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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
18/09/2015 |
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Data da última atualização: |
24/04/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. G.; CUNHA, E. R.; BLUME, G. R.; MALAQUIAS, J. V.; BÁO, S. N.; MARTINS, C. F. |
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Afiliação: |
JUACI VITORIA MALAQUIAS, CPAC; UNIVERSIDADE DE BRASILIA; CARLOS FREDERICO MARTINS, CPAC. |
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Título: |
Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Cryobiology, v. 70, p. 90-94, 2015. |
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ISSN: |
0011-2240 |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2015.01.001 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cryopreservation technique. MenosAbstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cry... Mostrar Tudo |
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Thesagro: |
Congelamento; Conservação; Criopreservação; Esperma; Inseminação artificial; Suino. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02370naa a2200277 a 4500
001 2024328
005 2025-04-24
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022 $a0011-2240
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2015.01.001$2DOI
100 1 $aSILVA, C. G.
245 $aCryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose.$h[electronic resource]
260 $c2015
520 $aAbstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cryopreservation technique.
650 $aCongelamento
650 $aConservação
650 $aCriopreservação
650 $aEsperma
650 $aInseminação artificial
650 $aSuino
700 1 $aCUNHA, E. R.
700 1 $aBLUME, G. R.
700 1 $aMALAQUIAS, J. V.
700 1 $aBÁO, S. N.
700 1 $aMARTINS, C. F.
773 $tCryobiology$gv. 70, p. 90-94, 2015.
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| Registros recuperados : 7 | |
| 2. |  | HOTT, M. C.; CARVALHO, L. M. T.; SANTOS, P. A.; ARANTES, T. B.; RESENDE, J. C. de. Remote sensing-based estimation of degraded grassland areas in the Zona da Mara region, Minas Gerais state, Brazil. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 51., 2014, Barra dos Coqueiros. A produção animal frente às mudanças climáticas e tecnológicas: resumos. Barra dos coqueiros: Sociedade Brasileira de Zootecnia, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | HOTT, M. C.; CARVALHO, L. M. T. de; SANTOS, P. A. dos; ARANTES, T. B.; RESENDE, J. C. de; FONSECA, L. D'A. M. Análise de perfis temporais de índices de vegetação NDVI em regiões de pastagens na Zona da Mata em Minas Gerais. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | HOTT, M. C.; CARVALHO, L. M. T. de; SANTOS, P. A. dos; ARANTES, T. B.; RESENDE, J. C. de; FONSECA, L. D'A. M. Avaliação espaço-temporal do desenvolvimento de pastagens na Zona da Mata usando dados de sensoriamento remoto. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | HOTT, M. C.; CARVALHO, L. M. T. DE; SANTOS, P. A. D.; ARANTES, T. B.; RESENDE, J. C. de; FONSECA, L. D'A. M.; ANDRADE, R. G. Um cenário de balanço do carbono para Áreas de Preservação Permanentes em mesorregiões leiteiras usando SIG e dados de sensoriamento remoto. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Territorial. |
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| 6. | | RESENDE, J. C. de; HOTT, M. C.; CARVALHO, L. M. T. De; SANTOS, P. A.; ARANTES, T. B.; MAGALHAES JUNIOR, W. C. P. de. Perspectives and scenarios for carbon balance in forest remnants of the Atlantic Forest using remote sensing time series. In: SIMPÓSIO INTERNACIONAL SOBRE GASES DE EFEITO ESTUFA NA AGROPECUÁRIA, 2., 2016. Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | HOTT, M. C.; CARVALHO, L. M. T. de; ANTUNES, M. A. H.; SANTOS, P. A. dos; ARANTES, T. B.; RESENDE, J. C. de; ROCHA, W. S. D. da. Vegetative growth of grasslands based on hyper-temporal NDVI data from the Modis sensor. Pesquisa Agropecuária Brasileira, v. 51, n. 7, p. 858-868, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Unidades Centrais. |
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| Registros recuperados : 7 | |
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