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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/08/2010 |
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Data da última atualização: |
22/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, E. H.; SOARES, T. L.; SOUZA, F. V. D.; SANTOS-SEREJO, J. A. dos. |
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Afiliação: |
Everton Hilo Souza, UFRB; Tailane Leila Soares, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF. |
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Título: |
Micropropagation of Heliconia rostrata and Heliconia bihai from mature zygotic embryos. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Acta Horticulturae, Leuven, n. 865, p.315-320, 2010. |
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ISSN: |
0567-7572 |
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Idioma: |
Inglês |
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Conteúdo: |
The difficulty in the micropropagation of many species of Heliconaceas has been mainly due to the high frequency of endogenous contamination. Embryo culture techniques have been used to generate normal seedlings to be used as initial explants. This work aimed to develop a plant production protocol using zygotic embryos from Heliconia rostrata and H. bihai. Embryos from mature fruits were disinfested, excised and cultivated in MS medium solidified with 8% of agar, 6% of sucrose (MS + 6% S) and pH 5,8, autoclaved at 120°C for 20 minutes. The treatments were: T01: Control (MS + 6% S); T02: (MS + 6% S) + 0,25% activated charcoal; T03: (MS + 6% S) + 1,0 mg L-1 BAP; T04: (MS + 6% S) + 1,0 mg L-1 BAP + 0,25% activated charcoal; T05: (MS + 6% S) + 1,0 mg L-1 BAP + 1,0 mg L-1 GA3; T06: (MS + 6% S) + 1,0 mg L-1 BAP + 1 mg L-1 GA3 + 0,25% of activated charcoal. The embryos were maintained in dark conditions for 45 days with temperature of 27±1°C. Treatment T02 promoted the best percentage of germination (100%) for both species and T05 the lower results with 20% and 35% of germination for H. rostrata and H. bihai respective at 30 days of culture. Callus formation was observed in T05 and T06. The activated charcoal was essential to embryo germination. These seedlings were used as explants in the micropropagation assays in MS medium supplemented with these treatments: T01: MS; T02: MS + 0,25% of activated charcoal; T03: MS + 4,0 mg L-1 de BAP; T04: MS + 4,0 mg L-1 BAP + 0,25% of activated charcoal. For the multiplication stage the best medium was the control treatment with no growth regulators and no activated charcoal. A bacterial contamination appeared during subsequence subculture making the micropropagation of these species unfeasible. MenosThe difficulty in the micropropagation of many species of Heliconaceas has been mainly due to the high frequency of endogenous contamination. Embryo culture techniques have been used to generate normal seedlings to be used as initial explants. This work aimed to develop a plant production protocol using zygotic embryos from Heliconia rostrata and H. bihai. Embryos from mature fruits were disinfested, excised and cultivated in MS medium solidified with 8% of agar, 6% of sucrose (MS + 6% S) and pH 5,8, autoclaved at 120°C for 20 minutes. The treatments were: T01: Control (MS + 6% S); T02: (MS + 6% S) + 0,25% activated charcoal; T03: (MS + 6% S) + 1,0 mg L-1 BAP; T04: (MS + 6% S) + 1,0 mg L-1 BAP + 0,25% activated charcoal; T05: (MS + 6% S) + 1,0 mg L-1 BAP + 1,0 mg L-1 GA3; T06: (MS + 6% S) + 1,0 mg L-1 BAP + 1 mg L-1 GA3 + 0,25% of activated charcoal. The embryos were maintained in dark conditions for 45 days with temperature of 27±1°C. Treatment T02 promoted the best percentage of germination (100%) for both species and T05 the lower results with 20% and 35% of germination for H. rostrata and H. bihai respective at 30 days of culture. Callus formation was observed in T05 and T06. The activated charcoal was essential to embryo germination. These seedlings were used as explants in the micropropagation assays in MS medium supplemented with these treatments: T01: MS; T02: MS + 0,25% of activated charcoal; T03: MS + 4,0 mg L-1 de BAP; T04: MS + 4,0 mg L-1 BAP + 0,25% of activated... Mostrar Tudo |
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Palavras-Chave: |
Heliconia rostrata; Zygotic embryos. |
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Thesagro: |
Floricultura; Planta Ornamental. |
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Thesaurus Nal: |
Heliconia bihai; micropropagation. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02467naa a2200241 a 4500
001 1859859
005 2022-08-22
008 2010 bl uuuu u00u1 u #d
022 $a0567-7572
100 1 $aSOUZA, E. H.
245 $aMicropropagation of Heliconia rostrata and Heliconia bihai from mature zygotic embryos.$h[electronic resource]
260 $c2010
520 $aThe difficulty in the micropropagation of many species of Heliconaceas has been mainly due to the high frequency of endogenous contamination. Embryo culture techniques have been used to generate normal seedlings to be used as initial explants. This work aimed to develop a plant production protocol using zygotic embryos from Heliconia rostrata and H. bihai. Embryos from mature fruits were disinfested, excised and cultivated in MS medium solidified with 8% of agar, 6% of sucrose (MS + 6% S) and pH 5,8, autoclaved at 120°C for 20 minutes. The treatments were: T01: Control (MS + 6% S); T02: (MS + 6% S) + 0,25% activated charcoal; T03: (MS + 6% S) + 1,0 mg L-1 BAP; T04: (MS + 6% S) + 1,0 mg L-1 BAP + 0,25% activated charcoal; T05: (MS + 6% S) + 1,0 mg L-1 BAP + 1,0 mg L-1 GA3; T06: (MS + 6% S) + 1,0 mg L-1 BAP + 1 mg L-1 GA3 + 0,25% of activated charcoal. The embryos were maintained in dark conditions for 45 days with temperature of 27±1°C. Treatment T02 promoted the best percentage of germination (100%) for both species and T05 the lower results with 20% and 35% of germination for H. rostrata and H. bihai respective at 30 days of culture. Callus formation was observed in T05 and T06. The activated charcoal was essential to embryo germination. These seedlings were used as explants in the micropropagation assays in MS medium supplemented with these treatments: T01: MS; T02: MS + 0,25% of activated charcoal; T03: MS + 4,0 mg L-1 de BAP; T04: MS + 4,0 mg L-1 BAP + 0,25% of activated charcoal. For the multiplication stage the best medium was the control treatment with no growth regulators and no activated charcoal. A bacterial contamination appeared during subsequence subculture making the micropropagation of these species unfeasible.
650 $aHeliconia bihai
650 $amicropropagation
650 $aFloricultura
650 $aPlanta Ornamental
653 $aHeliconia rostrata
653 $aZygotic embryos
700 1 $aSOARES, T. L.
700 1 $aSOUZA, F. V. D.
700 1 $aSANTOS-SEREJO, J. A. dos
773 $tActa Horticulturae, Leuven$gn. 865, p.315-320, 2010.
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