| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
18/12/2019 |
|
Data da última atualização: |
06/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ARAÚJO, R. S.; GARCIA, G. M.; VILELA, J. M. C.; ANDRADE, M. S.; OLIVEIRA, L. A. M.; KANO, E. K.; LANGE, C. C.; BRITO, M. A. V. P. E; BRANDAO, H. de M.; MOSQUEIRA, V. C. F. |
|
Afiliação: |
RAQUEL SILVA ARAÚJO; GIANI MARTINS GARCIA; JOSÉ MARIO CARNEIRO VILELA; MARGARETH SPANGLER ANDRADE; LASER ANTÔNIO MACHADO OLIVEIRA; EUNICE KAZUE KANO; CARLA CHRISTINE LANGE, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; HUMBERTO DE MELLO BRANDAO, CNPGL; VANESSA CARLA FURTADO MOSQUEIRA. |
|
Título: |
Cloxacillin benzathine-loaded polymeric nanocapsules: Physicochemical characterization, cell uptake, and intramammary antimicrobial effect. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Materials Science & Engineering C, v. 104, 110006, 2019. |
|
DOI: |
https://doi.org/10.1016/j.msec.2019.110006 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tration of CLOXB-PCL NC in cows with mastitis resulted in no clinical signs of toxicity and allowed completepathogen elimination after treatment. Thein vivoresults obtained in this work suggest that CLOXB-PCL NC couldbe a promising formulation for the treatment of intramammary infections in cattle, considering their physico-chemical properties, release profiles and effects on bovine mastitis control. MenosThe present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tra... Mostrar Tudo |
|
Palavras-Chave: |
Cell uptake; Cloxacillin benzathine; Intramammary; Release kinetics. |
|
Thesaurus Nal: |
Mastitis; Nanocapsules. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02882naa a2200313 a 4500
001 2117206
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.msec.2019.110006$2DOI
100 1 $aARAÚJO, R. S.
245 $aCloxacillin benzathine-loaded polymeric nanocapsules$bPhysicochemical characterization, cell uptake, and intramammary antimicrobial effect.$h[electronic resource]
260 $c2019
520 $aThe present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tration of CLOXB-PCL NC in cows with mastitis resulted in no clinical signs of toxicity and allowed completepathogen elimination after treatment. Thein vivoresults obtained in this work suggest that CLOXB-PCL NC couldbe a promising formulation for the treatment of intramammary infections in cattle, considering their physico-chemical properties, release profiles and effects on bovine mastitis control.
650 $aMastitis
650 $aNanocapsules
653 $aCell uptake
653 $aCloxacillin benzathine
653 $aIntramammary
653 $aRelease kinetics
700 1 $aGARCIA, G. M.
700 1 $aVILELA, J. M. C.
700 1 $aANDRADE, M. S.
700 1 $aOLIVEIRA, L. A. M.
700 1 $aKANO, E. K.
700 1 $aLANGE, C. C.
700 1 $aBRITO, M. A. V. P. E
700 1 $aBRANDAO, H. de M.
700 1 $aMOSQUEIRA, V. C. F.
