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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
28/08/2018 |
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Data da última atualização: |
12/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TOSCANO, J. H. B.; LOPES, L. G.; GIRALDELO, L. A.; SILVA, M. H. da; OKINO, C. H.; CHAGAS, A. C. de S. |
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Afiliação: |
João Henrique Barbosa Toscano, UNESP; Louyse Gabrielli Lopes, UNICEP; Luciana Aparecida Giraldelo, UNICEP; Matheus Henrique da Silva, UNICEP; CINTIA HIROMI OKINO, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v.45, n.5, p.1253-1262, out. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Due to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results. MenosDue to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes,... Mostrar Tudo |
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Palavras-Chave: |
Genes de referência. |
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Thesagro: |
Haemonchus Contortus; Ovino. |
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Thesaurus Nal: |
Sheep. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02463naa a2200229 a 4500
001 2094611
005 2018-09-12
008 2018 bl uuuu u00u1 u #d
100 1 $aTOSCANO, J. H. B.
245 $aIdentification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.$h[electronic resource]
260 $c2018
520 $aDue to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results.
650 $aSheep
650 $aHaemonchus Contortus
650 $aOvino
653 $aGenes de referência
700 1 $aLOPES, L. G.
700 1 $aGIRALDELO, L. A.
700 1 $aSILVA, M. H. da
700 1 $aOKINO, C. H.
700 1 $aCHAGAS, A. C. de S.
773 $tMolecular Biology Reports$gv.45, n.5, p.1253-1262, out. 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 16 | |
| 6. |  | FREITAS, J. V. de; OLIVEIRA, Y. M. M. de; ABRANTES, M. A.; MATTOS, P. P. de; SCHAITZA, E. G.; PICHELLI, K. R.; AFONSO, S. R. Memórias do XXV Congresso Mundial da IUFRO, Curitiba, Paraná, Brasil, 29 de setembro a 5 de outubro de 2019. Brasília, DF: Embrapa, 2020. 104 p. il. color. Contém apêndices e anexos.| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Florestas. |
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| 7. | | FREITAS, J. V. de; OLIVEIRA, Y. M. M. de; ABRANTES, M. A.; MATTOS, P. P. de; SCHAITZA, E. G.; PICHELLI, K. R.; AFONSO, S. R. Memories of the 25th IUFRO World Congress, Curitiba, Paraná, Brazil, September 29 to October 5, 2019. Brasília, DF: Embrapa, 2020. 104 p. il. color. Contém apêndices e anexos.| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Florestas. |
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| 8. | | SOUZA, L. S.; MOREIRA, R. F. C.; FREITAS, T. A. S. de; MENDONÇA, A. V. R.; AFONSO, S. D. J.; LEDO, C. A. da S. Grouping of catingueira genotypes based on morphological characteristics. Revista Árvore, Viçosa-MG, v.40, n.3, p.427-434, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | AFONSO, S. D. J.; MOREIRA, R. F. C.; LEDO, C. A. da S.; FERREIRA, C. F.; SANTOS, V. da S.; MUONDO, P. A. Genetic structure of cassava populations (Manihot esculenta Crantz) from Angola assessed through (ISSR) markers. African Journal of Biotechnology, v. 18, n.7, p. 144-154, February, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | AFONSO, S. D. J.; LEDO, C. A. da S.; MOREIRA, R. F. C.; SANTOS, V. da S.; BORGES, V. P.; MUONDO, P. A. Selection of cassava (Manihot esculenta Crantz) genotypes based on agro-morphological traits in Angola. African Journal of Biotechnology, v. 18, n.7, p. 144-154, February, 2019.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | AFONSO, S. D. J.; LEDO, C. A. da S.; MOREIRA, R. F. C.; SILVA, S. de O. e; LEAL, V. D. de J.; CONCEIÇÃO, A. L. da S. Selection of descriptors in a morphological characteristics considered in cassava accessions by means of multivariate techniques. Journal of Agriculture and Veterinary Science, v. 7, Issue 1, p 13-20, Feb. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | MOREIRA, R. F, C.; AFONSO, S. D. J.; LEDO, C. A. da S.; SILVA, S. A.; CONCEIÇÃO, A. L. da S.; CERQUEIRA-PEREIRA, E. C.; LINGE, C. da S. Agro-morphological diversity in yam genotypes from Recôncavo of Bahia, Brazil. African Journal of Agricultural, v. 12, n.24, p. 2070-2077, June, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | MATTOS, L. M. de; AFONSO, S. R.; BRITO LIMA, M. de F. de; ALMEIDA, R. de; CHILES, J. M.; FAVARO, S. P.; MOREIRA, A. C. de O.; MALAQUIAS, J. V.; SEVILHA, A. C. Caracterização e análise financeira do processo de extração de óleo de polpa de pequi no Cerrado brasileiro. Planaltina, DF: Embrapa Cerrados, 2021. 64 p. (Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 377).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | MATTOS, L. M. de; AFONSO, S. R.; BRITO LIMA, M. de F. de; ALMEIDA, R. de; CHILES, J. M.; FAVARO, S. P.; MOREIRA, A. C. de O.; MALAQUIAS, J. V.; SEVILHA, A. C. Caracterização e análise financeira do processo de extração de óleo de polpa de pequi no Cerrado brasileiro. Planaltina, DF: Embrapa Cerrados, 2021. 64 p. (Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 377).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Cerrados. |
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| 16. | | GINJA, C.; GAMA, L. T.; CORTÉS, O.; BURRIEL, I. M.; VEGA-PLA , J. L.; PENEDO, C.; SPONENBERG, P.; CAÑÓN, J.; SANZ, A.; EGITO, A. A. do; ALVAREZ, L. A.; GIOVAMBATTISTA, G.; AGHA, S.; ROGBERG-MUÑOZ, A.; LARA, M. A. C.; DELGADO, J. V.; MARTINEZ, A.; AFONSO, S.; AGUIRRE, L.; ARMSTRONG, E.; VALLEJO, M. E. C.; CANALES, A.; CASSAMÁ, B.; CONTRERAS, G; CORDEIRO, J. M. M.; DUNNER, S.; ELBELTAGY, A.; FIORAVANTI, M. C. S.; CARPIO, M. G.; GÓMEZ, M.; HERNÁNDEZ, A.; HERNANDEZ, D.; JULIANO, R. S.; LANDI, V.; MARQUES, J. R.; MARTÍNEZ, R. D.; MARTÍNEZ, O. R.; MELUCCI, L.; FLORES, B. M.; MÚJICA, F.; PARÉS I CASANOVA, P. M.; QUIROZ, J.; RODELLAR, C.; TJON, G.; ADEBAMBO, T.; UFFO, O.; VARGAS, J. C.; VILLALOBOS, A.; ZARAGOZA, P. The genetic ancestry of american creole cattle inferred from uniparental and autosomal genetic markers. Scientific Reports, v. 9, n. 11486, p. 1-16, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 16 | |
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