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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
12/11/2013 |
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Data da última atualização: |
21/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JARAMILLO, P. M. D.; GOMES, H. A. R.; SIQUEIRA, F. G. de; MELLO, M. H. de; FILHO, E. X. F.; MAGALHÃES, P. O. |
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Afiliação: |
Paula M. Duque Jaramillo, UnB; Helder A. Rocha Gomes, UnB; FELIX GONCALVES DE SIQUEIRA, CNPAE; Mauricio Homem-de-Mello, UnB; Edivaldo Ximenes Ferreira Filho, UnB; Pérola O. Magalhães, UnB. |
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Título: |
Liquid-liquid extraction of pectinase produced by Aspergillus oryzae using aqueous two-phase micellar system. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Separation and Purification Technology, v. 120, p. 452-457, 2013. |
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DOI: |
10.1016/j.seppur.2013.09.020 |
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Idioma: |
Inglês |
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Conteúdo: |
Separation and purification of proteins, organic compounds and other biomolecules are feasible by liquid?liquid extraction based on phase separation in aqueous micellar solution. This work aimed to opti-mize the extraction of the pectinase enzyme produced by Aspergillus oryzae in a passion fruit peel media, using a micellar Triton X-114/sodium phosphate buffer system. Best condition for this extraction was evaluated in a 2 3 experimental factorial design with a centered face and three repetitions at the central point. The variable analyzed was the pectinase activity, and in this design, it varied from 0.233 to 0.601 U mL1. The highest activity was obtained in the top phase at conditions 25 C, 8% Triton X-114 (wt/wt) and 20% concentrated crude extract (wt/wt). The results indicated that this aqueous two-phase micellar system (ATPMS) was able to remove visible pigments and possible pectinase inhibitors present in fermented broth, that partitioned in the bottom phase. Therefore, this system can be exploited as a first step in pectinase purification from a low cost production system with potential industrial application. |
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Palavras-Chave: |
Aqueous two-phase micellar system; Passion fruit peel; Pectinase; Triton X-114. |
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Thesagro: |
Aspergillus Oryzae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01951naa a2200253 a 4500
001 1971005
005 2017-09-21
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1016/j.seppur.2013.09.020$2DOI
100 1 $aJARAMILLO, P. M. D.
245 $aLiquid-liquid extraction of pectinase produced by Aspergillus oryzae using aqueous two-phase micellar system.$h[electronic resource]
260 $c2013
520 $aSeparation and purification of proteins, organic compounds and other biomolecules are feasible by liquid?liquid extraction based on phase separation in aqueous micellar solution. This work aimed to opti-mize the extraction of the pectinase enzyme produced by Aspergillus oryzae in a passion fruit peel media, using a micellar Triton X-114/sodium phosphate buffer system. Best condition for this extraction was evaluated in a 2 3 experimental factorial design with a centered face and three repetitions at the central point. The variable analyzed was the pectinase activity, and in this design, it varied from 0.233 to 0.601 U mL1. The highest activity was obtained in the top phase at conditions 25 C, 8% Triton X-114 (wt/wt) and 20% concentrated crude extract (wt/wt). The results indicated that this aqueous two-phase micellar system (ATPMS) was able to remove visible pigments and possible pectinase inhibitors present in fermented broth, that partitioned in the bottom phase. Therefore, this system can be exploited as a first step in pectinase purification from a low cost production system with potential industrial application.
650 $aAspergillus Oryzae
653 $aAqueous two-phase micellar system
653 $aPassion fruit peel
653 $aPectinase
653 $aTriton X-114
700 1 $aGOMES, H. A. R.
700 1 $aSIQUEIRA, F. G. de
700 1 $aMELLO, M. H. de
700 1 $aFILHO, E. X. F.
700 1 $aMAGALHÃES, P. O.
773 $tSeparation and Purification Technology$gv. 120, p. 452-457, 2013.
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Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 2 | |
| 1. |  | AMARAL, E. F. do; LIMA, M. V. de O.; LUDEWIGS, T.; ANDRADE, A. do C.; MENESES FILHO, L. C. de L.; RECCO, R. D.; MELO, A. W. F. de; AMARAL, E. F. do. Avaliação do efeito da adubação fosfatada na distribuição do sistema radicular da ingá-de-macaco (Inga coreacea), ingá-mirim (Inga fogifolia) e ingá-de-metro (Inga edulis) cultivadas em aléias no estado do Acre. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 3., 2000, Manaus. Sistemas agroflorestais: manejando a biodiversidade e compondo a paisagem rural: resumos expandidos. Manaus: Embrapa Amazônia Ocidental, 2000. p. 45-47. (Embrapa Amazônia Ocidental. Documentos, 7).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 2. | | AMARAL, E. F. do; LIMA, M. V. de O.; LUDEWIGS, T.; ANDRADE, A. do C.; BARDALES, N. G.; MENESES FILHO, L. C. de L.; RECCO, R. D.; MELO. A. W. F. de; AMARAL, E. F. do. Distribuição dos sistemas radiculares de ingá-de-macaco (Inga coreacea), Ingá-mirim (Inga fagifolia) e Ingá-de-metro (Inga edulis), cultivados em aléias sobre um argissolo amarelo plíntico no estado do Acre. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 3., 2000, Manaus. Sistemas agroflorestais: manejando a biodiversidade e compondo a paisagem rural: resumos expandidos. Manaus: Embrapa Amazônia Ocidental, 2000. p. 144-146. (Embrapa Amazônia Ocidental. Documentos, 7).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| Registros recuperados : 2 | |
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