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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
23/06/2021 |
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Data da última atualização: |
10/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BUFFO, M. M.; FERREIRA, A. L. Z.; ALMEIDA, R. M. R. G.; FARINAS, C. S.; BADINO, A. C.; XIMENES, E. A.; LADISCH, M. R. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Cellulolytic enzymes production guided by morphology engineering. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Enzyme and Microbial Technology, v. 149, e109833, 2021. |
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Páginas: |
1-8 |
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ISSN: |
0141-0229 |
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DOI: |
https://doi.org/10.1016/j.enzmictec.2021.109833 |
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Idioma: |
Inglês |
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Conteúdo: |
Endoglucanase and xylanase are critical enzymes for liquefaction and enzyme hydrolysis of high solids lignocellulosic biomass to facilitate its transport and production of desired derived products. Here is reported how combinations of different spore concentrations and pH influence microbial morphology, and how this may be used to direct expression and secretion of enzymes by Aspergillus niger. While xylanase production is not affected by A. niger morphology changes, endoglucanase production is enhanced under conditions of lower stress and by morphology that results in pellets. β-glucosidase production is enhanced under dispersed morphology, which results in up to fourfold increase of this enzyme production under the tested experimental conditions. A morphologic scale (Y) is proposed based on a form factor that considers the size and frequency of each morphology class, and that points to conditions that result in high selectivity for either endoglucanase or β-glucosidase production. An equation proposed to relate enzyme activity to morphology provides a useful tool for tuning enzyme production of A. niger, where morphology is a first indication of relative enzyme activities in a fermentation broth. |
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Palavras-Chave: |
(hemi) Cellulases; Filamentous fungi; Morphology engineering. |
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Categoria do assunto: |
-- |
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Marc: |
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001 2132521
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008 2021 bl uuuu u00u1 u #d
022 $a0141-0229
024 7 $ahttps://doi.org/10.1016/j.enzmictec.2021.109833$2DOI
100 1 $aBUFFO, M. M.
245 $aCellulolytic enzymes production guided by morphology engineering.$h[electronic resource]
260 $c2021
300 $a1-8
520 $aEndoglucanase and xylanase are critical enzymes for liquefaction and enzyme hydrolysis of high solids lignocellulosic biomass to facilitate its transport and production of desired derived products. Here is reported how combinations of different spore concentrations and pH influence microbial morphology, and how this may be used to direct expression and secretion of enzymes by Aspergillus niger. While xylanase production is not affected by A. niger morphology changes, endoglucanase production is enhanced under conditions of lower stress and by morphology that results in pellets. β-glucosidase production is enhanced under dispersed morphology, which results in up to fourfold increase of this enzyme production under the tested experimental conditions. A morphologic scale (Y) is proposed based on a form factor that considers the size and frequency of each morphology class, and that points to conditions that result in high selectivity for either endoglucanase or β-glucosidase production. An equation proposed to relate enzyme activity to morphology provides a useful tool for tuning enzyme production of A. niger, where morphology is a first indication of relative enzyme activities in a fermentation broth.
653 $a(hemi) Cellulases
653 $aFilamentous fungi
653 $aMorphology engineering
700 1 $aFERREIRA, A. L. Z.
700 1 $aALMEIDA, R. M. R. G.
700 1 $aFARINAS, C. S.
700 1 $aBADINO, A. C.
700 1 $aXIMENES, E. A.
700 1 $aLADISCH, M. R.
773 $tEnzyme and Microbial Technology$gv. 149, e109833, 2021.
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| Registros recuperados : 5 | |
| 2. |  | SILVA, A. de B.; MULLER, A. A.; SOUZA, L. A. de; ALVES, E. F. Controle biológico da broca do olho do coqueiro e dendezeiro, Rhynchophorus palmarum (L., 1764), em dendezais, no município de Igarapé-Açu, Pará. Belém, PA: Embrapa Amazônia Oriental, 2000. 5 p. il. (Embrapa Amazônia Oriental. Comunicado técnico, 36).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | ESPINDOLA, M. C. B.; COELHO, S. C. S. de S.; SANTOS, D. F. dos; DOURADO, K. C.; ALVES, E. F.; LIMA, R. B.; ALVES, L. A.; ANDRADE, L. L. de; LIMA, M. V. D. de O.; ALTHOFF, T. D.; PEREIRA, G. E. Análises físico-químicas de vinhos experimentais elaborados no Vale do Submédio São Francisco. In: CONGRESSO BRASILEIRO DE VITICULTURA E ENOLOGIA, 12., 2008, Bento Gonçalves. Anais... Bento Gonçalves: Embrapa Uva e Vinho, 2008. p. 162. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 5. | | ESPINDOLA, M. C. B.; COÊLHO, S. C. S. de S.; SANTOS, D. F. dos; DOURADO, K. C.; ALVES, E. F.; LIMA, R. B.; ALVES, L. A.; LIMA, L. L. de A.; LIMA, M. V. D. de O.; ALTHOFF, T. D.; PEREIRA, G. E. Análises físico-químicas de vinhos experimentais elaborados no Vale do Submédio São Francisco. In: CONGRESSO BRASILEIRO DE VITICULTURA E ENOLOGIA, 12., 2008, Bento Gonçalves. Anais... Bento Gonçalves: Embrapa Uva e Vinho, 2008. p. 162.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 5 | |
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