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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
29/11/2022 |
Data da última atualização: |
29/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FREGULIA, P.; CAMPOS, M. M.; DIAS, R. J. P.; LIU, J.; GUO, W.; PEREIRA, L. G. R.; MACHADO, M. A.; REIS, D. R. de L.; GUAN, L. L.; GARNSWORTHY, P. C.; NEVES, A. L. A. |
Afiliação: |
PRISCILA FREGULIA, Universidade Federal de Juiz de Fora, Juiz de Fora; MARIANA MAGALHAES CAMPOS, CNPGL; ROBERTO JÚNIO PEDROSO DIAS, Universidade Federal de Juiz de Fora; JUNHONG LIU, University of Alberta; WEI GUO, Guizhou University; LUIZ GUSTAVO RIBEIRO PEREIRA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; LE LUO GUAN, University of Alberta; PHIL C. GARNSWORTHY, University of Nottingham; ANDRÉ LUIS ALVES NEVES, University of Copenhagen. |
Título: |
Taxonomic and predicted functional signatures reveal linkages between the rumen microbiota and feed efficiency in dairy cattle raised in tropical areas. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Frontiers in Microbiology, v. 13, 1025173, 2022. |
DOI: |
https://doi.org/10.3389/fmicb.2022.1025173 |
Idioma: |
Inglês |
Conteúdo: |
Ruminants digest plant biomass more efficiently than monogastric animals due to their symbiotic relationship with a complex microbiota residing in the rumen environment. What remains unclear is the relationship between the rumen microbial taxonomic and functional composition and feed efficiency (FE), especially in crossbred dairy cattle (Holstein x Gyr) raised under tropical conditions. In this study, we selected twenty-two F1 Holstein x Gyr heifers and grouped them according to their residual feed intake (RFI) ranking, high efficiency (HE) (n = 11) and low efficiency (LE) (n = 11), to investigate the effect of FE on the rumen microbial taxa and their functions. Rumen fluids were collected using a stomach tube apparatus and analyzed using amplicon sequencing targeting the 16S (bacteria and archaea) and 18S (protozoa) rRNA genes. Alpha-diversity and beta-diversity analysis revealed no significant difference in the rumen microbiota between the HE and LE animals. Multivariate analysis (sPLS-DA) showed a clear separation of two clusters in bacterial taxonomic profiles related to each FE group, but in archaeal and protozoal profiles, the clusters overlapped. The sPLS-DA also revealed a clear separation in functional profiles for bacteria, archaea, and protozoa between the HE and LE animals. Microbial taxa were differently related to HE (e.g., Howardella and Shuttleworthia) and LE animals (e.g., Eremoplastron and Methanobrevibacter), and predicted functions were significatively different for each FE group (e.g., K03395?signaling and cellular process was strongly related to HE animals, and K13643?genetic information processing was related to LE animals). This study demonstrates that differences in the rumen microbiome relative to FE ranking are not directly observed from diversity indices (Faith?s Phylogenetic Diversity, Pielou?s Evenness, Shannon?s diversity, weighted UniFrac distance, Jaccard index, and Bray?Curtis dissimilarity), but from targeted identification of specific taxa and microbial functions characterizing each FE group. These results shed light on the role of rumen microbial taxonomic and functional profiles in crossbred Holstein × Gyr dairy cattle raised in tropical conditions, creating the possibility of using the microbial signature of the HE group as a biological tool for the development of biomarkers that improve FE in ruminants. MenosRuminants digest plant biomass more efficiently than monogastric animals due to their symbiotic relationship with a complex microbiota residing in the rumen environment. What remains unclear is the relationship between the rumen microbial taxonomic and functional composition and feed efficiency (FE), especially in crossbred dairy cattle (Holstein x Gyr) raised under tropical conditions. In this study, we selected twenty-two F1 Holstein x Gyr heifers and grouped them according to their residual feed intake (RFI) ranking, high efficiency (HE) (n = 11) and low efficiency (LE) (n = 11), to investigate the effect of FE on the rumen microbial taxa and their functions. Rumen fluids were collected using a stomach tube apparatus and analyzed using amplicon sequencing targeting the 16S (bacteria and archaea) and 18S (protozoa) rRNA genes. Alpha-diversity and beta-diversity analysis revealed no significant difference in the rumen microbiota between the HE and LE animals. Multivariate analysis (sPLS-DA) showed a clear separation of two clusters in bacterial taxonomic profiles related to each FE group, but in archaeal and protozoal profiles, the clusters overlapped. The sPLS-DA also revealed a clear separation in functional profiles for bacteria, archaea, and protozoa between the HE and LE animals. Microbial taxa were differently related to HE (e.g., Howardella and Shuttleworthia) and LE animals (e.g., Eremoplastron and Methanobrevibacter), and predicted functions were significatively di... Mostrar Tudo |
Palavras-Chave: |
Composição microbiana; Eficiência alimentar; Microbiota. |
Thesagro: |
Bovino; Gado Leiteiro. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148891/1/Taxonomic-and-predicted-functional-signatures-reveal-linkages-between-the-rumen.pdf
