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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/11/2004 |
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Data da última atualização: |
23/11/2015 |
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Autoria: |
CARVALHO, C. H. S.; ZEHR, U. B.; GUNARATNA, N.; ANDERSON, J.; KONONOWICZ, H. H.; HODGES, T. K.; AXTELL, J. D. |
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Afiliação: |
CARLOS HENRIQUE S DE CARVALHO, Embrapa Milho e Sorgo; Usha B. Zehr, Purdue University; Nilupa Gunaratna, Purdue University; Joseph Anderson, Purdue University; Halina H. Kononowicz, Purdue University; Thomas K. Hodges, Purdue University; John D. Axtell, Purdue University. |
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Título: |
Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 27, n. 2, p. 259-269, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection. MenosThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculu... Mostrar Tudo |
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Thesagro: |
Sorgo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32593/1/Agrobacterium-mediated.pdf
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Marc: |
LEADER 02225naa a2200205 a 4500
001 1488374
005 2015-11-23
008 2004 bl uuuu u00u1 u #d
100 1 $aCARVALHO, C. H. S.
245 $aAgrobacterium-mediated transformation of sorghum$bfactors that affect transformation efficiency.$h[electronic resource]
260 $c2004
520 $aThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection.
650 $aSorgo
700 1 $aZEHR, U. B.
700 1 $aGUNARATNA, N.
700 1 $aANDERSON, J.
700 1 $aKONONOWICZ, H. H.
700 1 $aHODGES, T. K.
700 1 $aAXTELL, J. D.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 27, n. 2, p. 259-269, 2004.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Origem |
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Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
19/01/2019 |
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Data da última atualização: |
02/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
DIAS, R. de C. S.; SANTOS, J. S. |
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Afiliação: |
RITA DE CASSIA SOUZA DIAS, CPATSA; JOICE SIMONE DOS SANTOS. |
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Título: |
Panorama nacional da produção de melancia. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Revista Campo & Negócios HF, v. 14, n. 163, p. 44-48, jan. 2019. |
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ISSN: |
2359-5310 |
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Idioma: |
Português |
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Conteúdo: |
Informe técnico: Panorama nacional da produção de melancia; características atrativas ao mercado consumidor; Tecnologias; Cultivares mais plantadas; Manejo da cultura; Adubação; Calagem; Irrigação; Espaçamento; Cuidados fitossanitários; Abelhas, agentes polinizadores; Colheita; Erros que devem ser evitados; Novidades; Melancia sem sementes; Enxertia em melancia; Ninchos de mercado; Custo; Rentabilidade. |
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Palavras-Chave: |
Cultivar plantada; Enxertia; Manejo da cultura; Mercado consumidor; Vale do São Francisco. |
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Thesagro: |
Comportamento de Variedade; Custo de Produção; Irrigação; Manejo; Melancia; Produção Agrícola; Sistema de Cultivo; Variedade. |
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Thesaurus NAL: |
Cost benefit analysis; Crop production; Watermelons. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196237/1/Rita-2019.pdf
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Marc: |
LEADER 01327naa a2200337 a 4500
001 2104558
005 2019-12-02
008 2019 bl uuuu u00u1 u #d
022 $a2359-5310
100 1 $aDIAS, R. de C. S.
245 $aPanorama nacional da produção de melancia.
260 $c2019
520 $aInforme técnico: Panorama nacional da produção de melancia; características atrativas ao mercado consumidor; Tecnologias; Cultivares mais plantadas; Manejo da cultura; Adubação; Calagem; Irrigação; Espaçamento; Cuidados fitossanitários; Abelhas, agentes polinizadores; Colheita; Erros que devem ser evitados; Novidades; Melancia sem sementes; Enxertia em melancia; Ninchos de mercado; Custo; Rentabilidade.
650 $aCost benefit analysis
650 $aCrop production
650 $aWatermelons
650 $aComportamento de Variedade
650 $aCusto de Produção
650 $aIrrigação
650 $aManejo
650 $aMelancia
650 $aProdução Agrícola
650 $aSistema de Cultivo
650 $aVariedade
653 $aCultivar plantada
653 $aEnxertia
653 $aManejo da cultura
653 $aMercado consumidor
653 $aVale do São Francisco
700 1 $aSANTOS, J. S.
773 $tRevista Campo & Negócios HF$gv. 14, n. 163, p. 44-48, jan. 2019.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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