Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
29/08/2002 |
Data da última atualização: |
07/06/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LOGUERCIO, L. L.; BARRETO, M. L.; ROCHA, T. L.; SANTOS, C. G.; TEIXEIRA, F. F.; PAIVA, E. |
Afiliação: |
FLAVIA FRANCA TEIXEIRA, CNPMS. |
Título: |
Combined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Journal of Applied Microbiology, Danvers, v. 93, n. 2, p. 269-277, 2002. |
Idioma: |
Inglês |
Conteúdo: |
Aims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolated. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and impact of the study: a simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool forr strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm. |
Thesagro: |
Genética. |
Categoria do assunto: |
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Marc: |
LEADER 02123naa a2200193 a 4500 001 1485545 005 2018-06-07 008 2002 bl uuuu u00u1 u #d 100 1 $aLOGUERCIO, L. L. 245 $aCombined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm.$h[electronic resource] 260 $c2002 520 $aAims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolated. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and impact of the study: a simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool forr strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm. 650 $aGenética 700 1 $aBARRETO, M. L. 700 1 $aROCHA, T. L. 700 1 $aSANTOS, C. G. 700 1 $aTEIXEIRA, F. F. 700 1 $aPAIVA, E. 773 $tJournal of Applied Microbiology, Danvers$gv. 93, n. 2, p. 269-277, 2002.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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