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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
07/03/2017 |
Data da última atualização: |
07/03/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
WEIS, G. M.; ABOT, A. R.; TORRES, F. Z. V.; VALERIO, J. R.; ALLEGRETTI NETO, A. R.; LIRA, E. da C.; OLIVEIRA, M. C. M. |
Afiliação: |
GLENDA M. WEIS, UEMS; ALFREDO R. ABOT, UEMS; FABRICIA ZIMERMANN VILELA TORRES, CNPGC; JOSE RAUL VALERIO, CNPGC; ALBERTO R. ALLEGRETTI NETO, UEMS; EWERTON DA C. LIRA, Bolsista CNPq; MARLENE C. M. OLIVEIRA, AGRAER/MS. |
Título: |
Flutuação populacional de Scaptocoris carvalhoi (Beker, 1967) em área irrigada de Panicum maximum cv. Monbaça na região de ecótono do Cerrado-Pantanal. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 26; CONGRESSO LATINO-AMERICANO DE ENTOMOGIA, 9., 2016. Maceió. Anais... Brasília, DF: Embrapa 2016. 670 p. |
Páginas: |
p. 186 |
Idioma: |
Português |
Palavras-Chave: |
Bioecologia; Praga de solo. |
Thesagro: |
Percevejo castanho. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157228/1/Flutuacao-populacional-de-Scaptocoris.pdf
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Marc: |
LEADER 00828nam a2200217 a 4500 001 2066400 005 2017-03-07 008 2016 bl uuuu u00u1 u #d 100 1 $aWEIS, G. M. 245 $aFlutuação populacional de Scaptocoris carvalhoi (Beker, 1967) em área irrigada de Panicum maximum cv. Monbaça na região de ecótono do Cerrado-Pantanal.$h[electronic resource] 260 $aCONGRESSO BRASILEIRO DE ENTOMOLOGIA, 26; CONGRESSO LATINO-AMERICANO DE ENTOMOGIA, 9., 2016. Maceió. Anais... Brasília, DF: Embrapa 2016. 670 p.$c2016 300 $ap. 186 650 $aPercevejo castanho 653 $aBioecologia 653 $aPraga de solo 700 1 $aABOT, A. R. 700 1 $aTORRES, F. Z. V. 700 1 $aVALERIO, J. R. 700 1 $aALLEGRETTI NETO, A. R. 700 1 $aLIRA, E. da C. 700 1 $aOLIVEIRA, M. C. M.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
Data corrente: |
17/03/2014 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. |
Afiliação: |
MARCIO ROBERTO SILVA, CNPGL. |
Título: |
Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Plos One, v. 9, n. 3, p. 1-6, 2014 |
Idioma: |
Inglês Português |
Conteúdo: |
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. MenosIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with ... Mostrar Tudo |
Palavras-Chave: |
Bovine and bubaline tuberculosis; Nested-PCR system. |
Thesaurus NAL: |
Mycobacterium abscessus. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119848/1/ARAUJO-C.-P..pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99300/1/pone.0091023-1..6-2014-Nested-PCR.pdf
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Marc: |
LEADER 02739naa a2200325 a 4500 001 2010358 005 2024-02-05 008 2014 bl uuuu u00u1 u #d 100 1 $aARAUJO, C. P. 245 $aDetection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1.$h[electronic resource] 260 $c2014 520 $aIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. 650 $aMycobacterium abscessus 653 $aBovine and bubaline tuberculosis 653 $aNested-PCR system 700 1 $aOSÓRIO, A. L. A. R. 700 1 $aJORGE, K. S. G. 700 1 $aRAMOS, C. A. N. 700 1 $aS. FILHO, A. F. 700 1 $aVIDAL, C. E. S. 700 1 $aROXO, E. 700 1 $aNISHIBE, c. 700 1 $aALMEIDA, N. F. 700 1 $aF. JUNIOR, A. A. 700 1 $aSILVA, M. R. 700 1 $aB. NETO, J. D. 700 1 $aCERQUEIRA, V. D. 700 1 $aZUMÁRRAGA, M. 700 1 $aARAUJO, F. R. 773 $tPlos One$gv. 9, n. 3, p. 1-6, 2014
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