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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
27/09/1996 |
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Data da última atualização: |
15/06/2023 |
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Autoria: |
CALVETE, J. J.; NESSAU, S.; MANN, K.; SANZ, L.; SIEME, H.; KLUG, E.; TOPFER-PETERSEN, E. |
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Título: |
Isolation and biochemical characterization of stallion seminal-plasma proteins. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Reproduction Domestic Animal, v.29, p.411-426, 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Seminal plasma proteins exert multiple effects on sperm function and play a pivotal role in sperm capacitation, a complex process which enables spermatozoa to undergo the zona pellucida-induced acrosome reaction. The role of most of the seminal plasma proteins on sperm physiology is however, not known. This may, in part, reflect the fact that the protein composition of seminal plasma has been investigated in only a few mammalian species. The aim of this study was the characterization of stallion seminal plasma proteins. Partial amino-acid sequences from eight different components were obtained. Among these, a protein with high sequence similarity to rat, mouse, and human epididymal sperm-coating protein, thought to play a role in the fusion of the sperm and egg plasma membranes, a calcitonin precursor gene-related protein, and a prostatic androgen-dependent kallikrein-like product were found. The amino-acid sequences of the other protein fractions failed to show similarity to any known protein structure. Interestingly, as with bovine and pig protein, the major proteins of stallion seminal plasma also constitute the low molecular weight heparin-binding components. However, the major heparin-binding proteins of different species seem to be structurally unrelated to each other, indicating the possibility that heparin-binding proteins could represent species-specific capacitation factors. On the other hand, stallion seminal plasma also contains a minor protein which cross-reacts with antibodies against the boar zona pellucidabinding protein AWN-1. Immunofluorescence analysis shows that the topology of this stallion AWN-like protein is restricted to the equatorial segment. Therefore, the data in this study indicate that proteins participating in sperm-egg interactions are structurally conserved among mammalian species. MenosAbstract: Seminal plasma proteins exert multiple effects on sperm function and play a pivotal role in sperm capacitation, a complex process which enables spermatozoa to undergo the zona pellucida-induced acrosome reaction. The role of most of the seminal plasma proteins on sperm physiology is however, not known. This may, in part, reflect the fact that the protein composition of seminal plasma has been investigated in only a few mammalian species. The aim of this study was the characterization of stallion seminal plasma proteins. Partial amino-acid sequences from eight different components were obtained. Among these, a protein with high sequence similarity to rat, mouse, and human epididymal sperm-coating protein, thought to play a role in the fusion of the sperm and egg plasma membranes, a calcitonin precursor gene-related protein, and a prostatic androgen-dependent kallikrein-like product were found. The amino-acid sequences of the other protein fractions failed to show similarity to any known protein structure. Interestingly, as with bovine and pig protein, the major proteins of stallion seminal plasma also constitute the low molecular weight heparin-binding components. However, the major heparin-binding proteins of different species seem to be structurally unrelated to each other, indicating the possibility that heparin-binding proteins could represent species-specific capacitation factors. On the other hand, stallion seminal plasma also contains a minor protein which cr... Mostrar Tudo |
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Palavras-Chave: |
Plasma seminal; Sperm; Troteina. |
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Thesagro: |
Bioquímica; Garanhão; Proteína. |
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Thesaurus Nal: |
Proteins; Reproduction; Stallions. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
null
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 53 | |
| 4. |  | AMARAL, A. M. do; HOMEM, R. A.; MACHADO, M. A. Expressão de genes de Xanthomonas axonopodis pv. citri através de interação com a planta de citros. Fitopatologia Brasileira, Brasília, DF, v. 32, supl., p. S247, ago. 2007. Resumo 0692. Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, PR, ago. 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | HOMEM, R. A.; BAPTISTA, J. C.; AMARAL, A. M. do; MACHADO, M. A. Análise mutacional de Xanthomonas axonopodis pv. citri para componentes do sistema de secreção tipo III. Summa Phytopathologica, Botucatu, v. 33, supl., p. S14, 2007. Resumo 020. Edição dos Anais do XXX Congresso Paulista de Fitopatologia, 2007, Jaboticabal.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | MACHADO, M. A.; ÁSTUA, J. F.; AMARAL, A. M. do; MALOSSO, A.; MAZZAFERA, P. Gene expression of carotenoid biosynthesis pathway and carotenoid content in different varieties of sweet orange (Citrus sinensis L. Osb.). In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 60. 105.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | AMARAL, A. M. do; DOMINGUES, A. D.; MACHADO, M. A.; CARVALHO, S. A. de. Resposta ao cancro cítrico, em casa-de-vegetação, de plantas de citros de variedades com potencial uso comercial. Fitopatologia Brasileira, Brasília, DF, v. 29, p. S242, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | AMARAL, A. M. do; BAPTISTA, J. C.; SOUZA, C. B. de; RONCOLETTA, J. G. T.; MACHADO, M. A. Adaptação patógeno-hospedeiro e virulência de mutante de Xanthomonas axonopodis pv. citri defectivo para produção de pigmento. Fitopatologia Brasileira, Brasília, DF, v. 29, p. S253, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | CARVALHO, S. A.; CROCE-FLIHO, J.; NUNES, W. M.; AMARAL, A. M. do; SILVA, L. F. C.; MACHADO, M. A. Avaliação da resistência ao cancro cítrico em germoplasma de citros em condições de campo. Fitopatologia Brasileira, v. 30, supl., ago., 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | AMARAL, A. M. do; BAPTISTA, J. C.; BUENO, C. de S.; RONCOLETTA, J. G. T.; MACHADO, M. A. Genome-wide screening for genes functionally related to pathogenicity in Xanthomonas axonopodis pv. citri, the causal agent of Citrus Canker. In: PLANT & ANIMAL GENOME, 13., 2005, San Diego, USA. Abstracts... San Diego: Scherago International, 2005. p. 252| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | GONZALES, S. G.; ASTÚA, J. R.; AMARAL, A. M. do; MARTINS, N. F.; MEHTA, A.; SILVA, M. S.; CARRER, H. Genes associated with hypersensitive response (HR) in the citrus EST database (CitEST). Genetics and Molecular Biology, v. 30, n. 3, suppl., p. 943-956, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 53 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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