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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
23/11/2021 |
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Data da última atualização: |
09/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAIVA, B. A. R. de; WENDLAND, A.; ROSSATO, M.; FERREIRA, M. A. da S. V. |
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Afiliação: |
BRUNA ALICIA RAFAEL DE PAIVA, UNB; ADRIANE WENDLAND FERREIRA, CNPAF; MAURICIO ROSSATO, UNB; MARISA A. DA S. VELLOSO FERREIRA, UNB. |
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Título: |
Virulence and type III effector diversities of Xanthomonas citri pv. fuscans and X. phaseoli pv. phaseoli in Brazil. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Phytopathology, v. 170, n. 1, p. 1-14, 2022. |
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DOI: |
https://doi.org/10.1111/jph.13049 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bacterial blight (CBB) is caused by four genetic lineages belonging to two species of Xanthomonas, namely Xanthomonas citri pv. fuscans (includes fuscans, NF2 and NF3 lineages) and X. phaseoli pv. phaseoli (lineage NF1). A collection of 117 strains of Xanthomonas isolated from common bean plants grown in several producing regions of Brazil, between 2007 and 2016 was established. For species and lineage identification, the following tests were performed: multiplex PCR with a set of four specific primer pairs, pathogenicity tests on susceptible cultivar BRS Artico and phylogenetic analysis based on housekeeping gene sequences. The presence of the two species were confirmed among the 117 strains, being 62 non-fuscans strains (NF1, NF2 and NF3) and 55 fuscans strains of X. citri pv. fuscans. To select a set of representative strains for the virulence assay, a PCR-based analysis of effector diversity was performed with 42 strains belonging to the two species. PCR with primers for xopL, avrBsT, xopE2 and xopE1 genes were positive for all strains, while for the other six effectors there was variation. Six distinct effector profiles were detected, and one strain representing each type was inoculated in 15 common bean cultivars with varying levels of resistance to CBB. The fuscans strains showed uniformity in their effector profiles and were the most virulent. The phylogenetic analyses of our strain collection revealed that all genetic variants of CBB pathogens (NF1, NF2, NF3 and fuscans) are present in Brazil, with significant variability in virulence to common bean cultivars. MenosCommon bacterial blight (CBB) is caused by four genetic lineages belonging to two species of Xanthomonas, namely Xanthomonas citri pv. fuscans (includes fuscans, NF2 and NF3 lineages) and X. phaseoli pv. phaseoli (lineage NF1). A collection of 117 strains of Xanthomonas isolated from common bean plants grown in several producing regions of Brazil, between 2007 and 2016 was established. For species and lineage identification, the following tests were performed: multiplex PCR with a set of four specific primer pairs, pathogenicity tests on susceptible cultivar BRS Artico and phylogenetic analysis based on housekeeping gene sequences. The presence of the two species were confirmed among the 117 strains, being 62 non-fuscans strains (NF1, NF2 and NF3) and 55 fuscans strains of X. citri pv. fuscans. To select a set of representative strains for the virulence assay, a PCR-based analysis of effector diversity was performed with 42 strains belonging to the two species. PCR with primers for xopL, avrBsT, xopE2 and xopE1 genes were positive for all strains, while for the other six effectors there was variation. Six distinct effector profiles were detected, and one strain representing each type was inoculated in 15 common bean cultivars with varying levels of resistance to CBB. The fuscans strains showed uniformity in their effector profiles and were the most virulent. The phylogenetic analyses of our strain collection revealed that all genetic variants of CBB pathogens (NF1, NF2, NF3 ... Mostrar Tudo |
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Palavras-Chave: |
Avirulence genes. |
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Thesagro: |
Doença de Planta; Feijão; Phaseolus Vulgaris; Resistência Genética. |
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Thesaurus Nal: |
Beans; Genetic resistance; Plant diseases and disorders; Xanthomonas axonopodis; Xanthomonas fuscans. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02520naa a2200289 a 4500
001 2136477
005 2022-06-09
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/jph.13049$2DOI
100 1 $aPAIVA, B. A. R. de
245 $aVirulence and type III effector diversities of Xanthomonas citri pv. fuscans and X. phaseoli pv. phaseoli in Brazil.$h[electronic resource]
260 $c2022
520 $aCommon bacterial blight (CBB) is caused by four genetic lineages belonging to two species of Xanthomonas, namely Xanthomonas citri pv. fuscans (includes fuscans, NF2 and NF3 lineages) and X. phaseoli pv. phaseoli (lineage NF1). A collection of 117 strains of Xanthomonas isolated from common bean plants grown in several producing regions of Brazil, between 2007 and 2016 was established. For species and lineage identification, the following tests were performed: multiplex PCR with a set of four specific primer pairs, pathogenicity tests on susceptible cultivar BRS Artico and phylogenetic analysis based on housekeeping gene sequences. The presence of the two species were confirmed among the 117 strains, being 62 non-fuscans strains (NF1, NF2 and NF3) and 55 fuscans strains of X. citri pv. fuscans. To select a set of representative strains for the virulence assay, a PCR-based analysis of effector diversity was performed with 42 strains belonging to the two species. PCR with primers for xopL, avrBsT, xopE2 and xopE1 genes were positive for all strains, while for the other six effectors there was variation. Six distinct effector profiles were detected, and one strain representing each type was inoculated in 15 common bean cultivars with varying levels of resistance to CBB. The fuscans strains showed uniformity in their effector profiles and were the most virulent. The phylogenetic analyses of our strain collection revealed that all genetic variants of CBB pathogens (NF1, NF2, NF3 and fuscans) are present in Brazil, with significant variability in virulence to common bean cultivars.
650 $aBeans
650 $aGenetic resistance
650 $aPlant diseases and disorders
650 $aXanthomonas axonopodis
650 $aXanthomonas fuscans
650 $aDoença de Planta
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aResistência Genética
653 $aAvirulence genes
700 1 $aWENDLAND, A.
700 1 $aROSSATO, M.
700 1 $aFERREIRA, M. A. da S. V.
773 $tJournal of Phytopathology$gv. 170, n. 1, p. 1-14, 2022.
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Embrapa Arroz e Feijão (CNPAF) |
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| 6. |  | ANDRADE, D. S.; HUNGRIA, M. Maximizing the contribution of biological nitrogen fixation in tropical legume crops. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 13., 2001, Ontario. Nitrogen fixation: global perspectives: proceedings. [S.l.]: CABI Publishing, 2002. p. 341-345. Edited by T.M. Finan, M.R. O'Brian, D.B. Layzell, J.K. Vessey, W. Newton.| Biblioteca(s): Embrapa Soja. |
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| 9. | | KASCHUK, G.; HUNGRIA, M.; ANDRADE, D. S. Diversidade de rizóbios que nodulam o feijoeiro (phaseolus vulgaris L.) sob os sistemas de plantios direto e convencional em Ponta grossa, PR. In: WORLD CONGRESS ON CONSERVATION AGRICULTURE = CONGRESSO MUNDIAL SOBRE AGRICULTURA CONSERVACIONISTA, 2., 2003, Foz do Iguaçu. Producing in harmony with nature: [proceedings ...] = Produzindo em harmonia com a natureza: [anais ...]. Ponta Grossa: Federação Brasileira de Plantio Direto na Palha, 2003. v. 2 p. 452-455. II World Congress on Conservation Agriculture, Iguassu Falls, 2003. Extended summary / posters.| Biblioteca(s): Embrapa Soja. |
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