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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/06/2025 |
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Data da última atualização: |
09/06/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MÜLLER, L. C.; SILVA, P. A. O.; LACORTE, C. C.; SOUSA, M. G. da C.; REZENDE, T. M. B. |
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Afiliação: |
LARISSA CAROLINE MÜLLER, FACULDADE SÃO LEOPOLDO MANDIC; POLIANA AMANDA OLIVEIRA SILVA, FACULDADE SÃO LEOPOLDO MANDIC; CRISTIANO CASTRO LACORTE, CENARGEN; MAURICIO GONÇALVES DA COSTA SOUSA, OREGON HEALTH & SCIENCE UNIVERSITY; TAIA MARIA BERTO REZENDE, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Antibacterial, antibiofilm and viability analysis in vitro of calcium silicate and epoxy resin-based filling sealer. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Brazilia Journal of Oral Sciences, v. 24, e254609, 2025. |
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Idioma: |
Inglês |
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Conteúdo: |
Aim The aim of this study was to evaluate the antibacterial and antibiofilm capacity against Enterococcus faecalis of endodontic sealers AH Plus Jet and the calcium silicate-based sealers, Bio-C Sealer and Sealer Plus BC. Methods All experiments were performed with fresh endodontic sealers without setting. For this, antibacterial agar diffusion test, analysis of antibiofilm activity evaluated by confocal microscopy, assessment of cell viability and pH evaluation were used. Results In the agar diffusion test, all sealers showed an ability to inhibit bacterial growth. In the antibiofilm analysis by confocal microscopy, all the evaluated sealers showed the ability to reduce the mature bacterial biofilm. A pH analysis was also performed in the period from 1min to 72h; calcium silicate-based sealers presented a more alkaline pH than AH Plus Jet, and Bio-C Sealer presented a pH greater than 11 in all the analyzed periods. In the assessment of cell viability AH Plus Jet demonstrated a lower cell viability after 24h. However, after 72h, all sealers led to a reduction in cell viability when compared to control. Conclusions Bio-C Sealer and Sealer Plus BC and AH Plus Jet showed similar antibiofilm capacity against mature E. faecalis biofilms. Furthermore, all evaluated sealers showed antibacterial capacity in the agar diffusion test, and AH Plus Jet and Bio-C Sealer showed significantly greater inhibition of bacterial growth than Sealer Plus BC. Thus, evaluated sealers demonstrated antibacterial and antibiofilm activity against E. faecalis. Knowledge about the antibiofilm and antibacterial activity of endodontic sealers for E. faecalis is relevant, as it is currently one of the main causes of failure in endodontic treatment. MenosAim The aim of this study was to evaluate the antibacterial and antibiofilm capacity against Enterococcus faecalis of endodontic sealers AH Plus Jet and the calcium silicate-based sealers, Bio-C Sealer and Sealer Plus BC. Methods All experiments were performed with fresh endodontic sealers without setting. For this, antibacterial agar diffusion test, analysis of antibiofilm activity evaluated by confocal microscopy, assessment of cell viability and pH evaluation were used. Results In the agar diffusion test, all sealers showed an ability to inhibit bacterial growth. In the antibiofilm analysis by confocal microscopy, all the evaluated sealers showed the ability to reduce the mature bacterial biofilm. A pH analysis was also performed in the period from 1min to 72h; calcium silicate-based sealers presented a more alkaline pH than AH Plus Jet, and Bio-C Sealer presented a pH greater than 11 in all the analyzed periods. In the assessment of cell viability AH Plus Jet demonstrated a lower cell viability after 24h. However, after 72h, all sealers led to a reduction in cell viability when compared to control. Conclusions Bio-C Sealer and Sealer Plus BC and AH Plus Jet showed similar antibiofilm capacity against mature E. faecalis biofilms. Furthermore, all evaluated sealers showed antibacterial capacity in the agar diffusion test, and AH Plus Jet and Bio-C Sealer showed significantly greater inhibition of bacterial growth than Sealer Plus BC. Thus, evaluated sealers demonstrated an... Mostrar Tudo |
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Palavras-Chave: |
Root canal filling materials; Root canal obturation. |
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Thesaurus Nal: |
Endodontics. |
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Categoria do assunto: |
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Marc: |
LEADER 02407naa a2200205 a 4500
001 2176512
005 2025-06-09
008 2025 bl uuuu u00u1 u #d
100 1 $aMÜLLER, L. C.
