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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/01/2019 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHAVES, A. L. A.; CHIAVEGATTO, R. B.; GAVILANES, M. L.; BENITES, F. R. G.; TECHIO, V. H. |
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Afiliação: |
FLAVIO RODRIGO GANDOLFI BENITES, CNPGL. |
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Título: |
Effect of polyploidy on the leaf epidermis structure of Cynodon dactylon (L.) Pers. (Poaceae). |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Biologia, v. 73, n. 10, p. 1007-1013, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Cynodon dactylon has different ploidy levels. The ?gigas effect? in morphoanatomic traits caused by the increase in the genetic material is poorly studied in the species. Thus, the present research aimed at carrying out a quantitative and descriptive study of the leaf epidermis structure in PI291169?04 (2n = 2× = 18); PI477004?26 (2n = 3× = 27); PI291721?22 (2n = 4× = 36); PI289750?10 (2n = 5× = 45) accessions, in order to investigate the consequences of the polyploidy. Adult leaves were fixed in FAA and the paradermal sections obtained through the scraping technique were stained in 5% safranin. The measurements of the stomata characteristics (polar diameters ? PD, equatorial diameters ? QD, stomatal density ? SD, stomatal functionality ? SF, and stomatal index ? SI) were taken by means of the software ImageJ. For analysis in scanning electron microscopy, leaf fragments were fixed in Karnovsky, dehydrated in an acetone series, subjected to a critical point and covered with gold. Stomata were classified as paracytic, dumbbell-shaped and partially covered by papillae. Tector trichomes were classified as uniseriate with pointed tips. In general, as ploidy level increased, there was an increase in stomata dimension and a decrease in SD. Diploid and triploid accessions were easily differentiated in relation to PD and QD, which makes it an indirect method for determining the variation in ploidy level. The variables SD and SI were not efficient to discriminate the accessions. Leaf epidermis has characteristics that emphasize the effect of polyploidization process and its analysis may be used to screening of genotypes in breeding programs. MenosAbstract Cynodon dactylon has different ploidy levels. The ?gigas effect? in morphoanatomic traits caused by the increase in the genetic material is poorly studied in the species. Thus, the present research aimed at carrying out a quantitative and descriptive study of the leaf epidermis structure in PI291169?04 (2n = 2× = 18); PI477004?26 (2n = 3× = 27); PI291721?22 (2n = 4× = 36); PI289750?10 (2n = 5× = 45) accessions, in order to investigate the consequences of the polyploidy. Adult leaves were fixed in FAA and the paradermal sections obtained through the scraping technique were stained in 5% safranin. The measurements of the stomata characteristics (polar diameters ? PD, equatorial diameters ? QD, stomatal density ? SD, stomatal functionality ? SF, and stomatal index ? SI) were taken by means of the software ImageJ. For analysis in scanning electron microscopy, leaf fragments were fixed in Karnovsky, dehydrated in an acetone series, subjected to a critical point and covered with gold. Stomata were classified as paracytic, dumbbell-shaped and partially covered by papillae. Tector trichomes were classified as uniseriate with pointed tips. In general, as ploidy level increased, there was an increase in stomata dimension and a decrease in SD. Diploid and triploid accessions were easily differentiated in relation to PD and QD, which makes it an indirect method for determining the va... Mostrar Tudo |
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Palavras-Chave: |
Gigas effect; Leaf anatomy; Ploidy level. |
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Thesaurus Nal: |
Forage grasses; Stomata. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02429naa a2200229 a 4500
001 2102725
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
100 1 $aCHAVES, A. L. A.
245 $aEffect of polyploidy on the leaf epidermis structure of Cynodon dactylon (L.) Pers. (Poaceae).$h[electronic resource]
260 $c2018
520 $aAbstract Cynodon dactylon has different ploidy levels. The ?gigas effect? in morphoanatomic traits caused by the increase in the genetic material is poorly studied in the species. Thus, the present research aimed at carrying out a quantitative and descriptive study of the leaf epidermis structure in PI291169?04 (2n = 2× = 18); PI477004?26 (2n = 3× = 27); PI291721?22 (2n = 4× = 36); PI289750?10 (2n = 5× = 45) accessions, in order to investigate the consequences of the polyploidy. Adult leaves were fixed in FAA and the paradermal sections obtained through the scraping technique were stained in 5% safranin. The measurements of the stomata characteristics (polar diameters ? PD, equatorial diameters ? QD, stomatal density ? SD, stomatal functionality ? SF, and stomatal index ? SI) were taken by means of the software ImageJ. For analysis in scanning electron microscopy, leaf fragments were fixed in Karnovsky, dehydrated in an acetone series, subjected to a critical point and covered with gold. Stomata were classified as paracytic, dumbbell-shaped and partially covered by papillae. Tector trichomes were classified as uniseriate with pointed tips. In general, as ploidy level increased, there was an increase in stomata dimension and a decrease in SD. Diploid and triploid accessions were easily differentiated in relation to PD and QD, which makes it an indirect method for determining the variation in ploidy level. The variables SD and SI were not efficient to discriminate the accessions. Leaf epidermis has characteristics that emphasize the effect of polyploidization process and its analysis may be used to screening of genotypes in breeding programs.
650 $aForage grasses
650 $aStomata
653 $aGigas effect
653 $aLeaf anatomy
653 $aPloidy level
700 1 $aCHIAVEGATTO, R. B.
700 1 $aGAVILANES, M. L.
700 1 $aBENITES, F. R. G.
700 1 $aTECHIO, V. H.
773 $tBiologia$gv. 73, n. 10, p. 1007-1013, 2018.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 6 | |
| 1. |  | VIGNOLO, G. K.; CARPENEDO, S.; SANTOS, I. dos; ALDRIGHI, M.; PICOLOTTO, L.; ANTUNES, L. E. C. Efeitos de épocas de poda e genótipos na qualidade de pêssegos. In: SEMINÁRIO INTERNACIONAL DE EDUCAÇÃO E PESQUISA EM ECOLOGIA, 2., 2009, Pelotas, Resumos...Pelotas: UCPel, 2010. 98-101| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 4. | | VIGNOLO, G. K.; CARPENEDO, S.; ALDRIGHI, M. G.; PEREIRA, I. dos S.; ANTUNES, L. E. C. Comportamento de cultivares de morangueiro na região de Pelotas-RS. In: SIMPÓSIO NACIONAL DO MORANGO, 5.; ENCONTRO SOBRE PEQUENAS FRUTAS E FRUTAS NATIVAS DO MERCOSUL, 4., 2010, Pelotas. Palestras e resumos... Pelotas: Embrapa Clima Temperado, 2010. p. 169-170| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 6. | | FERREIRA, L. V.; COCCO, C.; ALDRIGHI, M.; CARVALHO, S. F. de; PICOLOTTO, L.; MONTE, F.; ANTUNES, L. E. C.; FLORES CANTILLANO, R. F. Efeito da aplicação de cálcio e boro em pré-colheita na qualidade pós-colheita de amoreira-preta Tupy. Revista Iberoamericana de Tecnología Postcosecha, v. 14, n. 1, p. 53-58, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 6 | |
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