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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/01/2019 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHAVES, A. L. A.; CHIAVEGATTO, R. B.; GAVILANES, M. L.; BENITES, F. R. G.; TECHIO, V. H. |
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Afiliação: |
FLAVIO RODRIGO GANDOLFI BENITES, CNPGL. |
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Título: |
Effect of polyploidy on the leaf epidermis structure of Cynodon dactylon (L.) Pers. (Poaceae). |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Biologia, v. 73, n. 10, p. 1007-1013, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Cynodon dactylon has different ploidy levels. The ?gigas effect? in morphoanatomic traits caused by the increase in the genetic material is poorly studied in the species. Thus, the present research aimed at carrying out a quantitative and descriptive study of the leaf epidermis structure in PI291169?04 (2n = 2× = 18); PI477004?26 (2n = 3× = 27); PI291721?22 (2n = 4× = 36); PI289750?10 (2n = 5× = 45) accessions, in order to investigate the consequences of the polyploidy. Adult leaves were fixed in FAA and the paradermal sections obtained through the scraping technique were stained in 5% safranin. The measurements of the stomata characteristics (polar diameters ? PD, equatorial diameters ? QD, stomatal density ? SD, stomatal functionality ? SF, and stomatal index ? SI) were taken by means of the software ImageJ. For analysis in scanning electron microscopy, leaf fragments were fixed in Karnovsky, dehydrated in an acetone series, subjected to a critical point and covered with gold. Stomata were classified as paracytic, dumbbell-shaped and partially covered by papillae. Tector trichomes were classified as uniseriate with pointed tips. In general, as ploidy level increased, there was an increase in stomata dimension and a decrease in SD. Diploid and triploid accessions were easily differentiated in relation to PD and QD, which makes it an indirect method for determining the variation in ploidy level. The variables SD and SI were not efficient to discriminate the accessions. Leaf epidermis has characteristics that emphasize the effect of polyploidization process and its analysis may be used to screening of genotypes in breeding programs. MenosAbstract Cynodon dactylon has different ploidy levels. The ?gigas effect? in morphoanatomic traits caused by the increase in the genetic material is poorly studied in the species. Thus, the present research aimed at carrying out a quantitative and descriptive study of the leaf epidermis structure in PI291169?04 (2n = 2× = 18); PI477004?26 (2n = 3× = 27); PI291721?22 (2n = 4× = 36); PI289750?10 (2n = 5× = 45) accessions, in order to investigate the consequences of the polyploidy. Adult leaves were fixed in FAA and the paradermal sections obtained through the scraping technique were stained in 5% safranin. The measurements of the stomata characteristics (polar diameters ? PD, equatorial diameters ? QD, stomatal density ? SD, stomatal functionality ? SF, and stomatal index ? SI) were taken by means of the software ImageJ. For analysis in scanning electron microscopy, leaf fragments were fixed in Karnovsky, dehydrated in an acetone series, subjected to a critical point and covered with gold. Stomata were classified as paracytic, dumbbell-shaped and partially covered by papillae. Tector trichomes were classified as uniseriate with pointed tips. In general, as ploidy level increased, there was an increase in stomata dimension and a decrease in SD. Diploid and triploid accessions were easily differentiated in relation to PD and QD, which makes it an indirect method for determining the va... Mostrar Tudo |
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Palavras-Chave: |
Gigas effect; Leaf anatomy; Ploidy level. |
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Thesaurus Nal: |
Forage grasses; Stomata. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02429naa a2200229 a 4500
001 2102725
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
100 1 $aCHAVES, A. L. A.
245 $aEffect of polyploidy on the leaf epidermis structure of Cynodon dactylon (L.) Pers. (Poaceae).$h[electronic resource]
260 $c2018
520 $aAbstract Cynodon dactylon has different ploidy levels. The ?gigas effect? in morphoanatomic traits caused by the increase in the genetic material is poorly studied in the species. Thus, the present research aimed at carrying out a quantitative and descriptive study of the leaf epidermis structure in PI291169?04 (2n = 2× = 18); PI477004?26 (2n = 3× = 27); PI291721?22 (2n = 4× = 36); PI289750?10 (2n = 5× = 45) accessions, in order to investigate the consequences of the polyploidy. Adult leaves were fixed in FAA and the paradermal sections obtained through the scraping technique were stained in 5% safranin. The measurements of the stomata characteristics (polar diameters ? PD, equatorial diameters ? QD, stomatal density ? SD, stomatal functionality ? SF, and stomatal index ? SI) were taken by means of the software ImageJ. For analysis in scanning electron microscopy, leaf fragments were fixed in Karnovsky, dehydrated in an acetone series, subjected to a critical point and covered with gold. Stomata were classified as paracytic, dumbbell-shaped and partially covered by papillae. Tector trichomes were classified as uniseriate with pointed tips. In general, as ploidy level increased, there was an increase in stomata dimension and a decrease in SD. Diploid and triploid accessions were easily differentiated in relation to PD and QD, which makes it an indirect method for determining the variation in ploidy level. The variables SD and SI were not efficient to discriminate the accessions. Leaf epidermis has characteristics that emphasize the effect of polyploidization process and its analysis may be used to screening of genotypes in breeding programs.
650 $aForage grasses
650 $aStomata
653 $aGigas effect
653 $aLeaf anatomy
653 $aPloidy level
700 1 $aCHIAVEGATTO, R. B.
700 1 $aGAVILANES, M. L.
700 1 $aBENITES, F. R. G.
700 1 $aTECHIO, V. H.
773 $tBiologia$gv. 73, n. 10, p. 1007-1013, 2018.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 5 | |
| 1. |  | CORREA, J. R. V.; JUNQUEIRA NETTO, A.; REZENDE, P. M. de; ANDRADE, L. A. de B. Efeitos de Rhizobium, Molibdênio e Cobalto sobre o feijoeiro comum cv. Carioca. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 25, n. 4, p. 513-519, abr. 1990.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Unidades Centrais. |
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| 3. | | SANTOS, J. A. C.; ANDRADE, M. A. de; ANDRADE, L. A. de B.; ABREU, A. R. de. Influência de Portes de Cultivares, Números de Capinas e Épocas de Colheita sobre a Incidência de Plantas Daninhas e Produção de Grãos de Milho. Pesquisa Agropecuária Brasileira, Brasília, v.22, n.5, p.501-503, maio 1987.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 4. | | LIMA, P. L. T.; MAGALHAES, C. A. de S.; CARVALHO, R. F. de; LIMA, L. A.; LIMA, J. M. de; ANDRADE, L. A. de B. Trifuralin leaching in soils cultivated with sugarcane irrigated by sub-surface drip system. Irriga, Botucatu, v. 17, n. 1, p. 39-45, janeiro-março, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Roraima. |
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| 5. | | OLIVEIRA, E. L. de; ANDRADE, L. A. de B.; FARIA, M. A. de; EVANGELISTA, A. W. P.; MORAIS, A. R. de. Uso de vinhaça de alambique e nitrogênio em cana-de-açúcar irrigada e não irrigada. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 11, p. 1398-1403, nov. 2009. Título em inglês: Use of pot still vinasse and nitrogen in irrigated and unirrigated sugar cane.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 5 | |
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