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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/07/2014 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENEZES, S. P.; SILVA, D. M. de A.; LIMA, E. M.; SOUSA, A. O. de; ANDRADE, B. S.; LEMOS, L. S. L.; GRAMACHO, A. P.; GESTEIRA, A. da S.; PIROVANI, C. P.; MICHELI, F. |
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Afiliação: |
SARA PEREIRA MENEZES, UESC; DSON MARIO DE ANDRADE SILVA, UESC; ELINE MATOS LIMA, UESC; AURIZÂNGELA OLIVEIRA DE SOUSA, UESC; BRUNO SILVA ANDRADE; LIVIA SANTOS LIMA LEMOS; ARINA PERES GRAMACHO; ABELMON DA SILVA GESTEIRA, CNPMF; CARLOS PRIMINHO PIROVANI; FABIENNE MICHELI, UESC. |
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Título: |
The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora pernicios. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Plant Biology, 2014. |
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ISSN: |
1471-2229 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively. MenosBackground: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first rep... Mostrar Tudo |
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Thesagro: |
Cacau; Theobroma Cacao. |
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Categoria do assunto: |
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Marc: |
LEADER 02703naa a2200265 a 4500
001 1991551
005 2023-05-25
008 2014 bl uuuu u00u1 u #d
022 $a1471-2229
100 1 $aMENEZES, S. P.
245 $aThe pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora pernicios.$h[electronic resource]
260 $c2014
520 $aBackground: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively.
650 $aCacau
650 $aTheobroma Cacao
700 1 $aSILVA, D. M. de A.
700 1 $aLIMA, E. M.
700 1 $aSOUSA, A. O. de
700 1 $aANDRADE, B. S.
700 1 $aLEMOS, L. S. L.
700 1 $aGRAMACHO, A. P.
700 1 $aGESTEIRA, A. da S.
700 1 $aPIROVANI, C. P.
700 1 $aMICHELI, F.
773 $tBMC Plant Biology, 2014.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 13 | |
| 2. |  | ANDRADE, P. P.; CRUZ, I.; FERREIRA, T. E.; CASTRO, A. L. G. Aspectos biológicos do Díptero Winthemia trinitatis Thompson e viabilidade do parasitismo sobre a lagarta-do-cartucho do milho Spodoptera frugiperda. In: CONGRESSO NACIONAL DE MILHO E SORGO, 27.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 3.; WORKSHOP SOBRE MANEJO E ETIOLOGIA DA MANCHA BRANCA DO MILHO, 2008, Londrina. Agroenergia, produção de alimentos e mudanças climáticas: desafios para milho e sorgo: trabalhos e palestras. [Londrina]: IAPAR; [Sete Lagoas]: Embrapa Milho e Sorgo, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 4. | | FERREIRA, T. E.; CRUZ, I.; CASTRO, A. L. G.; ANDRADE, P. P.; PEREIRA, W. G. Metodologia de criação de ninfas Euborellia annulipes (Lucas) (Dermaptera: carcinophoridae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: resumos... Curitiba: Sociedade Brasileira de Entomologia, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 5. | | SILVA, R. B. S.; MENDES, R. S.; SANTANA, V. L.; SOUZA, H. C.; RAMOS, C. P. S.; SOUZA, A. P.; ANDRADE, P. P.; MELO, M. A. Aspectos epidemiológicos da leishmaniose visceral canina na zona rural do semiárido paraibano e análise de técnicas de diagnóstico. Pesquisa Veterinária Brasileira, Brasília, DF, v.36, n. 7, p. 625-629, jul. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 6. | | WEBER, L. C.; LOPES, A. C. A.; SOUZA, K. R. R. de; ANDRADE, P. P.; GOMES, A. S.; WEBER, R. R.; NASCIMENTO, W. M. Produção e qualidade de sementes híbridas de berinjela em função do número de frutos por planta. Horticultura Brasileira, Brasília, DF, v. 28, n. 2, p. S4315-S4318, jul. 2010. CD-ROM. Suplemento. Trabalho apresentado no Congresso Brasileiro de Olericultura, 50., 2010, Guarapari. Cinquenta anos contribuindo para a saúde da população brasileira. Trabalho A2905-T4454.