gn.99, 2020.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 9 | |
| 1. |  | AMANCIO, C. O. da G.; AMANCIO, R.; TONIAZZO, R. C.; BOTELHO, D.; PELLEGRIN, L. A. Caracterização socioeconômica da comunidade do Amolar, sub-região do Paraguai, Corumbá, MS. Corumbá: Embrapa Pantanal, 2010. 4 p. (Embrapa Pantanal. Circular Técnica, 92).| Biblioteca(s): Embrapa Pantanal. |
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| 2. | | AMÂNCIO, C. O. da G.; AMÂNCIO, R.; TONIAZZO, R. C.; BOTELHO, D.; PELLEGRIN, L. A. Caracterização socioeconômica das comunidades Chané/Bonfim, sub-região do Paraguai, Corumbá, MS. Corumbá: Embrapa Pantanal, 2008. 9 p. (Embrapa Pantanal. Circular Técnica, 82).| Biblioteca(s): Embrapa Pantanal. |
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| 3. | | BOTELHO, C. E.; NAZARENO, A. G. M.; BARTHOLO, G. F.; BOTELHO, D. M. S.; NOGUEIRA, A. M. Avaliação da resistência à ferrugem em progênies de cafeeiro F4 obtidas por cruzamentos de 'icatu' com catimor. In: Simpósio de Pesquisa dos Cafés do Brasil, 5., 2007, Águas de Lindóia. Anais. Brasília, D.F. : Embrapa Café, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 4. | | BOTELHO, D. de O.; SILVA, S. S. da; AMÂNCIO, R.; PEREIRA, J. R.; AMÂNCIO, C. O. da G. Icms-ecológico como instrumento de política ambiental em Minas Gerais. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 45., 2007, Londrina. Conhecimentos para a agricultura do futuro. Brasília, DF: Sober; Londrina: Universidade Estadual de Londrina: IAPAR, 2007. 21 p. Disponível no CD2008.00008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pantanal. |
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| 5. | | BOTELHO, D. M. S.; RESENDE, M. L. V.; RIBEIRO JUNIOR, P. M.; PATRÍCIO, F. R. A.; VASCONCELOS, V. A. M.; PEREIRA, E. A. Evaluation of two methods for preservation of Cercospora coffeicola. In: INTERNATIONAL CONFERENCE ON COFFEE & SCIENCE, 25., 2014, Colombia. Leveraging Knowledge for coffee sustainability. Colombia: Association for Science and Information on Coffee (ASIC), 2014. p. 259.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 8. | | SERA, G. H.; CARVALHO, C. H. S. de; ABRAHÃO, J. C. de R.; POZZA, E. A.; MATIELLO, J. B.; ALMEIDA, S. R. de; BARTELEGA, L.; BOTELHO, D. M. dos S. Coffee leaf rust in Brazil: historical events, current situation, and control measures. Agronomy, v. 12, n. 2, 2022. 19 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
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| 9. | | BOTELHO, D. M. S.; RESENDE, M. L. de; RIBEIRO JUNIOR, P. M.; OLIVEIRA, A. C. B. de; PEREIRA, A. A.; OGOSHI, C.; VALE, P. A. S.; VASCONCELOS, V. A. M. Resistência de cultivares de cafeeiro a cercosporiose. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 48.; CONGRESSO BRASILEIRO DE PATOLOGIA PÓS-COLHEITA, 2., 2015, São Pedro. Fitopatologia de precisão: fronteiras da ciência: anais. Brasília, DF: Sociedade Brasileira de Fitopatologia, 2015. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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