245 $aAntibacterial, antibiofilm and viability analysis in vitro of calcium silicate and epoxy resin-based filling sealer.$h[electronic resource]
260 $c2025
520 $aAim The aim of this study was to evaluate the antibacterial and antibiofilm capacity against Enterococcus faecalis of endodontic sealers AH Plus Jet and the calcium silicate-based sealers, Bio-C Sealer and Sealer Plus BC. Methods All experiments were performed with fresh endodontic sealers without setting. For this, antibacterial agar diffusion test, analysis of antibiofilm activity evaluated by confocal microscopy, assessment of cell viability and pH evaluation were used. Results In the agar diffusion test, all sealers showed an ability to inhibit bacterial growth. In the antibiofilm analysis by confocal microscopy, all the evaluated sealers showed the ability to reduce the mature bacterial biofilm. A pH analysis was also performed in the period from 1min to 72h; calcium silicate-based sealers presented a more alkaline pH than AH Plus Jet, and Bio-C Sealer presented a pH greater than 11 in all the analyzed periods. In the assessment of cell viability AH Plus Jet demonstrated a lower cell viability after 24h. However, after 72h, all sealers led to a reduction in cell viability when compared to control. Conclusions Bio-C Sealer and Sealer Plus BC and AH Plus Jet showed similar antibiofilm capacity against mature E. faecalis biofilms. Furthermore, all evaluated sealers showed antibacterial capacity in the agar diffusion test, and AH Plus Jet and Bio-C Sealer showed significantly greater inhibition of bacterial growth than Sealer Plus BC. Thus, evaluated sealers demonstrated antibacterial and antibiofilm activity against E. faecalis. Knowledge about the antibiofilm and antibacterial activity of endodontic sealers for E. faecalis is relevant, as it is currently one of the main causes of failure in endodontic treatment.
650 $aEndodontics
653 $aRoot canal filling materials
653 $aRoot canal obturation
700 1 $aSILVA, P. A. O.
700 1 $aLACORTE, C. C.
700 1 $aSOUSA, M. G. da C.
700 1 $aREZENDE, T. M. B.
773 $tBrazilia Journal of Oral Sciences$gv. 24, e254609, 2025.
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| 1. |  | RODRIGUES, L. P. F.; CHAVES, F. C. M.; PADILLA, Y. M.; AMORIM, R. C.; POHLIT, A. M. Germinação e vigor de sementes de caferana (Picrolemma sprucei Hook), família Simaroubaceae. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA OCIDENTAL, 11., 2014, Manaus. Anais... Brasília, DF: Embrapa, 2015. p. 89-90.| Tipo: Resumo em Anais de Congresso |
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| 3. | | POHLIT, A. M.; PINTO, A. C. da S.; CAVALCANTI, B. C.; PESSOA, C. do O.; SILVA, E. C. C. e; CHAVES, F. C. M.; NOGUEIRA, K. L.; LOTUFO, L.; SILVA, L. F. R. e; MELO, M. R. S.; VIEIRA, P. P. R.; MAUSE, R.; AMORIM, R. C. da N.; TADEI, W. P.; ANDRADE-NETO, V. de. Biopharmaceuticals synthesis. In: CARIOCA, J. O. B. (Ed.). Brazilian network on green chemistry - awareness, responsability and action. Benfica: UFC, 2008. p. 347-372.| Tipo: Capítulo em Livro Técnico-Científico |
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| 4. | | ANDRADE-NETO, V. F. de; POHLIT, A. M.; PINTO, A. C. S.; SILVA, E. C. C.; NOGUEIRA, K. L.; MELO, M. R. S.; HENRIQUE, M. C.; AMORIM, R. C. N.; SILVA, L. F. R.; COSTA, M. R. F.; NUNOMURA, R. C. S.; NUNOMURA, S. M.; ALECRIM, W. D.; ALECRIM, M. das G. C.; CHAVES, F. C. M.; VIEIRA, P. P. R. In vitro inhibition of Plasmodium falciparum by substances isolated from Amazonian antimalarial plants. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 3, p. 359-365, jun. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
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| 5. | | LIMA, R. B. S.; SILVA, L. F. R. e; MELO, M. R. S.; COSTA, J. S.; PICANÇO, N. S.; LIMA, E. S.; VASCONCELLOS, M. C.; BOLETI, A. P. A.; SANTOS, J. M. P.; AMORIM, R. C. N.; CHAVES, F. C. M.; COUTINHO, J. P.; TADEI, W. P.; KRETTLI, A. U.; POHLIT, A. M. In vitro and in vivo anti-malarial activity of plants from the Brazilian Amazon. Malaria Journal, v. 14, p. 1-14, Dec. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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