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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
17/12/2014 |
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Data da última atualização: |
17/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALVAREZ-PIZARRO, J. C.; GOMES FILHO, E.; PRISCO, J. T.; GROSSI DE SA, M. F.; OLIVEIRA NETO, O. B. de; FRAGOSO, R. da R. |
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Afiliação: |
MARIA FATIMA GROSSI DE SA, CENARGEN; RODRIGO DA ROCHA FRAGOSO, CPAC. |
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Título: |
Plasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Biologia Plantarum, v. 58, n. 3, p. 507-514, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: We studied the influence of inorganic nitrogen sources (NO3- or NH4+) and potassium deficiency on expression and activity of plasma membrane (PM) H+-ATPase in sorghum roots. After 15 d of cultivation at 0.2 mM K+, the plants were transferred to solutions lacking K+ for 2 d. Then, K+ depletion assays were performed in the presence or absence of vanadate. Further, PMs from K+-starved roots were extracted and used for the kinetic characterization of ATP hydrolytic activity and the immunodetection of PM H+-ATPase. Two major genes coding PM H+-ATPase (SBA1 and SBA2) were analyzed by real-time PCR. PM H+-ATPase exhibited a higher Vmax and Km in NH4+-fed roots compared with NO3--fed roots. The optimum pH of the enzyme was slightly lower in NO3- -fed roots than in NH4+ -fed roots. The vanadate sensitivity was similar. The expressions of SBA1 and SBA2 were increased in roots grown under NH4+. Concomitantly, an increased content of the enzyme in PM was observed. The initial rate of K+ uptake did not differ between plants grown with NO3- or NH4+, but it was significantly reduced by vanadate in NH4+-grown plants. |
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Palavras-Chave: |
Nitrato de Amonia. |
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Thesagro: |
Fertilizante nitrogenado; Potássio; Sorghum Bicolor; Sorgo. |
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Thesaurus Nal: |
Ammonium nitrate; Nitrate fertilizers; Potassium. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 01933naa a2200277 a 4500
001 2003022
005 2014-12-17
008 2014 bl uuuu u00u1 u #d
100 1 $aALVAREZ-PIZARRO, J. C.
245 $aPlasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources.
260 $c2014
520 $aAbstract: We studied the influence of inorganic nitrogen sources (NO3- or NH4+) and potassium deficiency on expression and activity of plasma membrane (PM) H+-ATPase in sorghum roots. After 15 d of cultivation at 0.2 mM K+, the plants were transferred to solutions lacking K+ for 2 d. Then, K+ depletion assays were performed in the presence or absence of vanadate. Further, PMs from K+-starved roots were extracted and used for the kinetic characterization of ATP hydrolytic activity and the immunodetection of PM H+-ATPase. Two major genes coding PM H+-ATPase (SBA1 and SBA2) were analyzed by real-time PCR. PM H+-ATPase exhibited a higher Vmax and Km in NH4+-fed roots compared with NO3--fed roots. The optimum pH of the enzyme was slightly lower in NO3- -fed roots than in NH4+ -fed roots. The vanadate sensitivity was similar. The expressions of SBA1 and SBA2 were increased in roots grown under NH4+. Concomitantly, an increased content of the enzyme in PM was observed. The initial rate of K+ uptake did not differ between plants grown with NO3- or NH4+, but it was significantly reduced by vanadate in NH4+-grown plants.
650 $aAmmonium nitrate
650 $aNitrate fertilizers
650 $aPotassium
650 $aFertilizante nitrogenado
650 $aPotássio
650 $aSorghum Bicolor
650 $aSorgo
653 $aNitrato de Amonia
700 1 $aGOMES FILHO, E.
700 1 $aPRISCO, J. T.
700 1 $aGROSSI DE SA, M. F.
700 1 $aOLIVEIRA NETO, O. B. de
700 1 $aFRAGOSO, R. da R.
773 $tBiologia Plantarum$gv. 58, n. 3, p. 507-514, 2014.
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| Registros recuperados : 102 | |
| 12. |  | PERUSSI FILHO, S.; BISCEGLI, C. I. Criação de novas empresas de base tecnológica via transferência de tecnologias: reflexões sobre o potencial de sucesso do processo de transferência. In: ENCONTRO NACIONAL DE ENGENHARIA DE PRODUÇÃO, 27., 2007, Foz do Iguaçu, PR; [International Conference on Industrial Engineering and Operations Management] - ICIEOM, 13., 2007, Foz do Iguaçu, PR. A energia que move a produção: um diálogo sobre integração, projeto e sustentabilidade. Anais proceedings... [S. L.] : ABEPRO, 2007. p. 1-9. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Instrumentação. |
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| 14. | | BISCEGLI, C. I.; EIRAS, J. A. Development of a portable ultrasound equipment for backfat evaluation of live pigs. In: GAZZINELLI, R.; MOREIRA, R.L.; RODRIGUES, W.N., ed. Physics and industrial development: bridging the gap: proceedings of the 2nd International Conference, Belo Horizonte, MG, July 1996. Singapore: World Scientific, 1997. p. 283-287.| Biblioteca(s): Embrapa Instrumentação. |
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| 16. | | PERUSSI FILHO, S.; BISCEGLI, C. I. Um diagnóstico dos resultados das transferências de tecnologias geradas por uma instituição de pesquisa. In: SEMINÁRIO LATINO-IBEROAMERICANO DE GESTIÓN TECNOLÓGICA, 11., 2005, Salvador. [Anais...]. Salvador: Asociación Latino-Iberoamericana de Gestión Tecnológica. 1 CD-ROM polymers for sensor application. Journal of the Brazilian Chemical Society, São Paulo, v. 16, n. 1, p. 24-30, 2005.| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 102 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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