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 7. | | KIDO, E. A.; PANDOLFI, V.; HOULLOU-KIDO, L. M.; ANDRADE, P. P.; MARCELINO, F. C.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; BURNQUIST, W. L.; BENKO-ISEPPON, A. M. Plant antimicrobial peptides: an overview of superSAGE transcriptional profile and a functional review. Current Protein & Peptide Science, v. 11, n. 3, p. 220-230, May 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | CAVALCANTI, J. J. V.; VASCONCELOS, U.; FARIAS, F. J. C.; SANTOS, R. C. dos; VASCONCELOS, W. S.; ANDRADE, P. P.; ASSUNCAO, J. H. de; ARAUJO, G. P. de; SOUZA, L. da S. S de S. Análise dialélica em algodoeiro (Gossypium hirsutum L.) para tolerância à seca. In: CONGRESSO BRASILEIRO DO ALGODÃO, 10., 2015, Foz do Iguaçu. Resumos. Brasília, DF: ABRAPA, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 10. | | KIDO, E. A.; PANDOLFI, V.; FERREIRA NETO, J. R. C.; BARBOSA, P. D. de A.; ANDRADE, P. P. de; LANE, R.; GUIMARÃES, F. C. M.; ABDELNOOR, R. V.; BENKO-ISEPPON, A. M. Differential transcriptional profiling of phakopsora pachyrhizi-infected soybean revealed by high-thoughput supersage. In: WORLD SOYBEAN RESEARCH CONFERENCE, 9., 2013, Durban. [Proceedings...]. Durban: OPDT: OPOT, 2013. Abst. 387. 1 CD-ROM. WSRC.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | ANDRADE, P. P. de; ARAGAO, F. J. L.; COLLI, W.; DELLAGOSTIN, O. A.; FINARDI-FILHO, F.; HIRATA, M. H.; LIRA-NETO, A. de C.; MELO, M. A. de; NEPOMUCENO, A. L.; NOBREGA, F. G. da; SOUSA, G. D. de; VALICENTE, F. H.; ZANETTINI, M. H. B. Use of transgenic Aedes aegypti in Brazil: risk perception and assessment. Bulletin of the World Health Organization, Geneva, v. 94, p. 766-771, Oct. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | BENKO-ISEPPON, A. M.; KIDO, E. A.; PANDOLFI, V.; BARBOSA, P. K. A; BELARMINO, L. C.; MONTE, S. J. H. do; BRANDÃO, R. M. S. de; ARAUJO, A. de S.; CASTRO, J. A. F. de; SOARES-CAVALCANTI, N. da M.; SILVA, A. R. da; CALSA JUNIOR, T.; ROCHA, M. de M.; WINTER, P.; KAHL, G.; ROTTER, B.; HORRES, R.; MOLINA, C.; JUNGMANN, R.; AMORIM, L. L. B.; ONOFRE, A. V. C.; FERREIRA-NETO, J. R. C.; GRANJEIRO, T. B.; LIMA, A. S.; LOBO, M. D. P.; HOULLOU-KIDO, L. M.; CARVALHO, R. de; WANDERLEY-NOGUEIRA, A. C.; BARROS, P. dos S.; VIEIRA-MELLO, G. S.; BRASILEIRO-VIDAL, A. C.; BORTOLETI, K. C. de A.; PEDROSA-HARAND, A.; ANDRADE, P. P. de; ANDRADE, G. P. de; PIO-RIBEIRO, G.; SITTOLIN, I. M.; FREIRE FILHO, F. R. Brazilian cowpea transcriptome project: over 20 million expressed sequence tags to understand salinity and virus resistance. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010. Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 97-98.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 13. | | KIDO, E. A.; PANDOLFI, V.; BARBOSA, P. K. A.; SILVA, L. C. B. da; SILVA, A. B. da; MONTE, S. J. H. do; BRANDÃO, R. M. S. de S.; ARAUJO, A. de S.; CASTRO, J. A. F. de; SOARES-CAVALCANTI, N. da M.; CALSA-JUNIOR, T.; ROCHA, M. M.; WINTER, P.; KAHL, G.; ROTTER, B.; HORRES, R.; MOLINA, C.; JUNGMANN, R.; AMORIM, L. L. B.; ONOFRE, A. V. C.; FARIAS NETO, J. C.; GRANJEIRO, T. B.; LIMA, A. S.; LOBO, M. D. P.; HOULLOU-KIDO, L. M.; CARVALHO, R. de; WANDERLEY-NOGUEIRA, A. C.; BARROS, P. dos S.; VIEIRA-MELLO, G. S.; BRASILEIRO-VIDAL, A. C.; BORTOLETI, K. C. de A.; PEDROSA-HARAND, A.; ANDRADE, P. P. de; ANDRADE, G. P. de; PIO-RIBEIRO, G.; SITTOLIN, I. M.; FREIRE FILHO, F. R.; CASTRO, L. A. B. de; BENKO-ISEPPON, A. M. Brazilian cowpea transcriptome project: over five million expressed sequence tags to understand salinity and virus resistance. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para a produção de alimentos e bioenergia: livro de resumos. Fortaleza: SBFV: UFC: Embrapa Agroindústria Tropical, 2009. p. 25-26. Resumo 61.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 13 | |
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