773 $tMaterials Science & Engineering C$gv. 104, 110006, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Florestas. |
|
Data corrente: |
30/09/2021 |
|
Data da última atualização: |
06/12/2021 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
CARVALHO, M. T. de M.; FAUSTINO, L. L.; ARAÚJO, W. A. de; PACHECO, A. R.; TROGELLO, E.; OLIVEIRA, S. M. de; MACHADO, P. L. O. de A.; SIQUEIRA, M. M. de B.; SILVA, R. R. da; LIMA, J. A. |
|
Afiliação: |
MARCIA THAIS DE MELO CARVALHO, CNPAF; LUCAS LUÍS FAUSTINO; WILKER ALVES DE ARAUJO; ABILIO RODRIGUES PACHECO, CNPF; EMERSON TROGELLO, INSTITUTO FEDERAL GOIANO; SERGIO MARTINS DE OLIVEIRA, EMATER-GO; PEDRO LUIZ OLIVEIRA DE A MACHADO, CNPAF; MATHEUS MENTONE DE BRITTO SIQUEIRA; RYAN RODRIGUES DA SILVA; JULIANA ALVES LIMA. |
|
Título: |
Trees improve soil carbon stocks in integrated crop-livestock-forestry (ICLF) systems. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS: 100% DIGITAL, 2., 2021. WCCLF 2021 proceedings. Campo Grande, MS: Embrapa Gado de Corte, 2021. |
|
Páginas: |
p. 134-139. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Soil organic carbon (SOC, determined with dichromate oxidation) accumulation was evaluated at three ICLF sites of different ages (2, 4 and 11 years) and soil textures (469, 198, 632 g kg-1 clay) in southern Goiás state of Brazil. SOC stocks were accessed at 0-30, 30-100 and 0-100 cm soil layers under the tree-rows and between tree-rows. SOC stocks were calculated based on equivalent soil mass, using pasture, in which the ICLFs were implemented, as reference. Our objective was to investigate the potential of trees in accumulating SOC in ICLFs. Dunnett?s test was applied to check the effect of trees on soil C stocks within each location using the linear mixed model. More SOC was measured under tree-rows at all sites and all soil layers, compared to the alley between the tree-rows, except for the youngest site (Morrinhos) at 0-30 cm. Trees, therefore, are important to maximize SOC accumulation in ICLFs. Further studies are, however, necessary to expand the database to support more robust analysis and to conclude on SOC sequestration (immobilization). |
|
Palavras-Chave: |
Integração lavoura-pecuária-floresta; Neotropical savanna. |
|
Thesagro: |
Carbono; Cerrado. |
|
Thesaurus NAL: |
Savanna soils; Soil organic carbon. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
Marc: |
LEADER 02098naa a2200313 a 4500
001 2134926
005 2021-12-06
008 2021 bl uuuu u00u1 u #d
100 1 $aCARVALHO, M. T. de M.
245 $aTrees improve soil carbon stocks in integrated crop-livestock-forestry (ICLF) systems.$h[electronic resource]
260 $c2021
300 $ap. 134-139.
520 $aSoil organic carbon (SOC, determined with dichromate oxidation) accumulation was evaluated at three ICLF sites of different ages (2, 4 and 11 years) and soil textures (469, 198, 632 g kg-1 clay) in southern Goiás state of Brazil. SOC stocks were accessed at 0-30, 30-100 and 0-100 cm soil layers under the tree-rows and between tree-rows. SOC stocks were calculated based on equivalent soil mass, using pasture, in which the ICLFs were implemented, as reference. Our objective was to investigate the potential of trees in accumulating SOC in ICLFs. Dunnett?s test was applied to check the effect of trees on soil C stocks within each location using the linear mixed model. More SOC was measured under tree-rows at all sites and all soil layers, compared to the alley between the tree-rows, except for the youngest site (Morrinhos) at 0-30 cm. Trees, therefore, are important to maximize SOC accumulation in ICLFs. Further studies are, however, necessary to expand the database to support more robust analysis and to conclude on SOC sequestration (immobilization).
650 $aSavanna soils
650 $aSoil organic carbon
650 $aCarbono
650 $aCerrado
653 $aIntegração lavoura-pecuária-floresta
653 $aNeotropical savanna
700 1 $aFAUSTINO, L. L.
700 1 $aARAÚJO, W. A. de
700 1 $aPACHECO, A. R.
700 1 $aTROGELLO, E.
700 1 $aOLIVEIRA, S. M. de
700 1 $aMACHADO, P. L. O. de A.
700 1 $aSIQUEIRA, M. M. de B.
700 1 $aSILVA, R. R. da
700 1 $aLIMA, J. A.
773 $tIn: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS: 100% DIGITAL, 2., 2021. WCCLF 2021 proceedings. Campo Grande, MS: Embrapa Gado de Corte, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Florestas (CNPF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