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Marc: |
LEADER 03356naa a2200313 a 4500 001 2148891 005 2022-11-29 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3389/fmicb.2022.1025173$2DOI 100 1 $aFREGULIA, P. 245 $aTaxonomic and predicted functional signatures reveal linkages between the rumen microbiota and feed efficiency in dairy cattle raised in tropical areas.$h[electronic resource] 260 $c2022 520 $aRuminants digest plant biomass more efficiently than monogastric animals due to their symbiotic relationship with a complex microbiota residing in the rumen environment. What remains unclear is the relationship between the rumen microbial taxonomic and functional composition and feed efficiency (FE), especially in crossbred dairy cattle (Holstein x Gyr) raised under tropical conditions. In this study, we selected twenty-two F1 Holstein x Gyr heifers and grouped them according to their residual feed intake (RFI) ranking, high efficiency (HE) (n = 11) and low efficiency (LE) (n = 11), to investigate the effect of FE on the rumen microbial taxa and their functions. Rumen fluids were collected using a stomach tube apparatus and analyzed using amplicon sequencing targeting the 16S (bacteria and archaea) and 18S (protozoa) rRNA genes. Alpha-diversity and beta-diversity analysis revealed no significant difference in the rumen microbiota between the HE and LE animals. Multivariate analysis (sPLS-DA) showed a clear separation of two clusters in bacterial taxonomic profiles related to each FE group, but in archaeal and protozoal profiles, the clusters overlapped. The sPLS-DA also revealed a clear separation in functional profiles for bacteria, archaea, and protozoa between the HE and LE animals. Microbial taxa were differently related to HE (e.g., Howardella and Shuttleworthia) and LE animals (e.g., Eremoplastron and Methanobrevibacter), and predicted functions were significatively different for each FE group (e.g., K03395?signaling and cellular process was strongly related to HE animals, and K13643?genetic information processing was related to LE animals). This study demonstrates that differences in the rumen microbiome relative to FE ranking are not directly observed from diversity indices (Faith?s Phylogenetic Diversity, Pielou?s Evenness, Shannon?s diversity, weighted UniFrac distance, Jaccard index, and Bray?Curtis dissimilarity), but from targeted identification of specific taxa and microbial functions characterizing each FE group. These results shed light on the role of rumen microbial taxonomic and functional profiles in crossbred Holstein × Gyr dairy cattle raised in tropical conditions, creating the possibility of using the microbial signature of the HE group as a biological tool for the development of biomarkers that improve FE in ruminants. 650 $aBovino 650 $aGado Leiteiro 653 $aComposição microbiana 653 $aEficiência alimentar 653 $aMicrobiota 700 1 $aCAMPOS, M. M. 700 1 $aDIAS, R. J. P. 700 1 $aLIU, J. 700 1 $aGUO, W. 700 1 $aPEREIRA, L. G. R. 700 1 $aMACHADO, M. A. 700 1 $aREIS, D. R. de L. 700 1 $aGUAN, L. L. 700 1 $aGARNSWORTHY, P. C. 700 1 $aNEVES, A. L. A. 773 $tFrontiers in Microbiology$gv. 13, 1025173, 2022.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
01/12/2021 |
Data da última atualização: |
07/12/2021 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
ÁVILA, B. P.; PEREIRA, A. M.; GULARTE, M. A.; EICHOLZ, E. D. |
Afiliação: |
BIANCA PIO ÁVILA; ALINE MACHADO PEREIRA; MÁRCIA AROCHA GULARTE; EBERSON DIEDRICH EICHOLZ, CPACT. |
Título: |
Caracterização sensorial do milho BRS 015 Farináceo Branco e seu uso potencial em produtos de panificação. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Pelotas: Embrapa Clima Temperado, 2021. |
Páginas: |
17 p. |
Série: |
(Embrapa Clima Temperado. Comunicado Técnico, 385). |
ISSN: |
1516-8654 |
Idioma: |
Português |
Notas: |
ODS 2. |
Conteúdo: |
Segundo a Organização das Nações Unidas para Alimentação e Agricultura, o aumento da produção de alimentos, necessita do resgate da diversidade genética com mais pesquisas em nível local e nacional. Neste sentido, a qualidade alimentar, aliada a produção sustentável, torna-se uma das principais preocupações da pesquisa agrícola. |
Palavras-Chave: |
BRS 015. |
Thesagro: |
Análise de Alimento; Análise Organoléptica; Milho. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228341/1/COMUNICADO-TECNICO-385.pdf
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Marc: |
LEADER 01077nam a2200241 a 4500 001 2136926 005 2021-12-07 008 2021 bl uuuu u0uu1 u #d 022 $a1516-8654 100 1 $aÁVILA, B. P. 245 $aCaracterização sensorial do milho BRS 015 Farináceo Branco e seu uso potencial em produtos de panificação.$h[electronic resource] 260 $aPelotas: Embrapa Clima Temperado$c2021 300 $a17 p. 490 $a(Embrapa Clima Temperado. Comunicado Técnico, 385). 500 $aODS 2. 520 $aSegundo a Organização das Nações Unidas para Alimentação e Agricultura, o aumento da produção de alimentos, necessita do resgate da diversidade genética com mais pesquisas em nível local e nacional. Neste sentido, a qualidade alimentar, aliada a produção sustentável, torna-se uma das principais preocupações da pesquisa agrícola. 650 $aAnálise de Alimento 650 $aAnálise Organoléptica 650 $aMilho 653 $aBRS 015 700 1 $aPEREIRA, A. M. 700 1 $aGULARTE, M. A. 700 1 $aEICHOLZ, E. D.
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