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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
10/01/2010 |
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Data da última atualização: |
22/02/2013 |
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Autoria: |
HOFFMANN, F.; THOMAS, E.; WENZEL, G. |
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Título: |
Anther culture as a breeding tool in rape. II. Progeny analysis of androgenetic lines and induced mutants from haploid cultures. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
Theoretical and Applied Genetics, v. 61, n. 3, p. 225-232, 1982. |
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Idioma: |
Inglês |
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Conteúdo: |
Progeny analysis of androgenetic plants from inbred rape-seed (Brassica napus) shows that selective growth of microspores can occur in cultured anthers. The property of privileged growth in culture seems to be linked to such characters as flowering time and seed glucosinolate content which can be analyzed in regenerated plants. This type of selection and the fact that more variability is visible in regenerants from different microspores than in the progeny of the highly inbred anther donor line, demonstrates the higher degree of homozygosity in the doubled amphihaploids of B. napus. Furthermore, it is shown that haploid genomes of rape may be mutable. Thus it is possible to obtain several different homozygous lines from a single microspore. A system of haploid embryoids arising from single cells of the primary microspore regenerant has also been used to produce experimentally induced mutants. It is demonstrated that recessive mutations can be obtained in a homozygous state in doubled haploid regenerants from mutagenized haploid single cells. |
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Palavras-Chave: |
Cultura de anteras. |
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Thesagro: |
Citogenética; Colza. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01613naa a2200181 a 4500
001 1834074
005 2013-02-22
008 1982 bl --- 0-- u #d
100 1 $aHOFFMANN, F.
245 $aAnther culture as a breeding tool in rape. II. Progeny analysis of androgenetic lines and induced mutants from haploid cultures.
260 $c1982
520 $aProgeny analysis of androgenetic plants from inbred rape-seed (Brassica napus) shows that selective growth of microspores can occur in cultured anthers. The property of privileged growth in culture seems to be linked to such characters as flowering time and seed glucosinolate content which can be analyzed in regenerated plants. This type of selection and the fact that more variability is visible in regenerants from different microspores than in the progeny of the highly inbred anther donor line, demonstrates the higher degree of homozygosity in the doubled amphihaploids of B. napus. Furthermore, it is shown that haploid genomes of rape may be mutable. Thus it is possible to obtain several different homozygous lines from a single microspore. A system of haploid embryoids arising from single cells of the primary microspore regenerant has also been used to produce experimentally induced mutants. It is demonstrated that recessive mutations can be obtained in a homozygous state in doubled haploid regenerants from mutagenized haploid single cells.
650 $aCitogenética
650 $aColza
653 $aCultura de anteras
700 1 $aTHOMAS, E.
700 1 $aWENZEL, G.
773 $tTheoretical and Applied Genetics$gv. 61, n. 3, p. 225-232, 1982.
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