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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
08/01/1997 |
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Data da última atualização: |
28/04/2023 |
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Autoria: |
QUINN, P. J.; WHITE, I. G.; WIRRICK, B. R. |
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Título: |
The effect of dilution on the concentration of sodium, potassium, calcium and magnesium in ram and bull spermatozoa. |
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Ano de publicação: |
1966 |
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Fonte/Imprenta: |
Journal Reproduction and Fertility, v. 12, n. 1, p. 131-138, Aug. 1966. |
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DOI: |
10.1530/jrf.0.0120131 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Mild dilution (<1·8) of ram and bull semen in isotonic sodium phosphate diluent of pH 7·0 caused an influx of sodium and an efflux of potassium, calcium and magnesium from the spermatozoa. Equilibrium was reached soon after dilution and little further change in the concentration of these cations was observed over 3 hr. Similar changes occurred when ram and bull semen was diluted in tris and veronal diluents of pH 7; however, calcium was found to accumulate in ram spermatozoa diluted in tris. Diluting semen in buffers of pH range 8·5 to 5·5 indicated that potassium loss from the spermatozoa increased with increasing pH. Greater dilution (1:100) and exhaustive washing (four times) had a similar but more severe effect on the cation concentration of ram and bull spermatozoa. A large influx of sodium was accompanied by a marked depletion of potassium which tended to be further depleted over 3 hr. |
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Palavras-Chave: |
Bioquimica seminal. |
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Thesagro: |
Cálcio; Magnésio; Potássio; Reprodução; Sódio. |
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Thesaurus Nal: |
Bulls; Cations; Cattle; Rams; Sheep. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01731naa a2200289 a 4500
001 1522918
005 2023-04-28
008 1966 bl uuuu u00u1 u #d
024 7 $a10.1530/jrf.0.0120131$2DOI
100 1 $aQUINN, P. J.
245 $aThe effect of dilution on the concentration of sodium, potassium, calcium and magnesium in ram and bull spermatozoa.$h[electronic resource]
260 $c1966
520 $aAbstract: Mild dilution (<1·8) of ram and bull semen in isotonic sodium phosphate diluent of pH 7·0 caused an influx of sodium and an efflux of potassium, calcium and magnesium from the spermatozoa. Equilibrium was reached soon after dilution and little further change in the concentration of these cations was observed over 3 hr. Similar changes occurred when ram and bull semen was diluted in tris and veronal diluents of pH 7; however, calcium was found to accumulate in ram spermatozoa diluted in tris. Diluting semen in buffers of pH range 8·5 to 5·5 indicated that potassium loss from the spermatozoa increased with increasing pH. Greater dilution (1:100) and exhaustive washing (four times) had a similar but more severe effect on the cation concentration of ram and bull spermatozoa. A large influx of sodium was accompanied by a marked depletion of potassium which tended to be further depleted over 3 hr.
650 $aBulls
650 $aCations
650 $aCattle
650 $aRams
650 $aSheep
650 $aCálcio
650 $aMagnésio
650 $aPotássio
650 $aReprodução
650 $aSódio
653 $aBioquimica seminal
700 1 $aWHITE, I. G.
700 1 $aWIRRICK, B. R.
773 $tJournal Reproduction and Fertility$gv. 12, n. 1, p. 131-138, Aug. 1966.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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