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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
29/11/2021 |
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Data da última atualização: |
29/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUES, C. I.; COSTA, D. M. da; SANTOS, A. C. V.; BATATINHA, M. J. M.; SOUZA, F. V. D.; SOUZA, E. H. de; BOTURA, M. B.; ALVES, C. Q.; SOARES, T. L.; BRANDÃO, H. N. |
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Afiliação: |
CAROLINE ISSLER RODRIGUES, UEFS; DIEGO MOTA DA COSTA, UEFS; ACIDÁLIA CARINE VIEIRA SANTOS, UFBA; MARIA JOSÉ MOREIRA BATATINHA, UFBA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EVERTON HILO DE SOUZA, UFRB; MARIANA BORGES BOTURA, UEFS; CLAYTON QUEIROZ ALVES, UEFS; TALIANE LEILA SOARES, UEFS; HUGO NEVES BRANDÃO, UEFS. |
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Título: |
Assessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Parasitology, v.285, 2020. |
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ISSN: |
1873-2550 |
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DOI: |
10.1016/j.vetpar.2020.109219 |
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Idioma: |
Inglês |
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Conteúdo: |
Species of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: B-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL-1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive. MenosSpecies of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to i... Mostrar Tudo |
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Thesagro: |
Abacaxi. |
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Categoria do assunto: |
-- |
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Marc: |
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100 1 $aRODRIGUES, C. I.
245 $aAssessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts.$h[electronic resource]
260 $c2020
520 $aSpecies of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: B-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL-1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive.
650 $aAbacaxi
700 1 $aCOSTA, D. M. da
700 1 $aSANTOS, A. C. V.
700 1 $aBATATINHA, M. J. M.
700 1 $aSOUZA, F. V. D.
700 1 $aSOUZA, E. H. de
700 1 $aBOTURA, M. B.
700 1 $aALVES, C. Q.
700 1 $aSOARES, T. L.
700 1 $aBRANDÃO, H. N.
773 $tVeterinary Parasitology$gv.285, 2020.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 43 | |
| 4. |  | FREITAS, J. G.; SILVA, J. C. A. da; MOTA, J. R.; SILVA, G. A. da. Comportamento da cultivar de mamona brs energia cultivada sob duas densidades de plantio em Irecê, BA. In: CONGRESSO BRASILEIRO DE MAMONA, 4.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 1., 2010, João Pessoa. Inclusão social e energia: anais. Campina Grande: Embrapa Algodão, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 10. | | LISBOA, P. L. B.; GOMES, J. I.; SILVA, J. C. A. da; SANTOS, G. M. dos A. dos. Identificação macroscópica de madeiras amazônicas. In: CONGRESSO NACIONAL DE BOTÂNICA, 39., 1988, Belém, PA. Resumos... Belém, PA: SBB: MPEG, 1988. v. 2, p. 42-43.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | DINIZ NETO, M. A.; SILVA, I. DE F. DA; CAVALCANTE, A. C. P.; SILVA, A. G. DA; SILVA, J. C. A. da; DINIZ, B. L. M. T. Avaliação de mudas de oiticica submetidas a diferentes doses de composto de mamona e níveis de salinidade. In: CONGRESSO BRASILEIRO DE MAMONA, 6.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 3., 2014, Fortaleza. Energia e segurança alimentar na agricultura familiar: anais. Campina Grande, PB: Embrapa Algodão, 2014. p. 46| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 12. | | DINIZ NETO, M. A.; SILVA, I. DE F. DA; CAVALCANTE, A. C. P.; SILVA, A. G. DA; SILVA, J. C. A. da; DINIZ, B. L. M. T. Amendoim br1 cultivado com diferentes compostos orgânicos e aplicação de biofertilizante. In: CONGRESSO BRASILEIRO DE MAMONA, 6.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 3., 2014, Fortaleza. Energia e segurança alimentar na agricultura familiar: anais. Campina Grande, PB: Embrapa Algodão, 2014. p. 36| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 14. | | CARTAXO, W. V.; ALVES, I.; VALE, D. G.; OLIVEIRA, G. dos S.; SILVA, J. C. A. da; CARDOSO, G. D.; GUIMARAES, F. M. Caso de sucesso do algodão colorido agroecológico na agricultura familiar da Paraíba. In: CONGRESSO BRASILEIRO DO ALGODÃO, 10., 2015, Foz do Iguaçu. Resumos. Brasília, DF: ABRAPA, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 15. | | CAVALCANTE, A. C. P.; DINIZ NETO, M. A.; SILVA, A. G. DA; DINIZ, B. L. M. T.; SILVA, J. C. A. da; SILVA, A. R. DA. Compostos orgânicos e aplicação de biofertilizante enriquecido no algodoeiro herbácio cv. brs 8h. In: CONGRESSO BRASILEIRO DE MAMONA, 6.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 3., 2014, Fortaleza. Energia e segurança alimentar na agricultura familiar: anais. Campina Grande, PB: Embrapa Algodão, 2014. p. 53| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 16. | | VALE, D. G.; SILVA, O. R. R. F.; CARTAXO, W. V.; SOFIATTI, V.; GUIMARAES, F. M.; OLIVEIRA, G. dos S.; CARDOSO, G. D.; SILVA, J. C. A. da. Deslintamento de sementes para o cultivo do algodão orgânico ou agroecológico. In: CONGRESSO BRASILEIRO DO ALGODÃO, 11., 2017, Maceió. Resumos... Inovação e rentabilidade na cotonicultura: resumos... Brasília, DF: Associação Brasileira dos Produtores de Algodão - Abrapa, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 43 | |
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