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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
19/02/2019 |
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Data da última atualização: |
19/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLETI, M. D.; REGITANO, L. C. de A.; SOUSA, G. H. M. F.; CESAR, A. S. M.; SIMAS, R. C.; SILVA-VIGNATO, B.; OLIVEIRA, G. B.; ANDRADE, S. C. S.; CAMERON, L. C.; COUTINHO, L. L. |
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Afiliação: |
Mirele D. Poleti, USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; Gustavo H. M. F. Souza, MS Applications and Development Laboratory Waters Corporation; Aline S. M. Cesar, USP; Rosineide C. Simas, USP; Bárbara Silva-Vignato, USP; Gabriella B. Oliveira, USP; Sónia C. S. Andrade, USP; Luiz C. Cameron, UNIRIO; Luiz Lehmann Coutinho, USP. |
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Título: |
Longissimus dorsi muscle label-free quantitative proteomic reveals biological mechanisms associated with intramuscular fat deposition. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 179, p. 30-41, 2018. |
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DOI: |
https://doi.org/10.1016/j.dib.2018.06.004 |
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Idioma: |
Inglês |
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Conteúdo: |
The pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in human health and meat quality attributes, influencing energy metabolism of skeletal muscle, as well as, tenderness, flavor, and juiciness of beef. We performed for the first time the utilization of integrated transcriptome-assisted label-free quantitative proteomic approach using High Definition Mass Spectrometry for characterization of the changes in the proteomic profile of the Longissimus dorsi muscle associated with intramuscular fat deposition in cattle. Furthermore, we compared the muscle proteome with the muscle transcriptome (mRNA and microRNAs), obtained by RNA-sequencing, to better understand the relationship between expression of mRNAs and proteins and to unravel essential biological mechanisms involved in bovine skeletal muscle IMF deposition. MenosThe pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in... Mostrar Tudo |
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Palavras-Chave: |
Adiposidade; Gordura intramuscular; MRNA. |
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Thesagro: |
Gordura Animal. |
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Thesaurus Nal: |
Mass spectrometry; MicroRNA. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03231naa a2200313 a 4500
001 2106242
005 2019-02-19
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.dib.2018.06.004$2DOI
100 1 $aPOLETI, M. D.
245 $aLongissimus dorsi muscle label-free quantitative proteomic reveals biological mechanisms associated with intramuscular fat deposition.$h[electronic resource]
260 $c2018
520 $aThe pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in human health and meat quality attributes, influencing energy metabolism of skeletal muscle, as well as, tenderness, flavor, and juiciness of beef. We performed for the first time the utilization of integrated transcriptome-assisted label-free quantitative proteomic approach using High Definition Mass Spectrometry for characterization of the changes in the proteomic profile of the Longissimus dorsi muscle associated with intramuscular fat deposition in cattle. Furthermore, we compared the muscle proteome with the muscle transcriptome (mRNA and microRNAs), obtained by RNA-sequencing, to better understand the relationship between expression of mRNAs and proteins and to unravel essential biological mechanisms involved in bovine skeletal muscle IMF deposition.
650 $aMass spectrometry
650 $aMicroRNA
650 $aGordura Animal
653 $aAdiposidade
653 $aGordura intramuscular
653 $aMRNA
700 1 $aREGITANO, L. C. de A.
700 1 $aSOUSA, G. H. M. F.
700 1 $aCESAR, A. S. M.
700 1 $aSIMAS, R. C.
700 1 $aSILVA-VIGNATO, B.
700 1 $aOLIVEIRA, G. B.
700 1 $aANDRADE, S. C. S.
700 1 $aCAMERON, L. C.
700 1 $aCOUTINHO, L. L.
