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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
17/12/2013 |
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Data da última atualização: |
17/12/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COSTA, S. R. da; SANTOS, C. A. F. |
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Afiliação: |
S. R. da COSTA, Universidade Estadual de Feira de Santana, Feira de Santana, BA; CARLOS ANTONIO FERNANDES SANTOS, CPATSA. |
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Título: |
Allelic database and divergence among Psidium accessions by using microsatellite markers. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 12, n. 4, p. 6802-6812, 2013. |
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ISSN: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variability is not uniformly distributed among the 9 Brazilian states from where the accession were obtained. Obtaining a greater number of accessions by Brazilian states is recommended in order to have greater diversity among the species. MenosThis study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variabili... Mostrar Tudo |
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Palavras-Chave: |
Banco ativo de germoplasma; Dendrograma; Guava; Marcadores microssatélites; Melhoramento genético; Variabilidade genética. |
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Thesagro: |
Goiaba. |
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Thesaurus Nal: |
Breeding; Psidium. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/974120/1/Carlos2.pdf
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Marc: |
LEADER 02480naa a2200253 a 4500
001 1974120
005 2013-12-17
008 2013 bl uuuu u00u1 u #d
022 $a1676-5680
100 1 $aCOSTA, S. R. da
245 $aAllelic database and divergence among Psidium accessions by using microsatellite markers.$h[electronic resource]
260 $c2013
520 $aThis study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variability is not uniformly distributed among the 9 Brazilian states from where the accession were obtained. Obtaining a greater number of accessions by Brazilian states is recommended in order to have greater diversity among the species.
650 $aBreeding
650 $aPsidium
650 $aGoiaba
653 $aBanco ativo de germoplasma
653 $aDendrograma
653 $aGuava
653 $aMarcadores microssatélites
653 $aMelhoramento genético
653 $aVariabilidade genética
700 1 $aSANTOS, C. A. F.
773 $tGenetics and Molecular Research$gv. 12, n. 4, p. 6802-6812, 2013.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| 4. |  | TAVARES, S. C. C. de H. Biological control of mango crop diseases in Brazil. In: INTERNATIONAL MANGO SYMPOSIUM, 7., 2002, Recife. Program and abstracts... Fortaleza: Embrapa Agroindústria Tropical; Planaltina: Embrapa Cerrados; Cruz das Almas: Embrapa Mandioca e Fruticultura; Teresina: Embrapa Meio-Norte; Petrolina: Embrapa Semi-Árido; Recife: IPA, 2002. p. 58. (Embrapa Agroindústria Tropical. Documentos, 46).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 17. | | TAVARES, S. C. C. de H. (Ed.). Melão: fitossanidade. Brasília, DF: Embrapa Informação Tecnológica, 2002. 87 p. (Embrapa Informação Tecnológica. Frutas do Brasil, 25).| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Semiárido; Embrapa Unidades Centrais. |
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| 18. | | TAVARES, S. C. C. de H. Melão sem fungos. Cultivar HF, Pelotas, v. 2, n. 12, p. 29-34, fev./mar. 2002.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 229 | |
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