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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
11/01/2008 |
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Data da última atualização: |
21/08/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
STADNIK, M. J.; BETTIOL, W. |
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Afiliação: |
Marciel João Stadnik, Departamento de Fitotecnia-Universidade Federal de Santa Catarina; WAGNER BETTIOL, CNPMA. |
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Título: |
Association between lipoxygenase and peroxidase activity and systemic protection of cucumber plants against Podosphaera xanthii induced by Oudemansiella canarii extracts. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Journal of Plant Diseases and Protection, Stuttgart, v.114, n.1, p.9-13, 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
This study was done to determine the relationship between the changes in lipoxygenase (LOX) and peroxidase (POX) activity and the protection of cucumber ( Cucumis sativus ) against powdery mildew ( Podosphaera xanthii ) induced by hexane or ethyl acetate extracts of basidiocarps of Oudemansiella canarii . At the two-leaf stage, only the primary leaves of the greenhouse grown cucumber plants were sprayed with either extract. The secondary leaves were inoculated at 1, 4, 7 or 10 days after the spray and the number of powdery mildew colonies were counted after 6 days. The LOX and POX activity in secondary leaves was determined 24 h after inoculation.In vitroantimicrobial effect of the extracts was tested against Bacillus and Cladosporium . Maximum disease reduction (85%) occurred when plants were inoculated one day after applying hexane extract. The disease reduction then decreased abruptly to 30% on the 4day, but then increased again when plants were inoculated seven or ten days after treatment. LOX activity increased only by the hexane extract application, but not by the pathogen. POX activity was higher in inoculated plants previously treated with the hexane extract. On the other hand, ethyl acetate extract did not affect the enzyme activity and its protection efficacy decreased continuously with increasing time interval between treatment and inoculation. Although both extracts showed antimicrobial activityin vitro, the results suggest that apolar compound(s) from the basidiocarps of O. canarii also induce systemic resistance to powdery mildew. MenosThis study was done to determine the relationship between the changes in lipoxygenase (LOX) and peroxidase (POX) activity and the protection of cucumber ( Cucumis sativus ) against powdery mildew ( Podosphaera xanthii ) induced by hexane or ethyl acetate extracts of basidiocarps of Oudemansiella canarii . At the two-leaf stage, only the primary leaves of the greenhouse grown cucumber plants were sprayed with either extract. The secondary leaves were inoculated at 1, 4, 7 or 10 days after the spray and the number of powdery mildew colonies were counted after 6 days. The LOX and POX activity in secondary leaves was determined 24 h after inoculation.In vitroantimicrobial effect of the extracts was tested against Bacillus and Cladosporium . Maximum disease reduction (85%) occurred when plants were inoculated one day after applying hexane extract. The disease reduction then decreased abruptly to 30% on the 4day, but then increased again when plants were inoculated seven or ten days after treatment. LOX activity increased only by the hexane extract application, but not by the pathogen. POX activity was higher in inoculated plants previously treated with the hexane extract. On the other hand, ethyl acetate extract did not affect the enzyme activity and its protection efficacy decreased continuously with increasing time interval between treatment and inoculation. Although both extracts showed antimicrobial activityin vitro, the results suggest that apolar compound(s) from the basidi... Mostrar Tudo |
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Palavras-Chave: |
Extrato fúngico; Indução de resistência. |
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Thesagro: |
Pepino. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02169naa a2200169 a 4500
001 1015915
005 2015-08-21
008 2007 bl uuuu u00u1 u #d
100 1 $aSTADNIK, M. J.
245 $aAssociation between lipoxygenase and peroxidase activity and systemic protection of cucumber plants against Podosphaera xanthii induced by Oudemansiella canarii extracts.
260 $c2007
520 $aThis study was done to determine the relationship between the changes in lipoxygenase (LOX) and peroxidase (POX) activity and the protection of cucumber ( Cucumis sativus ) against powdery mildew ( Podosphaera xanthii ) induced by hexane or ethyl acetate extracts of basidiocarps of Oudemansiella canarii . At the two-leaf stage, only the primary leaves of the greenhouse grown cucumber plants were sprayed with either extract. The secondary leaves were inoculated at 1, 4, 7 or 10 days after the spray and the number of powdery mildew colonies were counted after 6 days. The LOX and POX activity in secondary leaves was determined 24 h after inoculation.In vitroantimicrobial effect of the extracts was tested against Bacillus and Cladosporium . Maximum disease reduction (85%) occurred when plants were inoculated one day after applying hexane extract. The disease reduction then decreased abruptly to 30% on the 4day, but then increased again when plants were inoculated seven or ten days after treatment. LOX activity increased only by the hexane extract application, but not by the pathogen. POX activity was higher in inoculated plants previously treated with the hexane extract. On the other hand, ethyl acetate extract did not affect the enzyme activity and its protection efficacy decreased continuously with increasing time interval between treatment and inoculation. Although both extracts showed antimicrobial activityin vitro, the results suggest that apolar compound(s) from the basidiocarps of O. canarii also induce systemic resistance to powdery mildew.
650 $aPepino
653 $aExtrato fúngico
653 $aIndução de resistência
700 1 $aBETTIOL, W.
773 $tJournal of Plant Diseases and Protection, Stuttgart$gv.114, n.1, p.9-13, 2007.
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