773 $tJournal of Proteomics$gv. 179, p. 30-41, 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 31 | |
| 8. |  | PASSOS, L. P.; SUZUKI, L. S.; SILVA, D. P. DA; DIAS, M. L.; ALMEIDA, D. P. DE; MACIEL, T. T. Avaliação de genótipos de cana-de-açúcar visando à resistência ao alumínio e cromo. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília. A produção animal no mundo em transformação: anais. Brasília, DF: SBZ, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | DECARO JUNIOR, S. T.; CORREIA, N. M.; ALMEIDA, D. P.; GOMES, L. de O.; FERREIRA, M. da C. Efeito do uso de adjuvantes na tensão superficial e no ângulo de contato de gotas sobre folhas de ipomoea nil e merremia aegyptia. In: CONGRESSO BRASILEIRO DE FITOSSANIDADE, 3., 2015, Aguas de Lindódia. Anais... São Paulo, SP: UNESP, 2015. 1 CD-ROM. p. 51-54.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 13. | | GONZALES, R. V.; ZAMBOLIM, L.; ALMEIDA, D. P.; CAIXETA, E. T.; ROSADO, R. D. S.; VERDIN-FILHO, A. C. Prospects for achieving multiple disease resistance with Coffea canephora clones. Australasian Plant Pathology, v. 52, n. 5, p. 451-462, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Café. |
|  |
| 14. | | OLIVEIRA, G. de S.; BRAZ, G. B. P.; ALMEIDA, D. P.; PROCOPIO, S. de O.; PAIVA FILHO, S. V. de; SOUZA, M. de F.; SIMON, G. A. Controle de soja voluntária em áreas cultivadas com Crotalaria spectabilis. Revista de Ciências Agroveterinárias, v. 22, n. 3, p. 509-519, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 15. | | SILVA, J. T. da; BARRETO, C. A. V.; NASCIMENTO, A. C. C.; AZEVEDO, C. F.; ALMEIDA, D. P. de; CAIXETA, E. T.; TEIXEIRA, F. R. F.; NASCIMENTO, M. Extreme learning machine for genomic prediction of rust disease resistance in Arabica coffee. Pesquisa Agropecuária Brasileira, v. 61, e03985, 2026. Título em português: Máquina de aprendizado extremo para predição genômica de resistência à ferrugem em café arábica.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Café. |
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| 16. | | ALMEIDA, D. P. de; MOREIRA, K. F.; OLIVEIRA, A. C. B. de; PEREIRA, A. A.; ZAMBOLIM, L.; SAKIYAMA, N. S.; CAIXETA, E. T. Eficácia de marcadores SCAR, CAPS e SSR na seleção assistida de cafeeiros resistentes a Hemileia vastatrix. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 44| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 17. | | MARIZ, B. L.; CAIXETA, E. T.; RESENDE, M. D. V. de; OLIVEIRA, A. C. B. de; ALMEIDA, D. P. de; ALVES, D. R. Exploring the genetic potential for multi-resistance to rust and other coffee phytopathogens in breeding programs. Plants, v. 14, n. 3, 391, 2025. 20 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Café. |
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| 18. | | VIEIRA, I. M.; SILVA, L. M. A.; ALMEIDA, A. G. S. de; ALMEIDA, D. P. de; SILVA-JÚNIOR, O. M.; TAVARES-DIAS, M. Diversity, distribution and new records of freshwater and estuarine shrimp in the state of Amapá, eastern Brazilian Amazon region. Zootaxa, v. 5178, n. 1, p. 041-071, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amapá. |
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| 19. | | SUZUKI, L. S.; PASSOS, L. P.; SANTISTEVAN, G.; REIS, P. R.; CHIAVEGATTO, R. B.; SILVA, C. O.; CAMPOS, F. V. DE; ALMEIDA, D. P. DE. Efeitos do cromo e ácidos orgânicos de baixa massa molecular sobre a nutrição mineral de cana-de-açúcar. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 35., 2012, Águas de Lindóia. Responsabilidade, ética e progresso social: trabalhos. São Paulo: SBQ, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 20. | | FEITOSA, F. de M.; ALMEIDA, D. P. de; ZAMBOLIM, L.; PEREIRA, A. A.; OLIVEIRA, A. C. B. de; CAIXETA, E. T. Seleção assistida por marcadores moleculares de cafeeiros resistentes a ferrugem e antracnose dos frutos. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 98| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 31 | |
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