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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/08/2002 |
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Data da última atualização: |
07/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOGUERCIO, L. L.; BARRETO, M. L.; ROCHA, T. L.; SANTOS, C. G.; TEIXEIRA, F. F.; PAIVA, E. |
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Afiliação: |
FLAVIA FRANCA TEIXEIRA, CNPMS. |
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Título: |
Combined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Journal of Applied Microbiology, Danvers, v. 93, n. 2, p. 269-277, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolated. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and impact of the study: a simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool forr strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm. |
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Thesagro: |
Genética. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02123naa a2200193 a 4500
001 1485545
005 2018-06-07
008 2002 bl uuuu u00u1 u #d
100 1 $aLOGUERCIO, L. L.
245 $aCombined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm.$h[electronic resource]
260 $c2002
520 $aAims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolated. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and impact of the study: a simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool forr strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm.
650 $aGenética
700 1 $aBARRETO, M. L.
700 1 $aROCHA, T. L.
700 1 $aSANTOS, C. G.
700 1 $aTEIXEIRA, F. F.
700 1 $aPAIVA, E.
773 $tJournal of Applied Microbiology, Danvers$gv. 93, n. 2, p. 269-277, 2002.
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